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Another_Hobby
Stranger
Registered: 09/23/20
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Agar Contam Double Check
#27023419 - 11/05/20 02:53 PM (3 years, 3 months ago) |
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1st time pouring agar and inoculating with GT from a spore syringe. This is just practice at this point, going to keep on making plates and inoculating/transferring until I get the hang of it. Looking forward to the day after enough experimentation where I can get some nice rhizomatic growth. Might try cloning from some BRF cakes that I have going as well to see how that goes.
Pretty sure this is cobweb mold:
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Phony Phone

Registered: 09/19/20
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How fast did it grow to that point?
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PrincessRosalina
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Re: Agar Contam Double Check [Re: Phony Phone]
#27023665 - 11/05/20 05:12 PM (3 years, 3 months ago) |
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Doesn't really look like cobweb from the picture. Germination plates never really look great. Take some transfers and go from there.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Germ plates can be funny, for sure. I think that's fucked though.
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FunnelMind
Student



Registered: 09/22/20
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Last seen: 2 years, 1 month
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I am also a noob when it comes to agar and I have share the same concerns. I have 8 different glad rounds of agar that I inoculated in a SAB, 11 days ago. I have 3 different types of mushrooms and all of them look similar which leads me to believe they are all mycelium, and not cob web mold. Especially since I was extra sterile and all growth is coming from the spores I dropped in. Those little black specs that you see are the spores I inoculated with. growth has been coming outwards from them. This is just the agar round with the most growth, and I am beginning to think that maybe I just put too many spores in each one and I have never seen spores germinate on agar before in real life. If anyone could answer both of our questions, it would also put my mind at ease. i am hoping I can transfer to grain soon.
-------------------- Thanks, FunnelMind
Edited by FunnelMind (11/05/20 07:23 PM)
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IdiotCircusBoy
Human Person



Registered: 09/22/20
Posts: 294
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Did you just pour the one plate??
-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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verum subsequentis
seeker of truth



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Re: Agar Contam Double Check [Re: FunnelMind]
#27023941 - 11/05/20 08:17 PM (3 years, 3 months ago) |
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It's hard to tell from the pic but it looks like you may have cube myc.
You can't open a plate and take a picture without contaminating the plate unless you do it correctly. How'd you go about that? Even holding your camera above an open plate will likely contam it.
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Another_Hobby
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Re: Agar Contam Double Check [Re: Phony Phone]
#27025017 - 11/06/20 12:58 PM (3 years, 3 months ago) |
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Quote:
Phony Phone said: How fast did it grow to that point?
7 days to get to that point.
Edited by Another_Hobby (11/06/20 12:59 PM)
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Another_Hobby
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Quote:
PrincessRosalina said: Doesn't really look like cobweb from the picture. Germination plates never really look great. Take some transfers and go from there.
I have been looking for inoculation pics from a spore syringe, but the ones I see are transfers and they look far better. I was thinking about transferring it to see that process. I am also questioning the agar I made, first time doing that so who knows if I didn't accidently mess up the recipe.
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Another_Hobby
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Quote:
verum subsequentis said: Germ plates can be funny, for sure. I think that's fucked though.
I haven't seen the term 'germ plate' used before. Is that specific to the act of putting spores onto agar versus agar to agar transfers? The latter being the ones I see with beautiful rhizomatic growth?
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FunnelMind
Student



Registered: 09/22/20
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Last seen: 2 years, 1 month
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As far as my post, I have multiple plates going. This one has the most growth. I thought it was myceluim but wasn't sure, since I have never seen it germinate this way before. I knew I was risking contaminating it, but I wanted to ask others about it before I invest more time, money and energy going forward with all my plates. They all look similar and growth started from the spore sites (where the black specs are). The black specs are definitely spores. They looked like that when I dropped them in from the print.
I opened the plate in a sterile SAB. I tried to be quick with it. I only had it open for about 3 or 5 seconds to take one picture and then closed it up again. I haven't opened any of the other plates though. Thank you for your answer and sorry for kind of butting into this conversation, but I felt the same way as the original poster and wanted other opinions on mine as well.
-------------------- Thanks, FunnelMind
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IdiotCircusBoy
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Re: Agar Contam Double Check [Re: FunnelMind]
#27025491 - 11/06/20 06:07 PM (3 years, 3 months ago) |
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FunnelMind - You should create a new post for this. IMO
-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 856
Loc: California, baby!
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Quote:
Another_Hobby said: 1st time pouring agar and inoculating with GT from a spore syringe. This is just practice at this point, going to keep on making plates and inoculating/transferring until I get the hang of it. Looking forward to the day after enough experimentation where I can get some nice rhizomatic growth. Might try cloning from some BRF cakes that I have going as well to see how that goes.
Pretty sure this is cobweb mold:

