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Offlineteslacann
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Contamination? *DELETED*
    #27031195 - 11/10/20 08:00 AM (3 years, 3 months ago)

Post deleted by teslacann

Reason for deletion: n/a


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Offlinetiptrippy
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Re: Contamination? [Re: teslacann] * 1
    #27031197 - 11/10/20 08:01 AM (3 years, 3 months ago)

Any pink or red colors means contamination. Sorry about your luck πŸ˜”


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Offlinekanemush
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Re: Contamination? [Re: teslacann] * 1
    #27031198 - 11/10/20 08:02 AM (3 years, 3 months ago)

Quote:

teslacann said:
Hi everyone!
I was hoping that someone would be able to help me out. I'm giving growing mushrooms a bash for the first time and I am a little unsure if this contamination or if this is just how it should be. Thanks in advance for your help!






Looks pretty fishy to me, but let some others chime in.


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Offlineteslacann
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Re: Contamination? *DELETED* [Re: kanemush]
    #27031203 - 11/10/20 08:08 AM (3 years, 3 months ago)

Post deleted by teslacann

Reason for deletion: n/a


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Invisibletijuanataximan
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Re: Contamination? [Re: teslacann] * 1
    #27031209 - 11/10/20 08:18 AM (3 years, 3 months ago)

If you mean you tried to colonize your grains in that tub than yeah that very well could be the issue. Bags or jars are pressure cooked to kill off contams and you certainly can't pressure cook that container lol

If I wasn't sure I'd crack the lid and smell it to see if it smells off. When in doubt throw it out.


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Offlineteslacann
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Re: Contamination? *DELETED* [Re: tijuanataximan]
    #27031212 - 11/10/20 08:21 AM (3 years, 3 months ago)

Post deleted by teslacann

Reason for deletion: n/a


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OfflinePearl
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Re: Contamination? [Re: teslacann] * 1
    #27031263 - 11/10/20 09:04 AM (3 years, 3 months ago)

Always best to do your own so you know it's done properly.

Is there an injection port on the lid somewhere?

If you opened the lid to inoculate, it wouldn't matter if the grains were sterile prior or not... (assuming the inoculation was done outside of a SAB/flowhood setup)


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OfflineSingularFusion
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Re: Contamination? [Re: Pearl] * 1
    #27031293 - 11/10/20 09:36 AM (3 years, 3 months ago)

sometimes this is what happens when you noc up grains with syringes

they are not expected to be 100% clean, that's why people work with agar first and then put that to grain after a couple of transfers, ensuring a healthy culture

when you roll the dice with mss to grain sometimes you crap out

hopefully you get a better roll on your other bag

all that also assumes all other factors are on point, I dunno where you are at with things generally

but yep syringes are certainly not clean starting material for grain inoculation, even under the very best of circumstances


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OfflineNiftySlix
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Re: Contamination? [Re: SingularFusion] * 2
    #27031342 - 11/10/20 10:02 AM (3 years, 3 months ago)

Quote:

Nef said:
sometimes this is what happens when you noc up grains with syringes

they are not expected to be 100% clean, that's why people work with agar first and then put that to grain after a couple of transfers, ensuring a healthy culture

when you roll the dice with mss to grain sometimes you crap out

hopefully you get a better roll on your other bag

all that also assumes all other factors are on point, I dunno where you are at with things generally

but yep syringes are certainly not clean starting material for grain inoculation, even under the very best of circumstances




Certainly an unclean syringe would be a problem starting with grain or anything else, but why would a spore syringe be an issue for starting on grain specifically? I've got 36 grain spawn jars and 3 grain spawn bags  colonizing right meow and I haven't had any issues thus far (aside from the bags not showing any sign of life for a whole week after the jars I inoculated the same day and colonizing SIGNIFICANTLY slower even after showing growth). Not in any way saying you're wrong, just wondering, this is my first time working with grain so I've got plenty of learning to do :smile:


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OfflineLogicaL ChaosM
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Re: Contamination? [Re: teslacann] * 1
    #27031441 - 11/10/20 11:01 AM (3 years, 3 months ago)

Looks like green mold to me, probably from the spore syringe u used.


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Offlineteslacann
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Re: Contamination? *DELETED* [Re: LogicaL Chaos]
    #27031452 - 11/10/20 11:05 AM (3 years, 3 months ago)

Post deleted by teslacann

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OfflineLogicaL ChaosM
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Re: Contamination? [Re: teslacann] * 1
    #27031453 - 11/10/20 11:07 AM (3 years, 3 months ago)

Hmmm. In that case, its probably the grain itself. Also, its best to use agar->grain and avoid putting spores directly on grains.


