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Offlinestigma13
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First time using agar, checking in & next steps
    #27033567 - 11/11/20 02:43 PM (3 years, 3 months ago)

Hi all!

I'm sure this is searchable, but since I'm very new to this I thought I'd get some input from you guys.

All of them was from MS syringes and it has now been 15 days after inoculation.

1.) Do the below look healthy to you?
2.) Any recommendations from where I should transfer? How many first transfers should I do (ballpark figure :smile:)?
3.) There's some fluffiness going on, is that normal? 
4.) All of the look quite different, especially the Cyanescens, is it generally a slower/different grower? It doesn't look nearly as nice as some of the other Cyan posts  I've seen. :crazy:


Ps. Cubes B+


Ps. Tampanensis


Pan. Cyanescens


Thanks, any advise would really be appreciated!


Edited by stigma13 (11/11/20 03:13 PM)


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Offlineconformity
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Re: First time using agar, checking in & next steps [Re: stigma13]
    #27033866 - 11/11/20 05:28 PM (3 years, 3 months ago)

Im new to agar myself but, I would say those are way to grown out to say any one spot is good or bad. They look good overall so take a very small transfer from the best organized spot and watch it closely.


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Offlinestigma13
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Re: First time using agar, checking in & next steps [Re: conformity]
    #27034450 - 11/12/20 02:25 AM (3 years, 3 months ago)

Thanks! I think my technique was a bit shoddy with the syringe - used much more than a drop or two. Should I do a couple of transfers from each dish e.g 3 and and up with 12 new dishes?


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Offlineconformity
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Re: First time using agar, checking in & next steps [Re: stigma13]
    #27035641 - 11/12/20 05:11 PM (3 years, 3 months ago)

That's what I would do.


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