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OfflineMr. Mushie

Registered: 01/16/20
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A2G jars are all contaminating
    #27039311 - 11/14/20 06:46 PM (3 years, 3 months ago)

I inoculated 50+ jars from T3 plates over the course of the last two weeks.

All jars show good initial growth and then stall out completely. Upon shaking, it's evident that the growth has solidified in one area and resembles a dry chewing gum texture in all of my jars.




They all look similar to these jars above before stalling and creating this large gum like clump.





The jars all smell sour when opened for disposal. Here are some pics of plates i've used to inoculate jars the other night, I will say that plates I had used on the earlier jars definitely looked "better" than these.








I imagine that my agar plates are contaminated and that i'm not experienced enough to notice the contams. A couple of my plates had rhizomorphic growth after T1 and T2 but most don't have any defined sectors in the T3 plates.





I don't believe my grain prep is the issue as I made some control jars 5 weeks ago and after a month of sitting uninoculated they smelled like fresh oats.

Any tips would be greatly appreciated, clean spawn is the hardest part of cultivation for me!

Thank you for your time.


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OnlineCocaineBuffet
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Re: A2G jars are all contaminating [Re: Mr. Mushie] * 1
    #27039327 - 11/14/20 06:53 PM (3 years, 3 months ago)

Sour smell is not a good sign but clumps of mycelium isn't a bad one, at least in my grain. I have to break it up by hand after scooping out the chunks to get even distribution with the substrate.

Shaking is to encourage displacement of grains so they can spread out and colonize in more spaces.

If it was me personally I would not have an issue putting a bit of that rhizo growth to grain (your plate towards the bottom of your post) maybe put it to a new plate to be extra sure. I am still trying to lower my contam rates but that is my opinion.


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OfflineMr. Mushie

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Re: A2G jars are all contaminating [Re: CocaineBuffet]
    #27039494 - 11/14/20 08:03 PM (3 years, 3 months ago)

These clumps stall entirely and only colonize a small area around where the agar wedge was dropped into the jar. It's not the positive clumping that you speak of.


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InvisibleJosex
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Re: A2G jars are all contaminating [Re: Mr. Mushie] * 1
    #27039500 - 11/14/20 08:09 PM (3 years, 3 months ago)

You have to switch grain. If your problem persists then start to suspect your agar, but I believe your problem stems from those oats. Maybe you got a bad batch, maybe you didn't prep them right, maybe it was an insufient PC cycle, maybe improper venting or a combination of these.

Those cultures look sketch, though.


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OfflineMr. Mushie

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Re: A2G jars are all contaminating [Re: Josex]
    #27039634 - 11/14/20 10:15 PM (3 years, 2 months ago)

I always vent for 10 minutes minimum. PC'd @16 psi for 2 hours.

I'm going to try WBS next and eventually Rye/wheat when I can find a solid supplier one state over.

I have a feeling it's my agar.

What's your grain of choice Josex?


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Re: A2G jars are all contaminating [Re: Mr. Mushie]
    #27039667 - 11/14/20 11:04 PM (3 years, 2 months ago)

Wheat and millet. I can't get wbs but lots of people like them too. I like any grain but oats, really.

Even if your agar was bacterial, it ain't normal for myc to stall like that. Are those oats organic by any chance?


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OfflineMr. Mushie

Registered: 01/16/20
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Re: A2G jars are all contaminating [Re: Josex]
    #27039702 - 11/14/20 11:38 PM (3 years, 2 months ago)

They're not organic. They're actually the same oats i've seen some of the TC's use on here... I suppose I could've got a bad batch.



Out of 50+ jars, only one has fully colonized and it smelled off (not like fresh mushrooms at all) but I still spawned it to a shoebox for science.



Edited by Mr. Mushie (11/15/20 03:04 AM)


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Offlinedrinkkykeon
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Re: A2G jars are all contaminating [Re: Josex]
    #27039704 - 11/14/20 11:39 PM (3 years, 2 months ago)

How are your jars ventilated? I was using a tiny hole on the top of my jars covered with micropore tape and had similar issues. I reamed out the hole and the stalls went away.


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OfflineMr. Mushie

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Re: A2G jars are all contaminating [Re: drinkkykeon]
    #27039711 - 11/14/20 11:45 PM (3 years, 2 months ago)

1/4 hole stuffed tight with poly-fil.



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Offlinedrinkkykeon
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Re: A2G jars are all contaminating [Re: Mr. Mushie]
    #27039720 - 11/14/20 11:49 PM (3 years, 2 months ago)

Looks good. I don't think your agar is contaminated. Do you use a SAB or flowhood?


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 3 months, 11 days
Re: A2G jars are all contaminating [Re: drinkkykeon]
    #27039731 - 11/14/20 11:59 PM (3 years, 2 months ago)

I use a 116qt SAB for now. Going to order a Myers FFU when they're back in stock. I hate being hunched over for 4 hours doing agar transfers in my SAB :facepalm:


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