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OfflineExa8yte
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Registered: 09/22/20
Posts: 11
Last seen: 10 months, 12 hours
Bacteria on Grain to Agar *DELETED*
    #27034282 - 11/11/20 10:21 PM (3 years, 3 months ago)

Post deleted by Exa8yte

Reason for deletion: .


Edited by Exa8yte (11/11/20 10:45 PM)


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Offlinescarabaeus
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Registered: 06/13/18
Posts: 1,101
Last seen: 2 years, 1 month
Re: Bacteria on Grain to Agar [Re: Exa8yte]
    #27034340 - 11/11/20 11:21 PM (3 years, 3 months ago)

Nope! Unfortunately nothing can be done despite valiant efforts from good peeps like you. Agar is just begging for any microorganism to take a foot-hold and start eating. If your bag/jar of grain is compromised even at the very least, your p-dish will fail.

Backwards engineering is not a good idea if you want to be successful. Always go spores> agar> grain.  Backwards eng. means grain to agar. Generally it never works -except if you use an antibacterial agent (gentamycin sulfate) in your agar recipe. I use gentamycin sulfate in my agar recipes when trying to clean up spores from wild collected specimens which are always dirtier than spores that you get from a vendor. So in theory at least, an antibiotic infused agar plate could accept a bacteria contaminated piece of grain for grow out.

Hope I'm not rambling. Your grain jars were most likely not sufficiently sterilized -work on that to move forward. Good luck!


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Offlinescarabaeus
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Registered: 06/13/18
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Last seen: 2 years, 1 month
Re: Bacteria on Grain to Agar [Re: scarabaeus]
    #27034374 - 11/12/20 12:02 AM (3 years, 3 months ago)

I should add that: if you have a petri dish that has good mycelial growth, and a sector of bacterial growth, common practice is to move the mycelium away from the contam, to a fresh agar plate. But my harsh response to your post was in reaction to your words 'pool of bacteria'. Pool of ugly = not so good in almost any circumstances I can think of  lol. Anyway -good luck. Respond if you like, I'll be here for a little bit if you have a question.


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OfflineExa8yte
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Registered: 09/22/20
Posts: 11
Last seen: 10 months, 12 hours
Re: Bacteria on Grain to Agar [Re: scarabaeus]
    #27034404 - 11/12/20 12:52 AM (3 years, 3 months ago)

I appreciate your reply. The reason I'm doing grain to agar is that I stupidly used all of my spore syringe going directly to grain on bags bought from etsy. Those failed so I transfered the best looking piece and used the tiny bit of left over spores to inoculate other plates(which are all showing the same pooling).

I was planning to scrape a bit of myc off the top for a transfer.. Since posting the condensation has cleared a bit and the pool may have grown. Here is a photo:



Is it possible that the 'pool' is just water? My plates were all covered in heavy condensation.


Edited by Exa8yte (11/12/20 01:00 AM)


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Offlinescarabaeus
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Registered: 06/13/18
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Last seen: 2 years, 1 month
Re: Bacteria on Grain to Agar [Re: Exa8yte]
    #27034413 - 11/12/20 01:18 AM (3 years, 3 months ago)

Hmm, maybe! Things (pics) always look weird bounced onto somebody's PC. Let her grow and see what happens. Either she will grow, or she won't and all kinds of obvious weirdness will show up instead. Good luck friend.


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