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Mr_enforcer1
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Flowhood contams
#27025358 - 11/06/20 04:34 PM (3 years, 2 months ago) |
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Moved to a new place and the room is a little bigger, not by much, but ALL my agar work is failing. Tried 20 different times. Tired cutting airflow and working right in front of the face still get the same contams. I was having 100% success before moving. Any suggestions what to try?
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mcnugget
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Not sure what would cause sudden contams if the hood functioned properly before. You check all the seams for leaks? Did it get knocked or bumped in the move? A friend had a flowhood in a storage unit for a few weeks and ended up having to replace the filter afterwards, everything he did contaminated.
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Mr_enforcer1
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Re: Flowhood contams [Re: mcnugget]
#27025384 - 11/06/20 04:54 PM (3 years, 2 months ago) |
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Well it was on a trailer so it was getting bumped around, why would it have to be replaced after a few weeks or months of storage?
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mcnugget
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Not really sure, it didn't get any water damage but the unit wasn't climate controlled and it was during a damp spring. He did find mice droppings in some boxes so maybe that had something to do with it.
Tried changing the pre-filter, cleaning it, etc but his contam rate was still unacceptable.
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3SIXTY5
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Re: Flowhood contams [Re: mcnugget]
#27025456 - 11/06/20 05:38 PM (3 years, 2 months ago) |
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If you haven't changed anything except for the place it must've gotten damaged, what's the filter face looking like?
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Mr_enforcer1
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Re: Flowhood contams [Re: 3SIXTY5]
#27025552 - 11/06/20 06:45 PM (3 years, 2 months ago) |
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Filter face looks fine, here’s a video of a lighter up to it
https://gofile.io/d/jr9TVp
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3SIXTY5
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Hmm, seems fine with the lighter test. What's ur agar prep like, do you use an all American PC with the round rocker weight?
I had an issue a couple weeks ago where two batches in a row completely contammed, and I did everything the same as I always do, and then I figured out that my weight for my PC was on the 5 psi side of the rocker, I guess my girl had knocked it off and put it back on not knowing that there were different settings, so I PC's two batches @ 5 psi.
Seems like an issue with sterilization if I had to guess, ur flow hood seems like it's blowing fine, idk man, spray it down with some h202 or something and give it another go.
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Mr_enforcer1
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Re: Flowhood contams [Re: 3SIXTY5]
#27026499 - 11/07/20 10:14 AM (3 years, 2 months ago) |
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There’s a small draft coming in from the door next to the flowhood, you think that’s enough to cause contams?
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dfwerydfhg
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Registered: 05/04/20
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Unless the draft is a significant fraction of 100 fpm I'd say not. Do you have side and top baffles, or just using a panel? Next step might be to try those.
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dfwerydfhg
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Oh, could also be your petris being non-sterile? Could be small holes in the bags from shit handling somewhere, or if they're long expired.
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3SIXTY5
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Could be the draft, what's ur agar prep like tho? When r they contaminating? You're just pouring dishes and wrapping them for later use and they're getting contammed or what?
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Mr_enforcer1
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Re: Flowhood contams [Re: 3SIXTY5]
#27029102 - 11/08/20 06:57 PM (3 years, 2 months ago) |
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I already checked the plates, they’re not the problem. Forgot to mention I’m using MS syringe to agar. Never had a problem with the same syringes in the past.
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dfwerydfhg
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Registered: 05/04/20
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Wait, so you're pouring your plates ok without them contaminating first? Then they only go bad after you add MS to them? Sounds like a big clue right there... is the contam always the same, or varied?
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KKVguitarist
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Look at your filter, if it is a wood framed filter they can shift and cut through the filter.material with the metal baffles. Check for any rips or punctures in the material
If you need a new hood I build and sell them and almost have the new website up to make the operation a bit more.legit
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trippleblack
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Quote:
KKVguitarist said: Look at your filter, if it is a wood framed filter they can shift and cut through the filter.material with the metal baffles. Check for any rips or punctures in the material
If you need a new hood I build and sell them and almost have the new website up to make the operation a bit more.legit
I strongly suggest everyone to avoid dealing with this guy..
very bad news in more ways than one.
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Smartattack
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Registered: 12/21/18
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Loc: A thought
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Do tell.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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trippleblack
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i dropped a line or two about this guy in the marketplace. i don't want a back n forth with him; and airing him out in a open forum will get into murky territory. you can pm me if it's that serious. i am 100% certain others have heard of the infamous KKVguitarist around these parts too.
i'm dying to drop the tea. but i just cant.. if anyone search his name i did my part by properly putting smut on it.
Edited by trippleblack (11/09/20 08:09 PM)
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Mr_enforcer1
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I guess it was the syringes. I started some agar transfers and all success. Thanks for the help guys
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