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Onlineblack strat
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Re: Check out my agar, please? [Re: karri0n]
    #27045809 - 11/18/20 05:59 PM (8 days, 7 hours ago)

very very well said at the end, my brother. I notice my little bumps of disappointment and frustration are short-lived, and then I'm wanting to start messing around with this shit more and more. for example, my prints came in today. stoked. b+, gt, ecuador, & pes amazonian(?).  already got the sterile swabs and fresh plates ready to go. gonna go smallll this time hah. I'm listening!

hey, is there any good argument for using a loop over a sterile swab? entirely curious. I'm gonna swab em tonight tho.


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Offlinekarri0n
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Re: Check out my agar, please? [Re: black strat]
    #27046242 - 11/18/20 09:28 PM (8 days, 3 hours ago)

I tried loop the other day after getting mold on one of the plates I did with the swab.

My thought was possibly that I introduced contam from the foil wrap which I didn't remove fully but only peeled down partially, and that a mood spore had landed on it at one of the times I opened the jar to take a swab out.

A solid metal wire that I flame the entire thing can't have that issue.

It will depend on the results, but I had an easier time controlling the sterile swabs than the loop. I see people using both.


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Offlinejake103
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Re: Check out my agar, please? [Re: karri0n]
    #27046360 - 11/18/20 10:28 PM (8 days, 2 hours ago)

Can anyone tell me once the agar plates are fully colonized, do they go in the fridge or stay out in room temperature? I have not been able to find this simple answer anywhere, thank you! Also, this was my agar at 11 days, don't worry about the black, that is electrical tape as I find the dishes flimsy and more subject to contamns if I don't secure them.


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Offlinejake103
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Re: Check out my agar, please? [Re: jake103]
    #27046364 - 11/18/20 10:29 PM (8 days, 2 hours ago)



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OfflineGrenik
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Re: Check out my agar, please? [Re: jake103]
    #27046379 - 11/18/20 10:42 PM (8 days, 2 hours ago)

I put plates I want to store in the refrigerator.  I have only removed one from cold storage and used it so I am not an expert.  Transfer before they get completely filled and then store cold.

Does your plate have condensation on the top? If so, put a warm glass or bowl of water on it to slightly heat the lid and get rid of the condensation.  Then take another picture and post it. Was it from a spore print?


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OfflineGrinchGrower
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Re: Check out my agar, please? [Re: jake103]
    #27046482 - 11/18/20 11:29 PM (8 days, 1 hour ago)

Quote:

jake103 said:





That colonization does not looks very good imo...
You should transfer it at least once to get some better growth.


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Offlinejake103
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Re: Check out my agar, please? [Re: GrinchGrower]
    #27047355 - 11/19/20 03:31 PM (7 days, 9 hours ago)

Ummm...this is only at 11 days. And no, I did not have condensation on these particular dishes. I tried the hot water method suggested for some of the newer ones but it did not work. I have them incubating with my jars. The ones pictured are from a spore syringe.


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Offlinejake103
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Re: Check out my agar, please? [Re: jake103]
    #27047358 - 11/19/20 03:31 PM (7 days, 9 hours ago)



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OfflinePhony Phone
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Re: Check out my agar, please? [Re: jake103]
    #27047399 - 11/19/20 04:13 PM (7 days, 8 hours ago)

Quote:

jake103 said:
Can anyone tell me once the agar plates are fully colonized, do they go in the fridge or stay out in room temperature? I have not been able to find this simple answer anywhere, thank you! Also, this was my agar at 11 days, don't worry about the black, that is electrical tape as I find the dishes flimsy and more subject to contamns if I don't secure them.




leave in room temp in a baggie during colonization
at 2/3 of colonization put in the refrigerator. dont let it get at 100% plate colonization or it gets messy


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Offlinejake103
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Re: Check out my agar, please? [Re: Phony Phone]
    #27047932 - 11/19/20 09:11 PM (7 days, 3 hours ago)

OK, thank you! I have been too nervous to transfer them because I don't want to contamn and this is so far the best looking agar I have been able to colonize. All white, no other colors. I will put them in baggies as suggested and transfer soon. Also, what do you mean by they get messy?