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OfflineSingularFusion
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Re: Contamination? [Re: NiftySlix] * 1
    #27032443 - 11/10/20 10:45 PM (3 years, 3 months ago)

Quote:

NiftySlix said:
Quote:

Nef said:
sometimes this is what happens when you noc up grains with syringes

they are not expected to be 100% clean, that's why people work with agar first and then put that to grain after a couple of transfers, ensuring a healthy culture

when you roll the dice with mss to grain sometimes you crap out

hopefully you get a better roll on your other bag

all that also assumes all other factors are on point, I dunno where you are at with things generally

but yep syringes are certainly not clean starting material for grain inoculation, even under the very best of circumstances




Certainly an unclean syringe would be a problem starting with grain or anything else, but why would a spore syringe be an issue for starting on grain specifically? I've got 36 grain spawn jars and 3 grain spawn bags  colonizing right meow and I haven't had any issues thus far (aside from the bags not showing any sign of life for a whole week after the jars I inoculated the same day and colonizing SIGNIFICANTLY slower even after showing growth). Not in any way saying you're wrong, just wondering, this is my first time working with grain so I've got plenty of learning to do :smile:





I very well might be wrong man, I haven't been at this that long myself, but it is just not the done thing if you are talking about the optimal

people noc grains with mss all the time, 60% of the time, it works every time lol... it's just a pretty hit and miss affair

agar gives you confidence that the culture is good. I mean would you wanna g2g something you did up with a syringe? Even if I had 100% confidence in my ability to see the contam in a jar (I don't), I can't see 100% of the actual jar(the core), so my diagnosis is only partly effective even in the best case scenario. Agar adds several layers of inspection to the source material before I even noc a jar

all just my humble opinion eh :rockon:


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Offlineteslacann
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Re: Contamination? *DELETED* [Re: SingularFusion]
    #27033385 - 11/11/20 12:47 PM (3 years, 3 months ago)

Post deleted by teslacann

Reason for deletion: n/a


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OfflineSingularFusion
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Re: Contamination? [Re: teslacann] * 1
    #27034147 - 11/11/20 08:17 PM (3 years, 3 months ago)

one of these two first, depending on your confidence

https://www.shroomery.org/forums/showflat.php/Number/19208976

https://www.shroomery.org/forums/showflat.php/Number/21922023


then this one

https://www.shroomery.org/forums/showflat.php/Number/18430998


there are obviously many teks out there and I'm sure they are probably every bit as valid, these are just the ones I always remember to link to

good luck


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OfflineNiftySlix
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Re: Contamination? [Re: SingularFusion] * 1
    #27034359 - 11/11/20 11:42 PM (3 years, 3 months ago)

Oh I definitely agree going agar first is going to give you better results because of the contam control and the ability to isolate preferred genetics. I’m just starting a new grow right now and it’s my first time working with agar but I wasn’t gonna use >ALL< my spores for agar so I started a few agar samples for the different strains I’m working with from a drop or two from each mss. For the remaining 9.99CCs from each I got about 40 some odd GSJs going and a few LCs (total not like for each of the 5 strains lol). Time and spores, waste not want not amirite?


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Offlineteslacann
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Re: Contamination? *DELETED* [Re: LogicaL Chaos] * 1
    #27044037 - 11/17/20 03:13 PM (3 years, 2 months ago)

Post deleted by teslacann

Reason for deletion: n/a


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OfflineLogicaL ChaosM
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Re: Contamination? [Re: teslacann] * 1
    #27044042 - 11/17/20 03:18 PM (3 years, 2 months ago)

Yeah, using spores on grain is bad practice because spores are not clean. The only thing Ive found thus far to "decontaiminate" spores is to mix spores with a very dilute water-Lysol solution. The Lysol is very potent so only one drop is needed for a syringe and doesnt destroy the mushroom spores. Only drawback is that its an unnatural chemical that ends up in your grow.


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Offlineteslacann
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Re: Contamination? [Re: LogicaL Chaos]
    #27080480 - 12/09/20 01:06 PM (3 years, 2 months ago)

Would this work if you were to put those spores onto agar? I've got a problem with the water the spores are in having some kind of contamination


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OfflineLogicaL ChaosM
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Re: Contamination? [Re: teslacann]
    #27080500 - 12/09/20 01:22 PM (3 years, 2 months ago)

I dont see why it wouldnt work. Worth a try :shrug:


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