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Mateja


Registered: 07/14/16
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Re: Some notes about LCs [Re: sonoramo]
#27020045 - 11/03/20 07:05 PM (3 years, 3 months ago) |
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Quote:
sonoramo said:
Quote:
Mateah said:
... I made 5 liquid cultures in varying strengths of ELME at 0.1%, 0.2%, 0.4%, 0.8%, and 1.5% strength. I inoculated all LC´s with the same clone using The Biopsy Method by Josex.
Very, very nice work! Can you elaborate, ELME is powdered "Extra Light Malt Extract"? Is it different from "LME" or what Amazon calls "Organic Light Dried Malt Extract DME - Maltoferm 10001"? Are the percentages relative weight of powder and water, or something else?
Thanks!
The malt I have is a thick amber syrup, I've never worked with the powdered one. And yes percentages are by weight.
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Nurkurzda23
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Re: Some notes about LCs [Re: Mateja]
#27022538 - 11/05/20 02:52 AM (3 years, 3 months ago) |
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Hm i think i have some contamination
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PitcherCrab
Crescent Fresh



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It looks that way. Growth on the top of the LC is typically not a good sign.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Nurkurzda23
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can I store the LC syringes in the refrigerator for a longer period of time?
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PitcherCrab
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Do you guys leave you LCs on stir plates all the time? In the past I've let them grow out then used the stir plate "blend up" the new growth. Not sure if the better way however.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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D3_Myc
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Registered: 05/06/18
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I noticed much more and speedier growth once I put mine on the plate and let it spin on its lowest setting. But this is my very first LC so not sure what’s considered good, fast or bad growth. How does it look, It’s a super simple recipe of distilled water and .5g lme. Unmodded jar with plastic lid, intent is to just pour inoculate. My starter was 2 wedges of rw.



It was just a practice jar so I can get a feel for LC, I think for most part it’s doing good sans this little discolored spot that looks to be growing on a very small hair that must of hitched a ride I’m assuming pre-sterilization
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Mateja


Registered: 07/14/16
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Re: Some notes about LCs [Re: D3_Myc]
#27025715 - 11/06/20 08:39 PM (3 years, 3 months ago) |
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0.5% LME? How long ago did you inoculate?
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D3_Myc
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Re: Some notes about LCs [Re: Mateja]
#27025719 - 11/06/20 08:42 PM (3 years, 3 months ago) |
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My gut was 2g but other recommended that low. I need to look, was a few days ago. Less than a week
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Mateja


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Re: Some notes about LCs [Re: D3_Myc]
#27025730 - 11/06/20 08:49 PM (3 years, 3 months ago) |
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You've stated the amount of LME added two times by now and ai still have no clue about the nutrient density of your broth  Since it looks so clear I'm guessing it's 0.2%LME? As in: you added 2g LME into 1000ml water?
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D3_Myc
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Re: Some notes about LCs [Re: Mateja]
#27025735 - 11/06/20 08:51 PM (3 years, 3 months ago) |
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.5g to 500ml looking back I asked a question about nutes 6 days ago. Assuming I made the broth that night my culture probably 4-5 days old.
The brand of lme I use dissolves pretty clear. I boiled it and hit with stick blender just like I do with my agar
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Mateja


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Re: Some notes about LCs [Re: D3_Myc]
#27025803 - 11/06/20 09:36 PM (3 years, 3 months ago) |
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Do you have any tips for DIY stirrers? I have a bunch of magnets and computer fans that I'll be putting together tomorrow but I forgot to order some stirrers.
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A.k.a
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Re: Some notes about LCs [Re: Mateja]
#27025805 - 11/06/20 09:39 PM (3 years, 3 months ago) |
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You’re gonna love lc man.
It could be a combo of your super clear pictures and me not using a stir plate but those myc blobs look odd to me. Usually they’re way more fluffy looking.
The discolored spot you circled isn’t the agar wedge?
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LAGM2020     
Edited by A.k.a (11/06/20 09:42 PM)
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D3_Myc
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Re: Some notes about LCs [Re: A.k.a]
#27025818 - 11/06/20 10:07 PM (3 years, 3 months ago) |
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Once a day I spin the shit out it to tear them up. The pucks looked really cool growing out 3 dimensional.
That dark spot circled in read looked like a really fine hair with the darker stuff attached to it. Went to buy another stir plate. Didn’t realize I paid $60 for the damn thing. Be nice to have one with 3 stir spots.
I’m making a few more jars of various nute levels that won’t be on the stir plate. Can see how beat up they get making the jagged appearance
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PitcherCrab
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Re: Some notes about LCs [Re: D3_Myc]
#27025908 - 11/07/20 12:03 AM (3 years, 3 months ago) |
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What brand of LME is that Demonic? Both my agar and LCs have been ending up with sentiment for as long as I can remember and it drives me nuts. The mushrooms don't care, but I'd love to make everything a little clearer if possible. Or if you have other tricks to get them clearer I'd love to heard them. Your plates are beautiful.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Mateja


