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Offlinebucksid8
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Registered: 03/25/19
Posts: 15
Loc: Santa Cruz Mountains
Last seen: 10 months, 15 days
Mycelium randomly stops growing
    #27031516 - 11/10/20 12:01 PM (3 years, 2 months ago)

I've recently been having the worst luck with agar and am hoping someone here could give me some advice about what I'm doing wrong. First things first I've been cloning mycelium to new agar dishes in an attempt to "run" from contamination. Took me quite a few petri dishes but I finally got one that hasn't turned green (yay!) only problem is, about 3-4 days ago the mycelium stopped growing and now has a wet matted look to it



This leads to my second question: why the **** do my agar dishes have so much condensation on the top lid??  :confused:  I've tried placing a hot mug of tea on top of them which helps for about 5 minutes until they recondense. I run a heater in my room 24/7 to keep the temps up as it's winter here and can get quite cold at night. The room typically stays between 70-75F with the heater going. Also I wrapped the plates with micropore tape to keep contaminants out



Now my third question is: is it normal for the bottom of the dish to look yellowish as pictured above? Could that possibly have something to do with the mycelium growth rate?

Any help would be greatly appreciated. I've been getting so frustrated lately just trying to get ONE good agar plate to make into a LC.


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 9 hours
Re: Mycelium randomly stops growing [Re: bucksid8]
    #27031584 - 11/10/20 12:46 PM (3 years, 2 months ago)

Contam city:thumbdown:

These are no good for LC.

Take the growth earlier, make more transfers


Edited by smalltalk_canceled (11/10/20 12:47 PM)


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Invisibletijuanataximan
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I'm a teapot

Registered: 07/09/20
Posts: 32
Loc: cananananada Flag
Re: Mycelium randomly stops growing [Re: smalltalk_canceled]
    #27031607 - 11/10/20 01:02 PM (3 years, 2 months ago)

Definitely doesn't look right.

I'm in the works of figuring out the condensation problem myself - I've read that if you leave your (unwrapped) plates in your SAB for a 24h period or in front of your flow hood for an 8h period it will clear up the condensation by evaporation. Personally I'm going to attempt lower temperature pours (somewhere below 115f/46c) to try to stop my condensation problem.

What recipe are you using for your agar btw?


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Offlinebucksid8
Shroomer
Male

Registered: 03/25/19
Posts: 15
Loc: Santa Cruz Mountains
Last seen: 10 months, 15 days
Re: Mycelium randomly stops growing [Re: tijuanataximan]
    #27031620 - 11/10/20 01:08 PM (3 years, 2 months ago)

Quote:

tijuanataximan said:
Definitely doesn't look right.

I'm in the works of figuring out the condensation problem myself - I've read that if you leave your (unwrapped) plates in your SAB for a 24h period or in front of your flow hood for an 8h period it will clear up the condensation by evaporation. Personally I'm going to attempt lower temperature pours (somewhere below 115f/46c) to try to stop my condensation problem.

What recipe are you using for your agar btw?




I just ordered them online. It's MEA


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Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
Re: Mycelium randomly stops growing [Re: bucksid8]
    #27031655 - 11/10/20 01:31 PM (3 years, 2 months ago)

That looks like Candida, wut you been eating? :lol:


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