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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27039343 - 11/14/20 07:00 PM (3 years, 4 months ago)

Should I be concerned about the fluffy growth at 6 o'clock? Should I make another transfer?



The brf cake on the left is APEU and the one on the right is ESS.



The grain jars that I inoculated are slow to recover. Its been 7 days and I have maybe half the jars showing recovery and growth while the other jars are showing no growth at all.

Edited by BrownBear (11/14/20 07:02 PM)

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Invisibleeatyualive
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27040921 - 11/15/20 04:53 PM (3 years, 4 months ago)

Ess can be a little tricky. You may have to give it more fae than other tubs.


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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: eatyualive]
    #27044186 - 11/17/20 04:27 PM (3 years, 4 months ago)

Thanks for the tip, eat.

None of the oat jars are showing any signs of contamination. This is the best jar I have so far. The others are showing little to no growth at all.



I also made some rice cakes. I inoculated some of these with lc and others with agar cultures.



This is a culture on nutritional yeast agar. Recipe is 500 ml water, 15 grams of agar powder and 10 grams NY.


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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27047664 - 11/19/20 04:36 PM (3 years, 4 months ago)

So I went a head and tried my no prep oat tek. I used 112 grams of dry whole oats and 90 grams of water per pint jar.

Jars just after adding oats and water.



I gave them all a good shake after putting the lids on. I pc'd them at 15 psi for 90 minutes. I let them cool for a day in my lab. I then turned on my flowhood and let it run for a few minutes before opening the pc.

Jars right out of the pc.




The jars after a good shake.



Next round I might add just a little less water. What do you all think?

Edited by BrownBear (11/19/20 04:39 PM)

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OfflineShu
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Re: BrownBear's Grow Log Adventure [Re: Sold Out Online]
    #27047809 - 11/19/20 06:02 PM (3 years, 4 months ago)

Quote:

cdnpsychonaught said:
I think your pressure cooker needs to run at 15 PSI.



With cookers that use a rocker to maintain pressure it is more likely that the gauge is incorrect than the rocker has lost weight.

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: Shu]
    #27052700 - 11/22/20 06:01 PM (3 years, 4 months ago)

I shook this rice jar 2 days ago. Its just about ready. Probably be ready by tomorrow night at the rate it has been going just from my observation today. I have eleven others right behind it.



I have 6 sterilized oat pint jars that I have waiting to be g2g'd with this rice jar.

I sterilized 24 qts of oats today. I loaded them 3 qts per bag. I have 2 pint lc's that should be fully colonized in another day or so. I will inoculate the eight bags with one of those lc's.



I think I made a mistake with my first lc to oat jars. I did not shake after inoculation and the lc was too much liquid and not enough culture. It took a good 12 to 13 days for most of the jars to come around. I shook the few of them that were the best of them 2 days ago. Imo, this one and the other few are recovering rather nicely. How does this oat jar look to  you? If this one looks healthy than I would like to use it for g2g after full colonization. At this rate of recovery, I'm thinking another 2 days to fully colonize.


Edited by BrownBear (11/23/20 04:25 PM)

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27054242 - 11/23/20 04:35 PM (3 years, 4 months ago)

Out of the 8 pre sealed bags of grain that I sterilized yesterday only one popped during the 3 hour run at 15 psi. It popped because it was the only one that I did not  roll very tight. There was some air left in it after sealing. I might try my vacuum sealer next time and see how that works.



These are my dozen 1/4 pint rice jars. One is fully colonized and the rest are not too far behind  the first jar I am going to g2g to 6 pints of oats. The rest of them I was thinking g2g to 3 qt grain bags with one per bag.


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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27055815 - 11/24/20 04:34 PM (3 years, 4 months ago)

I have lost all but 2 of my dozen ziploc rice containers. It was most likely a combination of over hydration and poor technique. They all suffered from bacteria contamination.



The two that are healthy look like this.



This is the second 1/4 pint to fully colonize.



I really like these quarter pint jars of rice. I inoculated 6 pints of oats with the first 1/4 pint to fully colonize. Less than 24 hours later they are showing great recovery speed.



My next step with these 1/4 pint rice jars is to make thick, muddy slurry with them and use them to inoculate grain bags.

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27058717 - 11/26/20 12:39 PM (3 years, 3 months ago)

24 hrs


48 hrs



72 hours


96 hrs



120 hrs


144 hrs


168 hrs


192 hrs


216 hrs





Edited by BrownBear (12/02/20 04:49 PM)

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27061280 - 11/28/20 08:48 AM (3 years, 3 months ago)

I made surry with 100 ml of water and one 1/4 pint of rice spawn. I used 1 slurry per two 3qt bags of oats. Less than 24 hours after inoculation and I am seeing good recovery.



I wasn't exactly satisfied with the consistency of the slurry as I wanted it to be a little more thick than it turned out. So the next batch I went with 60 ml of water per one 1/4 pint of rice spawn rather than the 100 ml of water I used the first batch. I am really satisfied with the 60 ml slurry. There is no excess water and the consistency is that of loose oatmeal.

This morning I decided to use 1 slurry per 3qt bag of oats.


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InvisibleD3_Myc
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27061305 - 11/28/20 09:09 AM (3 years, 3 months ago)

Hey, sorry I missed this thread BB. Glad to be tagging along now though. I tried the ziplock containers and the lids didn’t want to thread on well after a pc run. Even had one pop off when I picked it up. Decided not to use them again.

