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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27005113 - 10/26/20 05:50 PM (3 years, 3 months ago)

Yes. The cold slows down the growth. The colder the better as long as you don't freeze. Cube myc can't hardly do a thing near freezing. But if you freeze it it'll die.

Slants are best for long time storage but plates will last a good long while if kept properly.


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InvisibleNecroMyce
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Registered: 05/12/13
Posts: 747
Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27005134 - 10/26/20 06:04 PM (3 years, 3 months ago)

Sweet. I was planning on doing slants eventually, but not until I get my master plates done,
Knock up a couple 5qt size grain Growbag, get a few tubs going and see how the fruits do, I plan on cloning a fruit from That first run And then using that plate to make a slant as time goes on, probably sometime next year.


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OfflinePhony Phone

Registered: 09/19/20
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27005873 - 10/27/20 08:19 AM (3 years, 3 months ago)

46-48 F (8-9 celsius) is fine fridge temp for storage?
OT: Realized the plates i had were unsterilized :facepalm: might explain the satellite contams around the myc. Now must be redone over again

:crazy2:


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: Phony Phone] * 1
    #27011191 - 10/29/20 09:55 PM (3 years, 3 months ago)

I hate to make a thread carry on, but I feel as there is a lot of people learning here. And everyone can learn together, no reason to make a new thread.

So I started PR, CR, AA+, Burma, Malabar all from spore inoculation right on to agar, I made about 3-4 plates for each strain. And of course I had contams, so I transferred those plates. And look at that, most are pretty clean and have rhizoporn growth. Going to let these go a few more days and then make another transfer. Not sure if that’s the best idea, but my plan is to get a few fully grown plates for inoculations. Basically have them in the fridge for whenever I’m ready, a nice clean master culture, once I only have one little piece of the agar left, or one plate left, I’ll use that plate to make more plates and it’s a never ending cycle.

I also clones a few large fruits from my 2nd flush. I plan on letting those go a little longer and doing some transfers and once that’s clean, fruiting those.

Here is some pictures. Not really sure when the best time to transfer is. I’ve read the earlier the better. But I don’t know. I’m also curious if these plates grow out and are perfectly clean, why not just start inoculating with it, and use the last piece to make another master culture. Hmmmm



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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27011620 - 10/30/20 08:01 AM (3 years, 3 months ago)

Looks like you're doing wonderfully.

When to transfer just depends on the plate and what's going on. Sometimes you want to hurry and snatch something up before something goes fucky and sometimes you want to wait a minute and watch things grow out so you can see what's up.


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27012033 - 10/30/20 12:31 PM (3 years, 3 months ago)

But in theory unless we are isolating strains and mainly going for inoculation plates, as long as it’s clean we are good! The idea mainly is to have clean culture to inoculate with? If nothing goes wrong in a plate, let it grow and either store it in a fridge to halt growth, or inoculate some bags? I was gonna drop a decent sized cut from these plates to a 5quart spawn bag to skip the grain to grain process. Am I following?


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OfflinePhony Phone

Registered: 09/19/20
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27012365 - 10/30/20 03:47 PM (3 years, 3 months ago)

A kinda trivial question: how do you make your agar? Its pristine


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: Phony Phone] * 1
    #27012563 - 10/30/20 06:02 PM (3 years, 3 months ago)

PastyWhyte write ups

Here’s a good link,

But here’s the recipe I used D3’s perfect transfers

I know this link above is a link for how to make transfers, but if you read it, it’ll show his agar recipe, a lot of people use Potato flakes and yeast and all that, but I went simple and used just agar and light malt extract. I suggest using the food coloring too because it makes the mycelium nice a white and easier to see contams, and it looks badass! I feel like a science expert, and your family and friends that watch you do agar work or see your progression will think the same lol!


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27016713 - 11/02/20 03:56 AM (3 years, 3 months ago)

Its me again.

Im uploading 2 pics showing mycelium that seems to be "eaten away" at one point. It's a patch with fuzzier mycelium growth over it. You can see the rhziomorphs stalling right behind the fuzzier growth.

What could it be?



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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: Phony Phone]
    #27017286 - 11/02/20 11:12 AM (3 years, 3 months ago)

Could be ten million different things. Most likely though are the following

A: Contams lurking in the culture and fucking shit up.

B: Just different sets of genetics figuring their shit out and showing different morphology.

C: Some combination of the above.

I'd guess it's just different genetics but it's not easy to tell sometimes. I'd suggest taking transfers and seeing how they do. Looking pretty fuckin good though.


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27018570 - 11/03/20 12:55 AM (3 years, 3 months ago)



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OfflineMr. Mushie

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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27027515 - 11/07/20 08:07 PM (3 years, 3 months ago)

Man I'm bummed none of my 290 plates all transfer #3 even look close to this good.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27027674 - 11/07/20 09:56 PM (3 years, 3 months ago)

290? What the fuck? Why?


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OfflinePhony Phone

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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27027926 - 11/08/20 04:31 AM (3 years, 3 months ago)

pics?


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OfflineGrinchGrower
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Re: Agar transfer, avoiding isolation [Re: Phony Phone]
    #27028614 - 11/08/20 01:24 PM (3 years, 3 months ago)

Maybe he means 290 total in his lifetime of agar work?


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OfflineMr. Mushie

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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27028728 - 11/08/20 02:28 PM (3 years, 3 months ago)

I understand, it's probably overkill.

The most challenging part of cultivation for me currently is grain prep and agar work. I figured I'm going to lose some jars to contamination along the way so why not make an insane amount of plates so that I can inoculate 100+ jars.



Here's 170 plates all T3 started from two MS syringes. Some have gone to grains, most have not.


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InvisibleStipe-n CapMDiscord
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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27028736 - 11/08/20 02:33 PM (3 years, 3 months ago)

Quote:

Mr. Mushie said:
Here's 170 plates all T3 started from two MS syringes. Some have gone to grains, most have not.




Agar is meant to clean and isolate. Just a tip to consider for the future, if you don't intend to use those plates for creating spawn then having that many is a waste of resources. You aren't helping anything by running that many.

When cleaning just transfer to one or two plates at a time. It doesn't help at all to have that many, even if you're just using in for practice it's still a waste.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27028746 - 11/08/20 02:38 PM (3 years, 3 months ago)

:whathesaid:

The smartest and most cost and energy effective way to go about that is to make one or two plates of each variety and transfer until you know it's clean. Then you take the clean plate and noc up a few jars. You can make as many or as few jars per dish as you want but i wouldn't recommend more than ten jars per dish. You then let these jars grow out and G2G into more jars. You can turn ten jars into 200 jars in no time. Some cultivators utilized the same principle but choose to expand myc via LC instead. Either way works fine if done properly.

You are wasting time and plates. Look at the wall of plates in my sig. I've been there. That's how i know.


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OfflineMr. Mushie

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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27028770 - 11/08/20 02:54 PM (3 years, 3 months ago)

Thank you both for the response.

My worry is that I'm going to mess up grain prep (too dry, or too wet) and if I only had two plates, that i'd lose all my jars due to noob errors and id be back at the starting line.

I figured making a crazy amount of plates while I practice grain prep with my first 50# bag wouldn't be a terrible idea.

Thanks again for the knowledge verum and p9hu7.

I'm simply worried about a small mistake that ends up ruining 6 weeks of work.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27028778 - 11/08/20 02:58 PM (3 years, 3 months ago)

It's understandable. Still way way overkill. Ten plates would be more than enough. Good news is, if all those plates are good. You can throw extras in the fridge and use as needed. Be sure to take proper precautions if you go the fridge route though.

Grain prep is easy. What type of grain are you using?


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