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OfflineMortyC137
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Need help ID these contams. Can’t seem to figure out what I’m doing wrong.
    #27031408 - 11/10/20 10:40 AM (3 years, 2 months ago)

Been staying away from g2g after my cat had to toss out 60+ qt jars due to contams. Since then he has been doing strict agar work and using one plate per jar with no g2g action whatsoever. Recently been losing jars left and right to different contams. No idea what it could be, possibly contam hidden in a seemingly good plate? If anyone has insight or has gone through this please share! Here’s some pics of what’s going on. TIA!



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InvisibleWrightii
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137]
    #27031494 - 11/10/20 11:47 AM (3 years, 2 months ago)

I have no experience growing on popcorn, but a first thing you could try is preparing some jars, and not innoculating them. If they still get contams you know sterilization is not adequate. If they stay clean, the problem is prob. in the agar or the sterile technique.
If the sterilization is not adequate maybe look at other grains/teks (proper soaking makes a lot of difference imy), try if you can score some autoclave tape for reassurance. Also check the rubber ring on your autoclave, it might be up for replacement

This might help for the agar:
https://www.shroomery.org/forums/showflat.php/Number/22020260#22020260
I have to say that the contam has quite a distinct colour though, making it less likely to hide on agar.
What I would do if sterilization is adequate:
Check sterile procedure, make sure to rub everything down with desinfectant before use and properly flame sterilize your blade.
Give your working area (that is the whole room, not just the SAB or FH) a thourough cleaning, wiping extensively with desinfectant.
Don't open the jars inside, this will spread the contam everywhere.

This is just my 5 cents, but maybe some of it can help :smile:

Cheers,
-Wii


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The Official Hericium Thread


Edited by Wrightii (11/10/20 11:49 AM)


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OfflineMortyC137
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: Wrightii] * 1
    #27043931 - 11/17/20 01:59 PM (3 years, 2 months ago)

Thanks a lot mate! I got replacement o rings for my autoclave. I agree that this contam would be very hard to hide on agar. Great recommendations I appreciate you!


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"If you wish to forget anything on the spot, make a note that this thing is to be remembered."
-Edgar Allan Poe :ducklol:


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OfflineLogicaL ChaosM
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137]
    #27044032 - 11/17/20 03:11 PM (3 years, 2 months ago)

Could be from your grain (popcorn). What sort of PC cycle do u give them?


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OfflineMortyC137
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: LogicaL Chaos] * 1
    #27055970 - 11/24/20 06:27 PM (3 years, 2 months ago)

40 min at 15 psi on a 23qt presto


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"If you wish to forget anything on the spot, make a note that this thing is to be remembered."
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InvisibleWall.E
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137] * 1
    #27055980 - 11/24/20 06:33 PM (3 years, 2 months ago)

Quote:

MortyC137 said:
40 min at 15 psi on a 23qt presto




This is it. Definitely not long enough for those jars


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Invisiblemushhead
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: Wall.E] * 1
    #27056000 - 11/24/20 06:45 PM (3 years, 2 months ago)

Quote:

MortyC137 said:
40 min at 15 psi on a 23qt presto




M8 you need 1hr 45min to 2hrs to sterilize proper like.


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OfflineLogicaL ChaosM
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: mushhead]
    #27056007 - 11/24/20 06:51 PM (3 years, 2 months ago)

Yup, definitely not enough PCing time. Theres the issue right there.


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OfflineMortyC137
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: LogicaL Chaos]
    #27056636 - 11/25/20 06:15 AM (3 years, 2 months ago)

DUUUUUDE I’ve been getting by for over a YEAR pc like this omg. Thank you all for your advice and input! I was losing about 3 jars for every dozen I pc. Alright so just to be clear, I was pc just the popcorn for 40 min. Then after jarring for another 40 min. Should I bump up both times? Or just the jar pc cycle?


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"If you wish to forget anything on the spot, make a note that this thing is to be remembered."
-Edgar Allan Poe :ducklol:


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InvisibleWall.E
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137]
    #27056647 - 11/25/20 06:27 AM (3 years, 2 months ago)

Wait, what? You're pressure cooking your popcorn? Like, you're putting it in with water and pressure cooking for 40 min? That seems excessive, but that's just me.

The times we are referring to are your jar cycles. You'll want at least 90 minutes but gonna be safer with a 2 hour cook at 15 psi


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Invisiblemushhead
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137]
    #27056999 - 11/25/20 11:32 AM (3 years, 2 months ago)

Quote:

MortyC137 said:
DUUUUUDE I’ve been getting by for over a YEAR pc like this omg. Thank you all for your advice and input! I was losing about 3 jars for every dozen I pc. Alright so just to be clear, I was pc just the popcorn for 40 min. Then after jarring for another 40 min. Should I bump up both times? Or just the jar pc cycle?



Bruh just clean the grain, soak it to hydrate, and then PC for 1hr 45min.
Your jars will be perfect every time...
Washing grain is easy, just wash it out with cold tap water.
Soak it in hot tap water for 12-24hrs (depends on the grain)
Drain, load jars, PC.
Simple.


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Offlinesmalltalk_canceled
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: mushhead]
    #27057019 - 11/25/20 11:45 AM (3 years, 2 months ago)

Hi. I've grown on corn for a year.

1 How long are you sterilizing your corn, 2 how big are the jars
3 Are you using additives like coff

Edit: 40 mins!?

Recommendations:

2h pc cycle from when it hits pressure

Pcing corns in water for 20 mins is a great prep, that's not a problem I think


Edited by smalltalk_canceled (11/25/20 11:47 AM)


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InvisibleLemgrub
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: smalltalk_canceled] * 1
    #27057168 - 11/25/20 01:52 PM (3 years, 2 months ago)

I'd suggest using a different grain too. I saw a good tek for millet recently, and I personally recommend rye grass seed, stupid simple prep.


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OfflineMortyC137
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: smalltalk_canceled]
    #27062028 - 11/28/20 06:37 PM (3 years, 1 month ago)

Quote:

smalltalk_canceled said:
Hi. I've grown on corn for a year.

1 How long are you sterilizing your corn, 2 how big are the jars
3 Are you using additives like coff

Edit: 40 mins!?

Recommendations:

2h pc cycle from when it hits pressure

Pcing corns in water for 20 mins is a great prep, that's not a problem I think




Hello there thanks for chiming in on the corn! Yeah the corn prep I do about 30-40 I’ll dial it down to 20 min, I didn’t think the extra time would make much of a difference.

Sorry if anyone got confused there I know we don’t pc other grains like this however I found this method on a “ quick popcorn tek” I am bumping up my jar cycle to no less than 90 min. Just got an outdoor propane burner so I can go crazy with the pc.

I have used Rye, Wbs, and whole oats previously, popcorn is the most affordable and accessible IMO. I dislike the prep for other grains it’s a pain in the ass, corn is pretty simple. Thanks for helping me out y’all are the GOAT 🐐


--------------------
"If you wish to forget anything on the spot, make a note that this thing is to be remembered."
-Edgar Allan Poe :ducklol:


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Offlinesmalltalk_canceled
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Re: Need help ID these contams. Can’t seem to figure out what I’m doing wrong. [Re: MortyC137]
    #27062064 - 11/28/20 07:00 PM (3 years, 1 month ago)

Corn is full of contams in my experience

Do full 2h


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