So what does it taste like? You can use a clean inoculation loop to carefully pull out maybe 1/3 of the growth. Sniff it, and if it doesn't smell like mold, try a careful taste. Mushroom mycelium tastes exactly like mushrooms. It's a strong enough flavor that even a small sample should be recognizable.
If it tastes like moldy bread: You have mold.
Another option: if you have a microscope, put the sample under the microscope and see what it looks like at 100x to 200x. Molds are heavily branched and even look kind of like a pile of twigs. Mycelium looks smoother.
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CocaineBuffet
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Re: Agar Contam Double Check [Re: sonoramo] 1
#27025569 - 11/06/20 06:51 PM (3 years, 3 months ago) |
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Quote:
sonoramo said:
Quote:
Another_Hobby said: 1st time pouring agar and inoculating with GT from a spore syringe. This is just practice at this point, going to keep on making plates and inoculating/transferring until I get the hang of it. Looking forward to the day after enough experimentation where I can get some nice rhizomatic growth. Might try cloning from some BRF cakes that I have going as well to see how that goes.
Pretty sure this is cobweb mold:

So what does it taste like? You can use a clean inoculation loop to carefully pull out maybe 1/3 of the growth. Sniff it, and if it doesn't smell like mold, try a careful taste. Mushroom mycelium tastes exactly like mushrooms. It's a strong enough flavor that even a small sample should be recognizable.

I would not advise anyone to taste their petri dishes to determine if something is mold or not... What if it is mold??
I don't know about other users but I have been able to get through this hobby by using my eyeballs and agar.
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CaptainParanoid
Stranger Danger



Registered: 08/06/20
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Still a noob myself. But this looks like normal growth. Give it time.
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 856
Loc: California, baby!
Last seen: 1 hour, 57 minutes
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Quote:
CocaineBuffet said:
Quote:
sonoramo said:
Quote:
Another_Hobby said: 1st time pouring agar and inoculating with GT from a spore syringe. This is just practice at this point, going to keep on making plates and inoculating/transferring until I get the hang of it. Looking forward to the day after enough experimentation where I can get some nice rhizomatic growth. Might try cloning from some BRF cakes that I have going as well to see how that goes.
Pretty sure this is cobweb mold:

So what does it taste like? You can use a clean inoculation loop to carefully pull out maybe 1/3 of the growth. Sniff it, and if it doesn't smell like mold, try a careful taste. Mushroom mycelium tastes exactly like mushrooms. It's a strong enough flavor that even a small sample should be recognizable.

I would not advise anyone to taste their petri dishes to determine if something is mold or not... What if it is mold??
I don't know about other users but I have been able to get through this hobby by using my eyeballs and agar.
I didn't mean to suggest sticking your tongue in the dish and lapping it up. I meant, collect a grain-size sample with a sterile loop. A rice-grain-sized sample of fluffy white growth is very small and your chances of harm are zero. You can taste the mushroomy flavor from even a very small amount of mycelium. Most molds (not all, though!) taste really bad. No need to get a mouthful of it to differentiate from other fluffy white growths. Spit it out if it tastes bad in any way. Which you would have done anyway.
If you have coronavirus or an impaired sense of smell for any other reason, obviously don't do this!
Molds are most dangerous when spores are inhaled.
Check this out.
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Another_Hobby
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Re: Agar Contam Double Check [Re: sonoramo]
#27027493 - 11/07/20 07:54 PM (3 years, 3 months ago) |
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I transferred a piece to a new petri dish and will wait and see what happens. Every experience is a learning experience.
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