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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Storing leftover petri dishes in sleeve?
#27036537 - 11/13/20 07:54 AM (3 years, 2 months ago) |
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Guys,
I have a sterilized sleeve of 20 disposable petri dishes. I want to pour 10 agar plates for a small project. I only grow a few times a year. If I pour all 20 plates, the leftover 10 plates are going to dry out before I'll use them. That seems like a waste.
After opening the sleeve in my SAB and removing the 10 plates for pouring, I plan to close and roll up the opened end of the sleeve and tape it shut.
Is this procedure sufficient to ensure that the leftover plates remain sterile in the sleeve? Is there a better method for storing leftover plates in a sleeve?
Please impart your collective wisdom.
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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
Last seen: 6 months, 15 days
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Re: Storing leftover petri dishes in sleeve? [Re: tomcards]
#27036541 - 11/13/20 07:56 AM (3 years, 2 months ago) |
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I've stored non-parafilmed dishes in their original opened sleeves and it went fine, but I've only done it for like 24 hours. However, undisturbed and in that sleeve I see no reason why they should not stay OK. Just absolutely make sure to not separate lid and bottom even for a moment when removing your bottom ten of course.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Storing leftover petri dishes in sleeve? [Re: tomcards]
#27036547 - 11/13/20 07:59 AM (3 years, 2 months ago) |
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Storing blank petri dishes for later use is common practice. Click the link in my signature and see how I wrap a sleeve of plates for later use.
I personally don't like putting them back in the sleeve because I find the process to be clumsy. Some people put the blanks unwrapped into freezer ziplocks, I prefer my method. It's super easy, sturdy, practical.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Storing leftover petri dishes in sleeve? [Re: Stipe-n Cap]
#27036558 - 11/13/20 08:09 AM (3 years, 2 months ago) |
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Thanks for your prompt responses guys! Very helpful and reassuring.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Storing leftover petri dishes in sleeve? [Re: tomcards]
#27086855 - 12/13/20 07:45 AM (3 years, 1 month ago) |
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An update.
I poured plates using the previously-opened-and-resealed sleeve of plates.
Two hours after pouring, the lids of the two plates that were nearest to the resealed opening of the sleeve had hearty contamination growing on them. After a mere two hours! I've never seen anything like it.
Clearly, my handling and resealing technique were inadequate.
I promptly pitched them.
The remaining plates were, and still are, clean.
Consider this a cautionary tale. Be very careful when handling and resealing a sleeve of plates. Despite your best laid plans and execution, you may lose a plate or two. While not a significant loss, it will motivate you to improve your technique to prevent all loss in the future.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Storing leftover petri dishes in sleeve? [Re: tomcards] 1
#27086914 - 12/13/20 08:37 AM (3 years, 1 month ago) |
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I have never lost a plate:
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