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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 5 days
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Question about .2 micron bags
#27026644 - 11/07/20 11:32 AM (3 years, 2 months ago) |
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Hello,
I made a previous post about this in the contam thread but thought this might be better suited for its own thread, please delete if not allowed.
I recently bought 50 lb rye berries along with a bunch of compost and casing determined to start something up. I was on a budget and got extremely flustered when I realized that I need filter discs, along with silicone/rtv to make the lids. My rye berries had already been soaking overnight and I was starting to boil them in batches (I have 12 pounds soaking altogether!
Than I realized I have a bunch of .2 and .5 micron bags that I’ve never used. I’ve already read the .5 micron bags are no good so that’s out of the question. But I have some .2 bags and I’m currently loading them up with rye berries.
The next question: can I pressure cook these without the bags melting? I read they explode if you seal them and pressure cook, so what’s the correct way to pc while keeping sterile and not letting any moisture in the bag? I have a vac seal, I also have clips that came with the bag.
From their, can I inject my spores directly into the bag and slap some micropore tape over the injection spot?
Any and all help is appreciated, thank you
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bit2ez
Stranger

Registered: 11/06/20
Posts: 25
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Search for Jar lid teks, there's more out there than just filter discs and RTV is 5-7 bucks a tube.
The .2 micron bags are preferred for spawn, but if you are colonizing in a "clean", still environment you will have success with the .5 micron if you must use them. I wipe the bag with bleach, inject, and put a piece of tape over it. Don't use micro pore, it's weak and the bags already have a filter.
You don't have to get all the air out of the bag when folding, just a gentle, firm press will do. I'm sure there are videos on the internet of people doing this.
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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 5 days
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Re: Question about .2 micron bags [Re: bit2ez]
#27027179 - 11/07/20 04:39 PM (3 years, 2 months ago) |
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I’m confused about one part of this. You seal the bag after pc? Would that lead to contams?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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You can seal before or after. I don't preseal. I just get the gussets lined up, press most of the air out of the bag, fold nicely and load up the pc. I then download them with iso cleaned gloved hands onto the iso cleaned flow table with the flow running and noc them up. I seal them after noccing.
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
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Seal the bags before PC if you like to cook a few rounds before inoculating them and skip sealing if you inoculate straight after PC. If you seal them, you can just keep them on the shelf longer without contamination, but It can work unsealed too if you don't break the vacuum in the bags.
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mcnugget
Stranger


Registered: 06/22/13
Posts: 260
Last seen: 1 month, 17 days
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Re: Question about .2 micron bags [Re: bit2ez]
#27027915 - 11/08/20 04:11 AM (3 years, 2 months ago) |
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Damn I thought .5 microns wouldn't be suitable for spawn. I've been looking at a box of them sitting on my shelf so I may give it a shot in a few days, make 4 bags and just see how it goes. Getting sick of dealing with so many jars.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Question about .2 micron bags [Re: mcnugget]
#27028968 - 11/08/20 05:22 PM (3 years, 2 months ago) |
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Many people use .5 for spawn. I don't.
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