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OfflineGioLeos
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    #27036237 - 11/13/20 01:29 AM (4 years, 2 months ago)

old

Edited by GioLeos (12/02/20 03:50 PM)

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OfflineGioLeos
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old [Re: GioLeos]
    #27036251 - 11/13/20 01:44 AM (4 years, 2 months ago)

old

Edited by GioLeos (12/02/20 03:50 PM)

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OfflineMH5109
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Re: Quick question on ms [Re: GioLeos]
    #27036280 - 11/13/20 02:38 AM (4 years, 2 months ago)

I have never used bags before but I would never inject spores into a bag of grain. My suggestion is make some brf cakes and inject around 1-2cc into that, and get some petris and take a clone to agar. A couple ccs into the bag would probably be enough but why risk it when you can make 5 or so brf cakes to start and get a viable clone.


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OfflineGioLeos
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old [Re: GioLeos]
    #27036371 - 11/13/20 04:16 AM (4 years, 2 months ago)

old

Edited by GioLeos (12/02/20 03:50 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Quick question on ms [Re: GioLeos]
    #27036391 - 11/13/20 04:57 AM (4 years, 2 months ago)

Spores to grain? 0mLs

Use 1-2 if you must

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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Re: Quick question on ms [Re: GioLeos]
    #27036783 - 11/13/20 10:14 AM (4 years, 2 months ago)

You can use BRF cakes as spawn no problem.


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Offlinestarbones
I'm an alien, I eat uranium.
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Registered: 03/04/20
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Re: Quick question on ms [Re: coversall]
    #27036950 - 11/13/20 12:06 PM (4 years, 2 months ago)

That's a very quick way to find out why you shouldn't put MS to grain.

The ONLY time I put MS to grain ever is in 500ml grain jars filled 2/3 of the way because I know I'm going to lose more than a few and the ONLY reason I ever do this is to get fruits up in a shoebox as fast as possible to find something to clone and I can't be fucked to do PF Tek.

MS syringes are dirty because the fruit was not grown in sterile conditions and the prints they're made from are covered in everything from yeasts to molds to bacteria.

Proper procedure would be to germinate it on agar, make transfers away from the original plate to clean it up and then I guess you could make an LC if you're feeling fruity. If you're serious about getting into this hobby learn agar now before you start to pine for things you can only get from a swab.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.



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Invisiblemushhead
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Re: Quick question on ms [Re: bodhisatta]
    #27037078 - 11/13/20 01:26 PM (4 years, 2 months ago)

Quote:

bodhisatta said:
Spores to grain? 0mLs

Use 1-2 if you must



:rofl:
bod u amaze.

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