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OfflineChorizogrande
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Agar-- when do you like to transfer?
    #27037532 - 11/13/20 06:24 PM (3 years, 2 months ago)

Having fun with Agar.  I haven't read too many different things on it. Just a few teks. Curious when everyone likes to transfer? Do you go by day count  or when the Agar colo


I'm currently on my 3rd transfer from spore solution. Did 2 plates each time. This time will take one plate and transfer to 6. Then introduce those to grain.


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OfflineBrian Jones
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Re: Agar-- when do you like to transfer? [Re: Chorizogrande]
    #27037600 - 11/13/20 06:57 PM (3 years, 2 months ago)

For sure before the colonization reaches the edge. I usually like to inoculate jars with wedges and take some transfers at the same time. If I'm only taking transfers, I like to do it when the colonization is about the size of a quarter.

I have had much higher success rates since I started making more plates. Agar is not fool proof and sometimes a contam is lurking that you can't see. Plus there's a chance for technique mistakes in the SAB. I usually use two plates for seven grain jars. If you have nice looking plates that you don't need till next week throw them in the refrigerator to stall the growth. I also like the technique from Bodhi's video keeping several exacto knives in a shot glass with a little alcohol next to the SAB and alternating them before flaming.


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OfflineChorizogrande
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Re: Agar-- when do you like to transfer? [Re: Brian Jones] * 1
    #27040198 - 11/15/20 09:14 AM (3 years, 2 months ago)

Right on man. Thanks for the info.


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