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Offlinepitriot
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How is this Agar ?
    #27030293 - 11/09/20 01:54 PM (4 years, 2 months ago)

I cloned a cube (Jedi) by putting a small piece on agar. This is what I got. Would you transfer or just put to grain as is?
If you would transfer does it make a difference from which part I would take a wedge (it all looks pretty uniform to me)?
If put to grain would you first make LC or LI?



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Offlinemad_dingo
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Re: How is this Agar ? [Re: pitriot]
    #27030318 - 11/09/20 02:03 PM (4 years, 2 months ago)

If you start with mushroom tissue it's always a good idea to make at least another transfer (or two or three) to agar before going to grain. It might look clean, but there's a good chance of contams hiding beneath your mycel. It's safer after transfering small clean pieces from the edge of growth to fresh agar.

Also you don't need to wait till the whole dish is colonized, even if you intend to put the whole puck to grain.

Speaking of going to grain. If it's clean you can just dump the whole agar puck into the grain jar (following proper aseptic procedures of course), no need to make liquid whatever to do that. Only if you want to inoculate many jars at once it starts to get easier going with liquid inoculant.

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Offlinepitriot
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Re: How is this Agar ? [Re: mad_dingo]
    #27030430 - 11/09/20 03:44 PM (4 years, 2 months ago)

Thanks for you reply.
I was going to post this a few days ago but it colonized quicker than expected, less than 6 days.
I will do more transfers. Is there any place on the agar you would transfer from rather than other places? (They all look pretty much the same to my noobie eyes.)

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Offlinemad_dingo
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Re: How is this Agar ? [Re: pitriot]
    #27030923 - 11/09/20 11:48 PM (4 years, 2 months ago)

I would follow the lore as put forward on this site and pick from the edge of the colony, prefering rhizomorphic ('ropey') growth.

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