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InvisibleMateja
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Re: LC Testing + Grow Log [Re: mushhead]
    #27027466 - 11/07/20 07:35 PM (3 years, 3 months ago)

Quote:

mushhead said:
:tryingnottodie:



:whathesaid:


--------------------
Cakes inside Water Tub


Edited by Mateja (11/07/20 07:36 PM)


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InvisibleJosex
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Registered: 11/13/15
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Re: LC Testing + Grow Log [Re: Mateja]
    #27029693 - 11/09/20 07:24 AM (3 years, 3 months ago)

Thermophilic bacteria? I don't know if you're purposely or inadvertently glossing over the fact that vegetative bacteria is not the issue, these are easily killed. We're trying to destroy endospores produced by bacteria of the bacillus genus. :shrug:

Endospores can and will survive a PC cycle. The degree in which this happens will depend on different variables such as type of grain, quaility of the grain, level of hydration, duration of the cycle, etc.

The surviving endospores will produce bacillus bacteria and these are responsible for the so called "wet spot" and that sweet smell in grain spawn reminiscent of rotting apples. It is also the most common contaminant in grain spawn, followed by bad inoculant and technique. How most people get this backwards these days? Beats me.

I could tell you lots of personal anecdotes that made it obvious for me that bacterial spores are very capable of surviving a PC cycle. But I'm but an individual and for all you know I could be biased to the brim. Think instead of the remarkable measures some spawn producers take in order to achieve true sterilization of the grain to supply to mushroom farms and all the anecdotal evidence from home cultivators around the world throughout the years. It's always been known that endospores can survive a PC cycle, but somehow that knowledge has gradually been swept under the rug like it never happened. Beats me too.

You're relying on what microbiology has to say about how vegetative organisms with metabolic activity can be killed, but they talk nothing about non metabolic bacterial spores that are protected from the lethal action of moist heat by things such as the starches, oils and proteins that are present in the grain.

Also this,
Quote:

bit2ez said:
I am impressed by your ingenuity in temping your spawn jars. However, you are faced with having to explain what is physically being observed. Though flying in the face of basic microbiology, an adjustment has to be made to procedure and thinking. Your jars show bacterial and mold contamination, your LC doesn't and your technique for inoculating has a high success rate. The easiest thing to adjust is adding time to your sterilization cycle. You have hours of leeway until the grains start to caramelize / maillard.




You seem to want to do real science but when presented with something like this you'd rather look the other way. Real science is not biased like that.


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InvisibleD3_Myc
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Registered: 05/06/18
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Re: LC Testing + Grow Log [Re: Josex]
    #27029722 - 11/09/20 07:47 AM (3 years, 3 months ago)

Josex, in your anecdotal experience is there any grain that reins supreme over the others in regards the above aforementioned? The wheat I get seems to be ever persistent with bacteria, usually not terrible, just a hint of sweet smell and rarely a damp grain or two. I stopped soaking over night and just boil till I can chop into it with a fingernail. I don’t know if cutting out the soak has helped or compounded the issue.


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InvisibleJosex
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Re: LC Testing + Grow Log [Re: D3_Myc]
    #27029735 - 11/09/20 07:58 AM (3 years, 3 months ago)

Quote:

D3monic said:
Josex, in your anecdotal experience is there any grain that reins supreme over the others in regards the above aforementioned? The wheat I get seems to be ever persistent with bacteria, usually not terrible, just a hint of sweet smell and rarely a damp grain or two. I stopped soaking over night and just boil till I can chop into it with a fingernail. I don’t know if cutting out the soak has helped or compounded the issue.




Have you tried seed? Wbs, millet, etc.
These are inherently cleaner. About soaking, all I can say is that I made the switch from boilling only to soaking and boilling, and will never go back to boilling without soaking first. Your experience may vary I guess.


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InvisibleStipe-n CapMDiscord
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Re: LC Testing + Grow Log [Re: Josex]
    #27029741 - 11/09/20 08:02 AM (3 years, 3 months ago)

So you still boil millet afterwards to evaporate? Have you done soak only no boil? I want to be able to prep bulk grains without energy and heat.


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InvisibleJosex
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Re: LC Testing + Grow Log [Re: Stipe-n Cap]
    #27029748 - 11/09/20 08:05 AM (3 years, 3 months ago)

The only way I have been able to prep millet right is soaking with cold tap water, straining real quick until it stops dripping and then funnel it into jars wet, no boil at any step. But there are lots of people who do different preps, gotta find what works for you.


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InvisibleD3_Myc
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Re: LC Testing + Grow Log [Re: Josex]
    #27029773 - 11/09/20 08:29 AM (3 years, 3 months ago)

I just bought a bag of white millet to try but haven’t ran it yet. It’s by the same producer of my wheat. Runs me about $4 more a bag which isn’t bad.

So cold soak (overnight?) and then strain and jar. I’ll give it a go next round.


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InvisiblemushboyMDiscord
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Re: LC Testing + Grow Log [Re: D3_Myc]
    #27029776 - 11/09/20 08:31 AM (3 years, 3 months ago)

Just like burdced:awesomenod:


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InvisibleJosex
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Re: LC Testing + Grow Log [Re: D3_Myc]
    #27029791 - 11/09/20 08:41 AM (3 years, 3 months ago)

Quote:

D3monic said:
I just bought a bag of white millet to try but haven’t ran it yet. It’s by the same producer of my wheat. Runs me about $4 more a bag which isn’t bad.

So cold soak (overnight?) and then strain and jar. I’ll give it a go next round.




Just 4$ more than wheat? Fml I get 25 kilos of this stuff for 18 euros whereas I can get 40 kilos of (shit) wheat for 10.


Yea I like to soak for 24 hours but first I rinse the millet like 3 times to remove all the floaters.


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InvisibleStipe-n CapMDiscord
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Re: LC Testing + Grow Log [Re: Josex]
    #27029797 - 11/09/20 08:45 AM (3 years, 3 months ago)

Quote:

Josex said:
The only way I have been able to prep millet right is soaking with cold tap water, straining real quick until it stops dripping and then funnel it into jars wet, no boil at any step.




Perfect, thanks. This is exactly how I'd like to do it.


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OfflineA.k.aM
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Re: LC Testing + Grow Log [Re: Stipe-n Cap]
    #27029807 - 11/09/20 08:52 AM (3 years, 3 months ago)

I recently switched to a wbs that’s mostly millet, and now that I’m getting to the end of the bag it’s basically pure millet.


It doesn’t seem like it until you go to spawn a tub but a quart of millet is way more spawn than other stuff. It makes sense because it’s so small it packs together super tight. I’ve also had problems with bacteria recently so I think it takes slightly longer to completely sterilize too.


--------------------
LAGM2020


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InvisibleStipe-n CapMDiscord
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Re: LC Testing + Grow Log [Re: A.k.a]
    #27029819 - 11/09/20 09:03 AM (3 years, 3 months ago)

I'll be running 4lb bags at 3.5 to 4 hours in the 75x.


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