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InvisibleBallHairDandruf
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URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!!
    #27039393 - 11/14/20 07:22 PM (3 years, 2 months ago)

Ok so I have had dozens of good yields and harvests in the past. For some reason my last 6 tubs i have done have contaminated over a period of a 1 1/2 months. All have had the same exact contam. As soon as i notice it i dump it in my backyard and let it decompose or whatever, as long as it isnt near my other grows. So im just wondering why the fuck all of them have contamed. Here is a list of some stuff i been doing

I have been waiting for a while after my jars fully colonize, sometimes a few weeks

I have been opening my tubs every few days so my subs can air out a little (because some have taken a really long time do to me being retarded and doing very low ratios)

I just went on a trip for 2 days and had the window open the whole time which is in my room.

If i ever detect any signs of contamination in my jars they are thrown out and the reason i wait a few weeks after full colonization is because i am trying to see if any will show. 

They all show up out of nowhere in huge clumps in my tubs, like 1/9-1/5 of the tubs.


That is not my picture but i found it in the contam forum and it looks close to it.

I have had black mold running around my house the last 2 months but it has never contaminated a colonizing sub

PLEASE HELP ME FIND OUT WHY THE FUCK MY SHIT KEEPS CONTAMINATING, THIS SHIT IS PISSING ME OFF. 

My tubs are air tight btw


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InvisibleModularMind
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf]
    #27039432 - 11/14/20 07:38 PM (3 years, 2 months ago)

How do you inoculate grain spawn?


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InvisibleThe Fresh PrintsS
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: ModularMind]
    #27039447 - 11/14/20 07:41 PM (3 years, 2 months ago)

Quote:

BallHairDandruf said:

My tubs are air tight btw




Air tight is not what you want for a fruiting chamber but that isn't the reason you're getting contams on the reg. Are these tubs all the same culture?


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: ModularMind]
    #27039449 - 11/14/20 07:42 PM (3 years, 2 months ago)

Syringes


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: The Fresh Prints]
    #27039454 - 11/14/20 07:44 PM (3 years, 2 months ago)

yup. All the same culture. rn im using albino a+. I got the ones that the majority of people use. Blue foam acts as a gasket kind of. Maybe not air tight but definetly does not let air in easy if it does.


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InvisibleModularMind
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf]
    #27039458 - 11/14/20 07:45 PM (3 years, 2 months ago)

Quote:

BallHairDandruf said:
Syringes



Quote:

BallHairDandruf said:
yup. All the same culture. rn im using albino a+.




A liquid culture?


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: The Fresh Prints]
    #27039461 - 11/14/20 07:46 PM (3 years, 2 months ago)



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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: ModularMind]
    #27039463 - 11/14/20 07:46 PM (3 years, 2 months ago)

Quote:

ModularMind said:
Quote:

BallHairDandruf said:
Syringes



Quote:

BallHairDandruf said:
yup. All the same culture. rn im using albino a+.




A liquid culture?



yessir. LC


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InvisibleThe Fresh PrintsS
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf] * 1
    #27039488 - 11/14/20 07:57 PM (3 years, 2 months ago)

Bad LC or bad grain prep probably. Send pics of your spawn if you can.


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: The Fresh Prints]
    #27039489 - 11/14/20 08:01 PM (3 years, 2 months ago)

Quote:

Genghis Chron said:
Bad LC or bad grain prep probably. Send pics of your spawn if you can.



My spawn or my sub? I dont have any spawn that mycelium on it that you can see, as i just did a new batch. Are you not supposed to open your sub at all until its ready to fruit


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OfflineLogicaL ChaosM
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf]
    #27039576 - 11/14/20 09:13 PM (3 years, 2 months ago)

Did u clean up your spores on agar before adding to your LC?

The pic in your OP (the reference pic) looks like Trichoderma, a very common mold containiment.


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InvisibleZiran
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf] * 1
    #27039581 - 11/14/20 09:16 PM (3 years, 2 months ago)

you are supposed to inoculate in this order normally.

Spore Syringe/Spore Print/Spore Swab ----> Agar ----> Clean Culture on Agar -----> Grains -----> Spawned to substrate

You can also do this

Spore Syringe/Spore Print/Spore Swab ----> Agar ----> Clean Culture on Agar -----> LC -----> Massive amount of grains ----> Substrate

or even

Spore Syringe/Spore Print/Spore Swab ----> Agar ----> Clean Culture on Agar -----> Grains -----> Grain to Grain -----> Spawned to substrate


alternatively, Old school methods would go like this

Spore Syringe -----> Grains ----> Substrate


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: LogicaL Chaos]
    #27039723 - 11/14/20 11:50 PM (3 years, 2 months ago)

Quote:

LogicaL Chaos said:
Did u clean up your spores on agar before adding to your LC?

The pic in your OP (the reference pic) looks like Trichoderma, a very common mold containiment.



yes my spores were clean. Ive heard people say there is no way it will contam unless it was already in the grain, but im really not sure. I was thinking it was Trich. Is letting air in to your sub ok???


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InvisibleThe Fresh PrintsS
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf] * 1
    #27039736 - 11/15/20 12:06 AM (3 years, 2 months ago)

Yes. No need to suffocate your tubs. Drill some holes or crack the lid.


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InvisibleBallHairDandruf
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: The Fresh Prints]
    #27039747 - 11/15/20 12:20 AM (3 years, 2 months ago)

it seems whenever i do open it it contams...


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InvisibleThe Fresh PrintsS
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Re: URGENT!! PLEASE HELP I HAVE A HUGE PROBLEM!! [Re: BallHairDandruf] * 1
    #27039755 - 11/15/20 12:27 AM (3 years, 2 months ago)

That's just a coincidence. If you're getting contams before the first flush, your problem is probably dirty spawn.


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