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GTEED
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What do you think this is?
#27027740 - 11/07/20 11:14 PM (3 years, 3 months ago) |
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Well, I have used the search function and read a few posts trying to understand what exactly I have going on. One of my guesses is this is cobweb. I could be wrong I am nowhere near an expert yet. Here's something a little different that I couldn't find anything about while using the search function. So I used 2 different rye berries prep teks one was a quick no soak tek and the other was a soak tek of about 22 hours. I mixed them up so can really only tell what tek was used by the looks of the rye (more plump with the soak), but the odd thing is what ever is growing in these jars actually grew inside of a jar I did not inoculate yet. It was an extra jar I just put the left over hydrated berries in and let it sit. A few days ago I noticed it had this same sort of growth in it just not very much yet. If this is not any sort of contamination then my next guess would be somehow my rye berries are creating this mycelium looking stuff. It is growing in small patches that shouldn't have any growth because I used agar to inoculate and its random growth on the opposite area in a few of the jars. Possible growth of some sort. Does anyone have any experience with this? Suggesttions would be appreciated. Thanks.
sorry for the long runon sentence, but its late and am tired 
Heres some photos to help.
Edited by GTEED (11/07/20 11:53 PM)
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FriedEgg



Registered: 09/22/14
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Loc: Taiwan
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Re: What do you think this is? [Re: GTEED]
#27027751 - 11/07/20 11:19 PM (3 years, 3 months ago) |
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it's not cobweb. you will never see cobweb in your entire life. you have a higher chance of getting struck by lightning.
i see some tomentose mushroom mycelium in all those jars and a spot of mold where that green spot is. you zoomed in on it so i'm sure you know where it is. how did you sterilize your jars? if you hydrate grain and put it in a jar, it will grow mold unless you sterilize it soon. and did you use a proper still air box? or did you inoculate in open air? from agar? spore syringe?
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GTEED
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Re: What do you think this is? [Re: FriedEgg]
#27027780 - 11/07/20 11:58 PM (3 years, 3 months ago) |
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Yes, I went from spore print to agar in a SAB. only did one transfer of agar to agar. Then soaked the rye then dried for 1.5 hours then straight to PC. The little patches of growth I am questioning to me it is growing in random places. If I used an agar wedge wouldn't it slowly move as one growth instead of like 4 little patches and 1 big growth where the agar is?
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FriedEgg



Registered: 09/22/14
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Re: What do you think this is? [Re: GTEED]
#27027784 - 11/08/20 12:05 AM (3 years, 3 months ago) |
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if you shook the jar after dropping in your agar wedge then it can have multiple inoculation points. how long did you PC for and at what PSI? it should be 90-120 minutes at 15 PSI.
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GTEED
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Re: What do you think this is? [Re: FriedEgg]
#27029370 - 11/08/20 11:07 PM (3 years, 3 months ago) |
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No shaking after droping in agar. I vent for 10 mins once i see steam coming out then start a timer on my phone for 1hr 30 mins and i keep it above 15 psi the whole way through. I have a few more pictures that pretty much prove in my opinion that the rye berries are sprouting somehow.
These pictures below are grain that were soaked and boiled for 10 mins then put into a jar but not PCed. The same growth is in some of my jars I ended up Pcing and putting the Agar into. Does anyone know why the berries are sprouting? too wet?
My room humidity is around 55-50% RH
Edited by GTEED (11/08/20 11:08 PM)
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FriedEgg



Registered: 09/22/14
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Re: What do you think this is? [Re: GTEED]
#27029410 - 11/08/20 11:48 PM (3 years, 3 months ago) |
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that is strange. so some of the jars have that white growth even after you PC'd and before you inoculated them? try to PC for 2.5 hours. what do your lids look like?
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GTEED
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Re: What do you think this is? [Re: FriedEgg]
#27029427 - 11/09/20 12:15 AM (3 years, 3 months ago) |
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Yea. this is how its looking after PCed and inoculated.

(the Above pic)^^^This is a jar that I droped a piece of agar on that did not end up doing much from the agar. But i have all of this random growth that i believe is the same stuff that poped out of the single seed.
(Bottom picture) heres a different view of the single seed that has the weird growth.
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GTEED
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Re: What do you think this is? [Re: GTEED]
#27029428 - 11/09/20 12:16 AM (3 years, 3 months ago) |
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I am wondering if somehow some cobweb/mold spore landed on that single piece of grain lol
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GTEED
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Re: What do you think this is? [Re: GTEED]
#27029430 - 11/09/20 12:19 AM (3 years, 3 months ago) |
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Here are the lids
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FriedEgg



Registered: 09/22/14
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Re: What do you think this is? [Re: GTEED]
#27030833 - 11/09/20 09:45 PM (3 years, 3 months ago) |
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i don't know why you'd see growth in multiple sections of your jar if you only dropped 1 agar wedge on the top without shaking it.
i think you need to run a test. sterilize a grain jar and put it on a shelf for 2 weeks without opening it or inoculating it. if it grows anything, your sterilization cycle is not sufficient or your lids/filters are not working properly.
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