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OfflinePhony Phone
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Re: Check out my agar, please? [Re: jake103]
    #27048021 - 11/19/20 10:20 PM (7 days, 2 hours ago)

at full colonization the mycelium tends to reach for the plate walls and crawl out


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Onlineblack strat
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Re: Check out my agar, please? [Re: Phony Phone]
    #27048205 - 11/20/20 01:43 AM (6 days, 23 hours ago)

48 hrs since first transfer....



can't even tell you how appreciative I am of you guys offering opinions and kind words..

I jus reeeally am curious to hear what maybe a trusted cultivator, or someone who's been doin this shit for a good while thinks about these plates..

I'm a slightly skeptical person as it is, but I jus can't say out of all the pics I've seen on this site of mycelium, that my plates look similar to theirs. I'm not seeing that 'ropey', uniformed length, and fan-like shape that basically all yalls tend to have. lol please tell me I'm wrong.


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InvisibleCosmicKramer
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Re: Check out my agar, please? [Re: black strat]
    #27048693 - 11/20/20 12:14 PM (6 days, 12 hours ago)

Dude, that is not myc.  Pitch them and start over.


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Onlineblack strat
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Re: Check out my agar, please? [Re: CosmicKramer]
    #27048833 - 11/20/20 01:41 PM (6 days, 11 hours ago)

lol yeah, sounds about right.


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Offlinekarri0n
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Re: Check out my agar, please? [Re: black strat]
    #27048835 - 11/20/20 01:43 PM (6 days, 11 hours ago)

Quote:

black strat said: I'm not seeing that 'ropey', uniformed length, and fan-like shape that basically all yalls tend to have. lol please tell me I'm wrong.





You're talking about rhizomorphic growth, I call the shape lightning roots. From spores there can be a hundred of those rhizomes crawling all over one another so it just looks like a mess. Once you isolate strains out by taking very small tissue samples on transfers, they tend to become more rhizomorphic.

Myc can take lots of forms. The common three are rhizomorphic, cottony, and leathery. Cubensis can make all three.

Not even all cube strains turn rhizomorphic. You can have a tomentose strain that produces killer fruits and a rhizo strain that never fruits at all, from the same spores from one fruit. USUALLY, rhizomorphic growth is preferred I believe due to its colonization speed and possibly ease of identification.

I really can't say either way with those. It hasn't turned green or gray or pink so that's definitely a plus. It looks wiry, bit also fluffy. I kinda thought mold from the start but I can very easily be wrong and don't want you to throw it out on my bad advice.

You could smell one as a sacrificial plate. Mold smells like mold, mycelium smells like mushrooms. You might not even smell anything and just waste a plate.


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OfflineGrenik
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Re: Check out my agar, please? [Re: karri0n]
    #27049571 - 11/20/20 10:52 PM (6 days, 2 hours ago)

Yeah, unfortunately they do not look like what I was hoping.  They do not look like mycelium. I am sorry that my advice pushed you to waste some plates and time. We have all gone through it though. I think these are probably a lost cause.

Again, sorry that I was wrong on the mycelium call. Hopefully you got some practice on technique and are still exited to try more plates!


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Onlineblack strat
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Re: Check out my agar, please? [Re: Grenik]
    #27049730 - 11/21/20 01:46 AM (5 days, 23 hours ago)

Jus opened one to sacrifice - lmfao, smelled like the wet sock you find behind the washing machine after 2 weeks.

man, do I feel like an ass....

then I opened another. it smelled like mushrooms, and mold. then another, and same thing.

is it possible there is myc in some of these, trying to compete with mold? I mean, they obviously can and do grow together.

and just for my own curiousity, what is that stuff growing out on the edge? the stuff actually growing IN the new agar, it looks like.



