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Offlinestar_bit
Head in the stars

Registered: 09/22/20
Posts: 14
Last seen: 3 years, 1 month
Reishi LC on Agar. What am I looking at?
    #27034351 - 11/11/20 11:32 PM (3 years, 2 months ago)

I got both the ganoderma lucidum LC and pre-poured agar plates on Etsy (I'll try making my own plates next time). The agar is MEAC, and I inoculated on 10/26 in a SAB. I didn't have an inoculation loop at the time (I have since ordered one), so I accidentally squirted too much LC on the plates. They've were wrapped in parafilm and have been stored upside down in a cabinet at 70F-75F.

I've looked on the agar envy thread and searched for other examples of reishi on agar, but I haven't seen anything quite like what I have. Mine looks almost sulfur yellow (it shows up more yellow in person), and some have whisps of mycelium (contam?) where the LC did not touch the plate.

Could y'all take a look, and let me know if I should bin these and start over? Thank you!




Edited by star_bit (11/11/20 11:46 PM)


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Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Re: Reishi LC on Agar. What am I looking at? [Re: star_bit]
    #27034680 - 11/12/20 07:23 AM (3 years, 2 months ago)

That's not how mine look at all.
In the first picture the white growth is mold.
The yellow stuff could be chicken of the woods in best case scenario. Meaning it could be a miss label from the vendor.
Have you tried contacting them?

That's a wild cloned ganoderma resinaceum.


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Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
Re: Reishi LC on Agar. What am I looking at? [Re: nosf3r4tu]
    #27034982 - 11/12/20 10:29 AM (3 years, 2 months ago)

From the looks of it the contaminant was in the LC, because the contam is so evenly spread among the culture, that it doesn't appear that a spore was introduced to the plate by bad procedure, which it would just grown from the areas it was introduced. (It's growing every place you put the LC) Ask for a new culture from the vendor before you waste more resources on it imo. Or get your money back and buy from a sponsor (Like Sporeworks), they are more reliable ime.


Edited by teknix (11/12/20 11:07 AM)


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Offlinestar_bit
Head in the stars

Registered: 09/22/20
Posts: 14
Last seen: 3 years, 1 month
Re: Reishi LC on Agar. What am I looking at? [Re: teknix]
    #27035295 - 11/12/20 01:38 PM (3 years, 2 months ago)

Quote:

nosf3r4tu said:
That's not how mine look at all.
In the first picture the white growth is mold.
The yellow stuff could be chicken of the woods in best case scenario. Meaning it could be a miss label from the vendor.
Have you tried contacting them?





Thank you for sharing your example! Yeah when I was looking for examples of ganoderma on agar everyone else's looked very thick and white. I did see some yellow mycelium but that was when it was put into fruiting conditions. I'll contact them!

Quote:

teknix said:
From the looks of it the contaminant was in the LC, because the contam is so evenly spread among the culture, that it doesn't appear that a spore was introduced to the plate by bad procedure, which it would just grown from the areas it was introduced. (It's growing every place you put the LC) Ask for a new culture from the vendor before you waste more resources on it imo. Or get your money back and buy from a sponsor (Like Sporeworks), they are more reliable ime.




Thanks for your reply! Yeah I just started with putting things on agar, but I have like 40+ other plates that have various spore swabs and LCs from other people and none of them have gotten contaminated.

They did throw in a free brown beech LC, and it was purple! My first thought was contam, but then I thought oh maybe they used agar from a purple dyed plate... but now I'm thinking it's contaminated.

I'll contact them, and I'll order some Reishi LC from sporeworks. Thanks!



Beech LC and on agar


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Invisibleteknix
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Registered: 09/16/08
Posts: 11,953
Re: Reishi LC on Agar. What am I looking at? [Re: star_bit]
    #27035382 - 11/12/20 02:43 PM (3 years, 2 months ago)

It looks really pretty whatever it is.


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Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Re: Reishi LC on Agar. What am I looking at? [Re: teknix]
    #27036335 - 11/13/20 03:40 AM (3 years, 2 months ago)

I don't know about the syringe but the white growth in those plates looks like mold. The yellow might be COTW but the clones I've made look more like dirty yellow-orange. Yours look kind of bright yellow.


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OfflineForresterM
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Registered: 02/05/13
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Re: Reishi LC on Agar. What am I looking at? [Re: nosf3r4tu]
    #27036433 - 11/13/20 06:10 AM (3 years, 2 months ago)

That's nothing but a shit ton of mold, throw the plates and the syringes away


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleSpeeker

Registered: 02/11/04
Posts: 877
Loc: Flag
Re: Reishi LC on Agar. What am I looking at? [Re: Forrester] * 1
    #27036493 - 11/13/20 07:18 AM (3 years, 2 months ago)

Kind of artistic though...

Reminds me of these...





https://www.jcvi.org/blog/holiday-art


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blog


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Offlinegabbk
Metta cultivator
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Registered: 03/06/20
Posts: 453
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Re: Reishi LC on Agar. What am I looking at? [Re: Speeker]
    #27037039 - 11/13/20 12:57 PM (3 years, 2 months ago)

Good fucking lord! Thats nasty as hell!:eek:
My Reishi looks like this:


I don't think you could really pick anything from those plates :frown: try contacting the vendor and ask for a refound!


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Offlinestar_bit
Head in the stars

Registered: 09/22/20
Posts: 14
Last seen: 3 years, 1 month
Re: Reishi LC on Agar. What am I looking at? [Re: gabbk]
    #27037231 - 11/13/20 02:54 PM (3 years, 2 months ago)

Quote:

teknix said:
It looks really pretty whatever it is.




Lol it does. I used to keep a pet fuligo septica slime mold as a pet. Maybe I have new mystery petri dish pets.



Quote:

nosf3r4tu said:
I don't know about the syringe but the white growth in those plates looks like mold. The yellow might be COTW but the clones I've made look more like dirty yellow-orange. Yours look kind of bright yellow.




Yeah in person it looks even more yellow. Like sulfur yellow. Like the color of hypholoma fasciculare fruiting bodies.

Quote:

Forrester said:
That's nothing but a shit ton of mold, throw the plates and the syringes away




Yeah I'm going to start from scratch.


Quote:

Speeker said:
Kind of artistic though...

Reminds me of these...






Pretty molds!!!


Quote:

gabbk said:
Good fucking lord! Thats nasty as hell!:eek:
My Reishi looks like this:


I don't think you could really pick anything from those plates :frown: try contacting the vendor and ask for a refound!




Yeah I gotta start over!!! I'm in contact with the vendor and he seems pretty nice and understanding, so that's good. I sent him the pictures of my agar plates and he was like wtf is that haha.

Thanks for your replies everyone!


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