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Jbrady5555
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Registered: 12/10/19
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Dumb agar transfer questions
#26636099 - 04/29/20 06:07 AM (3 years, 9 months ago) |
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Hey folks I’ve been trying to do my due diligence but a few simple questions are eluding me as far as agar transfers go. I’ve got 10 plates knocked up from one spore syringe right now. One plate is standing out as far as growth speed goes. All of the plates have growth that doesn’t seem contamed but I really don’t know what to look for. I made 12 new agar plates yesterday, they are sitting in the pressure cooker. Am I supposed to try to knock up all my new plates with my one original plate that seems to be preforming best? Or should each original plate knock up one new plate? Should I continue to let the original plates grow after transferring out a wedge, or clean them out and prepare them for new agar? Thanks. Simple stuff I know but I’m not finding much on these procedures.
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Broken Veils
Something

Registered: 04/26/20
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Re: Dumb agar transfer questions [Re: Jbrady5555]
#26636140 - 04/29/20 06:48 AM (3 years, 9 months ago) |
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Its really hard to tell without pictures of your plates. In general, you should take transfers from the leading edge of the fastest, healthiest growth. Since your using MS you will get a wide range of growth patterns. Transfers should be small - about the size of a grain of rice or smaller. Once a transfer is made there is little need for the original plate, unless you want to let it grow out as a learning experiment. The end goal is to achieve a clean plate with desired growth characteristics to transfer to grain, WBS, etc...good luck!
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: Dumb agar transfer questions [Re: Broken Veils]
#26636162 - 04/29/20 06:59 AM (3 years, 9 months ago) |
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I keep the original plates as the next ones grow out just incase something goes wrong you have that original to go back to. Develop a label system to track everything using a system of letters and numbers to keep track of the family tree
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Rumpleforeskin
Scientist new to mycology



Registered: 07/05/20
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Re: Dumb agar transfer questions [Re: Broken Veils]
#27018502 - 11/02/20 11:12 PM (3 years, 3 months ago) |
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Quote:
Broken Veils said: Its really hard to tell without pictures of your plates. In general, you should take transfers from the leading edge of the fastest, healthiest growth. Since your using MS you will get a wide range of growth patterns. Transfers should be small - about the size of a grain of rice or smaller. Once a transfer is made there is little need for the original plate, unless you want to let it grow out as a learning experiment. The end goal is to achieve a clean plate with desired growth characteristics to transfer to grain, WBS, etc...good luck!
As long as the source plates are clean, wouldn't it be worth keeping them for A2G transfers? as time progresses and the transfers become more aggressive, the results should continually improve until you start to take tissue cultures, no? I'm just thinking about keeping the older ones and using them as long as they are clean. As the transfers continue, turf any older generations that are in excess of the need for additional jars/bags. I accidentally bought smaller plates (47mm). If the plate is clean and mostly populated, any recommendations for how much to a jar or potentially a bag (I think I prefer a g2g for the bag).....?
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Broken Veils
Something


Registered: 04/26/20
Posts: 41
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Keeping the plates is not advised in my opinion...Each time you take a transfer from a dish you are creating a potential for contams. To me its not worth the risk. A contam might not have time to express itself before completing another transfer to grain. Use the entire plate to inoculate jars or bags. The amount/size of the agar wedge you use is up to you. In general, the more wedges of agar you use the faster the colonization. You could knock up 4 to 5 jars per plate.
Also - Taking a transfer does not speed up colonization times.
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