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PeterPumpkinEater
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Registered: 08/25/19
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about using less water in my agar recipe, due to pooling innoculant.
#27032740 - 11/11/20 03:55 AM (3 years, 2 months ago) |
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I squirted some innoculant onto my dishes but I see the excess moisture from the innoculant seems to be preventing the mycelium from rapidly growing further, almost trapping them in a bubble lick coating of moisture: I can see however the spores have germinated, but that's about as far as it goes. It appears to be excess moisture
I was wondering if I can reduce the moisture content of my agar recipe from say 500 ml to 400 ml, would that do something..
anyone have experience with this?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Loc: Milky way
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Re: about using less water in my agar recipe, due to pooling innoculant. [Re: PeterPumpkinEater] 1
#27032781 - 11/11/20 05:06 AM (3 years, 2 months ago) |
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Solving the problem the wrong way. Don't just squirt inoculant onto a plate. Use an inoculation loop and do it the right way
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One of Us
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Re: about using less water in my agar recipe, due to pooling innoculant. [Re: bodhisatta]
#27032849 - 11/11/20 06:34 AM (3 years, 2 months ago) |
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When I inoculate with a syringe, I of course use as little as possible, firstly. Then I tilt the plate to get the droplet to roll in a zigzag pattern. Most importantly to your question, I leave my inoculated plates unwrapped in my sab for about 24 hours to get rid of excess moisture.
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PeterPumpkinEater
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Registered: 08/25/19
Posts: 171
Last seen: 1 month, 4 hours
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Re: about using less water in my agar recipe, due to pooling innoculant. [Re: One of Us]
#27033368 - 11/11/20 12:37 PM (3 years, 2 months ago) |
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Hi, these plates of mine are still unwrapped after like a week. Sadly only one is left uncontammed.
My eyes have finally been opened to what sterile technique is... Quite happy to say I'm pretty sure I will make a success of it this time, slowly but surely...
Bodhisatta, how do you use an inoculation loop with a spore syringe, or a jar with spore water... I just read prior to your post about using an inoculation loop - I had used a simple blunt edged butter knife to scrape off spores off the print :rolls eyes:
Anyways, thank you for the advice, I was very clumsy in controlling the plunger of the syringe, I shall certainly take care from now on to only drop a drop or so... mistakes go a long way to edify us 
Thanks for the response guys.
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Edited by PeterPumpkinEater (11/11/20 12:45 PM)
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PeterPumpkinEater
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Registered: 08/25/19
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Last seen: 1 month, 4 hours
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Re: about using less water in my agar recipe, due to pooling innoculant. [Re: bodhisatta]
#27036940 - 11/13/20 12:02 PM (3 years, 2 months ago) |
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Quote:
bodhisatta said: Solving the problem the wrong way. Don't just squirt inoculant onto a plate. Use an inoculation loop and do it the right way
58 000 + posts. Holy shit. OK! <3 awesome. When did you start "working" here? Are you one of the founding fathers of shroomery or something. And in fact, you are who they call "Bod"?
Epic man...! Epic.
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