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Onlineblack strat
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Re: Check out my agar, please? [Re: Grenik]
    #27049735 - 11/21/20 02:02 AM (5 days, 23 hours ago)

Quote:

Grenik said:
Yeah, unfortunately they do not look like what I was hoping.  They do not look like mycelium. I am sorry that my advice pushed you to waste some plates and time. We have all gone through it though. I think these are probably a lost cause.

Again, sorry that I was wrong on the mycelium call. Hopefully you got some practice on technique and are still exited to try more plates!




whaaat dude?  hell no, do not apologize. everything is about intentions, and yours were absolutely coming from a good place.  I still very much appreciate your input.

what's got me down a little and kinda bummed, is I see TC's commenting all the time on other noob posts, throwing seasoned advice at them, definitively telling em if they got myc or not, and I still feel like I'm poking around in the dark over here man.  I really do put a decent effort into taking the best, clearest pics that I possibly can with the phone I have, because I feel like if I'm gonna ask you guys for your help, I wanna make it as easy as possible for you to check out what I got. but, ah, fuck it, that's neither here nor there. and yep, I'm sure as shit gonna keep pushing on!  what else am I gonna do hahah?


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InvisibleIdiotCircusBoy
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Re: Check out my agar, please? [Re: karri0n]
    #27049953 - 11/21/20 09:07 AM (5 days, 15 hours ago)

I've never used those ketchup cups before, but I can't imagine they are sterile or easy to make small transfers out of.  Maybe buy some plates and do a streak.  The plates aren't expensive.  I think karri0n had the right idea.  I've done and had success with shot glass approach.  My only difference is I cool the loop in receiving dish before dipping into shot glass.

 
Quote:

karri0n said:
All of the ones that it look like the pooled liquid over them are bacterial. The ones with a single drop you will need to wait probably at least a week to know if it's mycelium, mold, or bacteria. If it grows quickly it's probably mold.

Remember that spore syringes probably have quite a bit of bacteria in them, and if there is water for that bacteria to move around, it will get everywhere. Agar works for isolating mycelium from bacteria because mycelium can move, but bacteria can't, so long as there is no water and you aren't moving the plates around.

Spores take time to germinate, as well. So there will be probably 48 or so hours that nothing "good" can possibly be growing. Once they germinate, then the mycelium will start growing but still, it tends to be slower than bacteria or mold.

I never had any luck with syringe directly to agar. It's recommended  an inoculation loop, or what I use are sterile swabs.

You can take q-tips, wrap in neat little foil packages, and PC them to make sterile swabs. I did this in a jar with a standard modified myco lid.

You can also buy them from medical places or mushroom supply places, or get them from a doctor's office.

You can also rip the plastic off a bread tie, wrap it once around a toothpick, then twist the rest of it into a long handle to make an inoculation loop that you can flame.

Put a drop of spore solution onto a sterile swab and make a neat zig zag over the plate, or

Put a 1cc of spore solution into a sterile shotglass, flame your inoculation loop, dip it in the spores, and make a clean zig zag across the plate.

See these photos. When you make the zig zag, then you can separate the bacterial snots from white, organized mycelium. The bacteria was not able to move across the plate, but the mycelium was, and so I took a transfer from it.



If you wait a week or so and let the plates sit, you might see some healthy mycelium that grows away from bacterial areas. The ones that have water over the whole plate are going to turn completely into boogers most likely.




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Onlineblack strat
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Re: Check out my agar, please? [Re: IdiotCircusBoy]
    #27050005 - 11/21/20 10:00 AM (5 days, 15 hours ago)

yeah, I ordered an 8 pack of those Holy Grail jawns off amazon. says driver is out for delivery right now.

if nothing else, the ketchup cups have been a cheap way for me to just dip my toes in and start playing around with agar. I'm enjoying it. I did a print swipe with a sterile swab about 60 or so hours ago. so far none of those are showing signs of anything. which I count as a small win, because up until those, every one of my cups has been just an extremely sloppy bacterial orgy from the 70s, you know, the kind where there is just complete and utter disregard for any semblance of conservative or selective love-making -- I mean everything is fucking everything. you know that scene from the movie Caligula? yeah like that, only on a microscopic level.


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