I got one them adjustable window screens and it fits perfectly over the kitchen sink. Fairly cheap too though 10 jars worth you wont be able to spread out 1 grain thick. Just requires a few stirs to dry em all

https://www.homedepot.com/p/W-B-Marvin-21-37-in-W-x-18-in-H-Clear-Wood-Frame-Adjustable-Window-Screen-AWS1837/202088352

I need to try my hand at bags, do you use the standard amount of water?

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: D3_Myc] * 1
    #27061360 - 11/28/20 09:55 AM (3 years, 3 months ago)

When I switched to the bags I changed my grain prep. I add my oats to a large stock pot. Fill until the water is 2 inches above the grain line. Bring the water to a boil (stirring occasionally). Turn off heat. Put lid on pot and let it sit for 20 minutes or so. When oats meet the water line. I dump them out on a screen. After water stops dripping, I spread the oats out and leave them until they cool. I don't even let them completely dry. If you test the oats though they are just slightly under cooked. I then load bags, weigh them and then adjust to make them all even. I use 1 1/2 qts of dry oats per bag.

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: D3_Myc]
    #27062122 - 11/28/20 07:59 PM (3 years, 3 months ago)

Quote:

D3monic said:
Hey, sorry I missed this thread BB. Glad to be tagging along now though. I tried the ziplock containers and the lids didn’t want to thread on well after a pc run. Even had one pop off when I picked it up. Decided not to use them again.

I got one them adjustable window screens and it fits perfectly over the kitchen sink. Fairly cheap too though 10 jars worth you wont be able to spread out 1 grain thick. Just requires a few stirs to dry em all

https://www.homedepot.com/p/W-B-Marvin-21-37-in-W-x-18-in-H-Clear-Wood-Frame-Adjustable-Window-Screen-AWS1837/202088352

I need to try my hand at bags, do you use the standard amount of water?




Hi, D3monic. Thanks for joining my grow log.

When you ran the ziploc containers through the pc, did you put the lids on a bit loose and wrap the entire thing in aluminum foil? Thats what I do and I have yet to observe any warping.

Kitchen screens work really well for laying out grain. I used to own one of those adjustable window screens. They work well also.

I have never been a fan of using quart jars. They sometimes end up cracking on the bottom. However, I would need too many pint jars for what I want to accomplish. Bags just seem right. Plus storing bags take up a lot less space than storing jars. They also make biodegradable bags. I can shoot you a :pm: of where I get them from, if you like?

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InvisibleD3_Myc
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27062200 - 11/28/20 09:31 PM (3 years, 3 months ago)

I have a 50 pack I ordered I haven’t tried yet. Been meaning to give them a go. Just switched to millet, any different prep there?

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: D3_Myc]
    #27062405 - 11/29/20 03:35 AM (3 years, 3 months ago)

I've recently tried to source millet locally but haven't had any luck. Its been so long since I have prepped millet that I honestly do not have a preferred prep for it. From what I remember is that it is a lot like rice in that it is not very forgiving and can turn to shit rather easily.

Edited by BrownBear (11/30/20 07:26 AM)

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27064914 - 11/30/20 03:52 PM (3 years, 3 months ago)

I will be making 12 transfers tonight from these 3 plates. I dyed my agar for the first time. I have seen a plethora of colored plates on the boards. They make looking at the mycelium so much more interesting. I can also understand its function as well as its appeal.





This is one of the bags that I inoculated with a 1/4 pint rice slurry 2 days ago. Recovery is real nice. The slurry significantly increases the points of inoculation. This has me really excited.


Edited by BrownBear (11/30/20 05:41 PM)

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27066803 - 12/01/20 05:18 PM (3 years, 3 months ago)

The lc recovers so nicely on the rice. I saw recovery in under 24 hours. This pic is day two. I have also observed recovery in 24 hours with the rice slurry.



These bags are one in the same. First pic was taken yesterday. The second pic is 24 hours later. These bags are 3 days old today.



These are the eight 3qt grain bags inoculated the same way on the same day.



Day 4 of the rice slurry to oats bag.


Edited by BrownBear (12/02/20 04:41 PM)

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OfflineBrownBear
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Re: BrownBear's Grow Log Adventure [Re: BrownBear] * 1
    #27070404 - 12/03/20 05:20 PM (3 years, 3 months ago)

This is a TOC t3 from germination. I plan on putting this in a lc. I poured 40 plates last night. I have a bunch of transfers that I need to make tomorrow from various cultures.



The slurry oat bags should all, but one, finish up in the next 24 hours. 6 day's to full colonization is pretty good, isn't it? The slower bag is probably about 2-3 days behind the rest. One bag I had to get rid of though. I saw the tiniest spec of green on an oat kernel. Sucks but was most likely caused by poor inoculation technique and/or just the fact that I cannot seal the bags directly in front of the flowhood.



I spawned 6 cups of rice spawn to 12 cups of coir 5 days ago. I topped it with 4 cups of coir at spawning. Is it common for a tub spawned 1:2 ratio to fully colonize in 5 days? It has even colonized around 20% of the top layer of coir.


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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
    #27070740 - 12/03/20 09:05 PM (3 years, 3 months ago)

Nice, you will find shaking that slurry and covering every single grain will help your time decrease. Just takes a little practice.

6 is damn good but you can get 3-4 days. Just a simple getting used to how it works. And i prefer my slurries on the muddy side so it sticks to all the grain. I dont remember if you made a slightly more liquid mix. If so, use a little less water for more of a sludge kinda consistency.


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OfflineShroomoisseur
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Re: BrownBear's Grow Log Adventure [Re: eatyualive]
    #27070847 - 12/03/20 10:04 PM (3 years, 3 months ago)

I’m excited for you dude! Can’t wait to see the fruits of your labor, I’m just getting started with agar myself! Good luck!!!

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