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Offlinethebigcheese
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Registered: 08/09/20
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Last seen: 2 years, 11 months
Mycelium Growth Rings
    #27027559 - 11/07/20 08:34 PM (3 years, 2 months ago)

I was getting very strong rhizo growth from some transfers, but then the rhizo growth stopped and I started to get fuzzy rings of growth at the leading edge. Has anyone experienced this?





It has been cold during the night where I am, and I'm wondering if the temp swings (down to 40F at night) may have caused this pattern.

Any insight into why the rhizo growth stopped?


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Offlineshroomrookie
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Registered: 07/24/05
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Re: Mycelium Growth Rings [Re: thebigcheese]
    #27027626 - 11/07/20 09:17 PM (3 years, 2 months ago)

I've had that happen but I though it to be some type of contamination. IDK. I don't think those plates of mine did well


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Offlinethebigcheese
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Re: Mycelium Growth Rings [Re: shroomrookie]
    #27027653 - 11/07/20 09:43 PM (3 years, 2 months ago)

I don't think it's contam bc the plates are sealed with parafilm and I never opened them b/t transferring and seeing this growth. Also the fuzzy growth is spreading radially from the leading edge.

If it was contam I would expect that I would've seen it during the initial rhizo growth period (~2 weeks) and not be growing in a nice radial pattern from the transfer site leading edge.

But also just noob so irdk.


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Offlineshroomrookie
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Re: Mycelium Growth Rings [Re: thebigcheese]
    #27027693 - 11/07/20 10:16 PM (3 years, 2 months ago)

I'm not sure either. Mine also spread in the same radial pattern and that threw me on thinking contamination but I made several transfers from the obvious good growth areas. Those transfers were fine but the plate kinda petered out.


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Offlinetanuki
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Re: Mycelium Growth Rings [Re: thebigcheese]
    #27027703 - 11/07/20 10:26 PM (3 years, 2 months ago)

I agree with your theory about the temperature fluctuations causing the growth rings.

I have my agar plates sitting on a seedling mat and I started getting those when I was toggling it on/off for cycles.


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Offlinethebigcheese
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Re: Mycelium Growth Rings [Re: tanuki]
    #27028295 - 11/08/20 10:05 AM (3 years, 2 months ago)

Interesting, thanks for sharing.

I'm going to experiment with an insulated cooler with a heat bomb to try to keep the mycelium incubating at a more constant temp around 75-80F and see if that produces better growth.


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