Registered: 07/14/16
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For agar I'd say pour thin whatever recipe you choose, to end up with as clear plates as possible. As for LC ime 0.05%LME makes for a crystal clear broth. (1g LME dissolved in 2L)
Looks like this, no sediment
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D3_Myc
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Quote:
PitcherCrab said: What brand of LME is that Demonic? Both my agar and LCs have been ending up with sentiment for as long as I can remember and it drives me nuts. The mushrooms don't care, but I'd love to make everything a little clearer if possible. Or if you have other tricks to get them clearer I'd love to heard them. Your plates are beautiful.
Brand, links and prep are all here https://www.shroomery.org/forums/showflat.php/Number/26628234#26628234
I think the pre-boil is the important part for getting it completely dissolved. If not stick blender I’m sure a wisk would work almost as well.
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Blue Helix
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Re: Some notes about LCs [Re: D3_Myc] 1
#27027363 - 11/07/20 06:14 PM (3 years, 3 months ago) |
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Edited by Blue Helix (11/07/20 06:28 PM)
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Blue Helix
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Quote:
PitcherCrab said: What brand of LME is that Demonic? Both my agar and LCs have been ending up with sentiment for as long as I can remember and it drives me nuts. The mushrooms don't care, but I'd love to make everything a little clearer if possible. Or if you have other tricks to get them clearer I'd love to heard them. Your plates are beautiful.
I've tried various things to make them clearer and the only one that worked is basically a pain in the ass. You can boil it for like five minutes and then strain it out with a coffee filter. The heat changes the sediments such that they can be filtered with an ordinary coffee filter, but I'm sure if you got the right filter from a chemical supply place, you could filter the stuff off without heating it up like that.
Still, I've grown to just accept the sediments. For one thing, as soon as the LC really takes off, it'll sequester the sediment anyway and become crystal clear, but yeah, they are kind of annoying until then. Given I can colonize an LC in 3 days once they are going fast and past the bacteria thing, those sediments are not annoying enough for me to change the late Agar's old formula that has served me so well, though:
10g DME or LME or ELME (aka dry malt extract, light malt extract, or extra light malt extract - they all worked fine) 10g dextrose (aka corn sugar) 500ml RO water
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Blue Helix
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Re: Some notes about LCs [Re: Blue Helix]
#27027397 - 11/07/20 06:42 PM (3 years, 3 months ago) |
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Here's the exact type I have mixed up now:
10g of $17 for 3 pounds Briess Dry Light Malt Extract 10g of $13 for 4 pounds Corn Sugar 500 ml RO or distilled water (tap probably works depending on where you live)
So those bags referenced above are enough for 68 liters of liquid culture or about 136 500-ml quart jars of the stuff, probably enough for a long time. I use a FoodSaver to vacuum seal the bulk of it with a 500cc oxygen absorber (link to the best deal I've seen) so it doesn't get like a brick or oxidize. The rest I just store in a zip lock in my refrigerator.
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BrownBear
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Quote:
PitcherCrab said: What brand of LME is that Demonic? Both my agar and LCs have been ending up with sentiment for as long as I can remember and it drives me nuts. The mushrooms don't care, but I'd love to make everything a little clearer if possible. Or if you have other tricks to get them clearer I'd love to heard them. Your plates are beautiful.
I use .5 grams of nutritional yeast in my LC's. I have sediment but after a few days at low speed on the stir plate the mycelium colonizes it all and it ends up crystal clear. Thats when you know its ready. When the mycelium has colonized all of the substrate.
Edited by BrownBear (11/07/20 07:42 PM)
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