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OfflineRollo_grows
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Registered: 09/30/20
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Agar to Agar transfer, which Plate should I choose?
    #27028150 - 11/08/20 08:43 AM (3 years, 3 months ago)

Hey everyone, I did my first Agar to Agar transfer a few days ago. I tried to choose healthy, fast growing and rhizomorphic mycelium for the transfer. But to be honest I had a hard time finding rhizomorphic and healthy growth, to me all the mycelium looked more or less tomentose. So I just wanted to know which plate you would have chosen for a transfer, just so I get more confident in identifying good looking mycelium for the next transfer. I inoculated the plates with a MS Syringe.





I would really appreciate your help :smile:


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Offlinenothanksman

Registered: 09/25/20
Posts: 76
Last seen: 6 months, 30 days
Re: Agar to Agar transfer, which Plate should I choose? [Re: Rollo_grows]
    #27028158 - 11/08/20 08:48 AM (3 years, 3 months ago)

I would go with the two in the middle. I have had success taking the edges of plates that looked like those and transferring them a couple times until I get a strong, clean looking plate. The other three look weak and the two at the top are fighting contamination.


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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,537
Loc: Taiwan
Re: Agar to Agar transfer, which Plate should I choose? [Re: nothanksman]
    #27028171 - 11/08/20 08:57 AM (3 years, 3 months ago)

is that even cubensis?


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OfflineRollo_grows
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Re: Agar to Agar transfer, which Plate should I choose? [Re: nothanksman]
    #27034717 - 11/12/20 07:45 AM (3 years, 3 months ago)

Thanks for your advice! I transferred several pieces of mycelium from dish number 3 a few days ago! I'm not going to touch number 4 anymore because I ran out of parafilm so I only wrapped it half way 😁
Did I understand you right, that there is also contamination on the first picture? The second one is obvious but I'm having trouble to find contamination on the first foto.


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OfflineRollo_grows
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Re: Agar to Agar transfer, which Plate should I choose? [Re: FriedEgg]
    #27034718 - 11/12/20 07:46 AM (3 years, 3 months ago)

It should be! I inoculated the plates with a cubensis spore syringe which I ordered from zamnesia.


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