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Alexjones
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Registered: 07/29/20
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Is this agar plate stalling out on me or just growing very slowly
#27025466 - 11/06/20 05:48 PM (3 years, 2 months ago) |
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This is a clone I have been working on and i feel like it is stalling out on me, the edge of the myc circle is puffed up I don’t see any yellow bacteria. Should I. Just go ahead and make a transfer?
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plutoniusspasticus
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Registered: 08/01/20
Posts: 61
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: Alexjones]
#27028784 - 11/08/20 03:02 PM (3 years, 2 months ago) |
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Quote:
Alexjones said: This is a clone I have been working on and i feel like it is stalling out on me, the edge of the myc circle is puffed up I don’t see any yellow bacteria. Should I. Just go ahead and make a transfer?

I think if it's stalling then it could be invisible contamination around the edge. I would transfer just before the edge to get a clean culture.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: plutoniusspasticus]
#27029205 - 11/08/20 08:30 PM (3 years, 2 months ago) |
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Nothing about that looks like the myc we hope to grow. What in the world is going on in there.
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GimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
Posts: 1,097
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: Alexjones]
#27029353 - 11/08/20 10:46 PM (3 years, 2 months ago) |
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How long has that chunk been chilling? Agar looks dry or hard, with weirdness like a dry film across it.
Some puffing up from the chunk of clone is to be expected the first day or so, then start to race outward.
If it's been longer then a week, I'd scrub that jar.
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Alexjones
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Registered: 07/29/20
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: GimpCollector]
#27032210 - 11/10/20 07:41 PM (3 years, 2 months ago) |
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I do not know what i did wrong this is my second transfer, it’s been more than two weeks could this be from temps getting too cold?
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Alexjones
Stranger


Registered: 07/29/20
Posts: 100
Last seen: 2 years, 1 month
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: Alexjones]
#27032252 - 11/10/20 08:13 PM (3 years, 2 months ago) |
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The temperature had been getting in the 40s during the night for a couple weeks I’m not sure if that would cause something like that, not sure how I cold it got in the closet I was storing them in
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Ryzuma

Registered: 02/19/19
Posts: 13
Last seen: 1 year, 2 months
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: Alexjones]
#27032282 - 11/10/20 08:32 PM (3 years, 2 months ago) |
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I would scrap it if its been two weeks and it looks like that
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sporecap
Shedding...

Registered: 07/30/18
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Loc: Europe
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: Ryzuma]
#27032723 - 11/11/20 03:28 AM (3 years, 2 months ago) |
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I don't know if it's the angle/lighting, but to me it looks like there is some obvious mold growing on the agar, encircling the myc in the center. That's why it's stalled.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Last seen: 1 year, 7 months
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Re: Is this agar plate stalling out on me or just growing very slowly [Re: sporecap] 1
#27033483 - 11/11/20 01:39 PM (3 years, 2 months ago) |
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It's not the lighting or angle. That plate is a pile of fuck knuckles. I don't know what it is but i know what it's not.
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