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SporesAU
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Registered: 11/10/20
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How am I stuffing up making Agar
#27031808 - 11/10/20 03:01 PM (3 years, 2 months ago) |
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Hey Guys, first time posting and very new so go easy.
I'm trying to make an agar mix to pour into dishes and just cant get it to mix properly. The recipes I've found seems pretty simple and I'm following the instructions but I just cant get the it to mix properly. I always end up with solid AGAR at the bottom and LME liquid at the top. Here's the amounts and method I've used.
3g Telephone Agar Agar Powder 3g LME 200ML Distilled Water
1. Add the Agar and LME together 2. Pour water (boiled or not) in altogether/slowly and mix
I'm not sure what I'm missing at it seems pretty simple but just cant get it mixed properly. Thanks guys any help is appreciated.
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One of Us
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Re: How am I stuffing up making Agar [Re: SporesAU]
#27031813 - 11/10/20 03:09 PM (3 years, 2 months ago) |
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Are you doing petris or pasty plates? I don't have any experience with the latter, but when I PC my agar before pouring, most of the time I need to give the media bottle a good shake after sterilizing to get everything mixed.
I am assuming you are doing pasty style plates though, right? Have you tried bringing the mixture (not just the water) to a boil before pouring?
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SporesAU
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Registered: 11/10/20
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Re: How am I stuffing up making Agar [Re: One of Us]
#27031827 - 11/10/20 03:18 PM (3 years, 2 months ago) |
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I'm doing petris I believe. Just using a regular Ball jar as the container as I don't have a media bottle. Should I just mix it best I can and after sterilizing give it a good shake before pouring?
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One of Us
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Re: How am I stuffing up making Agar [Re: SporesAU]
#27031860 - 11/10/20 03:44 PM (3 years, 2 months ago) |
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I think that should fix the problem. Best to do it right after pc while it's hottest, just be careful. Just tighten the lid and leave the foil in place, shake, then leave to cool a bit before pouring.
If you are using the two piece metal lids, I think it would be best to use the disc part upside-down so it doesn't seal. Some agar may leak a tad, I'm not sure, but it should be fine if you pour immediately when cooled to pouring temp, rather than store. If the lid has a filter, than that would be even less ideal but still might work.
If you use the plastic lids, especially the no-leak lids, than that is perfect.
It may be best to bring the mixture to a boil before sterilizing, and make sure everything is dissolved properly before putting in the PC.
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SporesAU
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Registered: 11/10/20
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Re: How am I stuffing up making Agar [Re: One of Us]
#27031911 - 11/10/20 04:12 PM (3 years, 2 months ago) |
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I have ball jars with the 2 part lids, they have a GE hole and an injection port but flipping them and adding tape over the hole should work. I'll try that method but I think part of the issue is it just not dissolving not matter how much I mix it. I've tried boiling aswell but it just kinda foamed over and didn't work well at all. So should I just add all ingredients together with boiling water, mix as well as I can, PC, shake and pour?
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Inthepit
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Registered: 08/20/19
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Re: How am I stuffing up making Agar [Re: SporesAU]
#27031954 - 11/10/20 04:40 PM (3 years, 2 months ago) |
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