Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Flowhood contams
    #27025358 - 11/06/20 04:34 PM (3 years, 2 months ago)

Moved to a new place and the room is a little bigger, not by much, but ALL my agar work is failing. Tried 20 different times. Tired cutting airflow and working right in front of the face still get the same contams. I was having 100% success before moving. Any suggestions what to try?


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Offlinemcnugget
Stranger


Registered: 06/22/13
Posts: 260
Last seen: 1 month, 17 days
Re: Flowhood contams [Re: Mr_enforcer1]
    #27025382 - 11/06/20 04:52 PM (3 years, 2 months ago)

Not sure what would cause sudden contams if the hood functioned properly before.  You check all the seams for leaks?  Did it get knocked or bumped in the move?  A friend had a flowhood in a storage unit for a few weeks and ended up having to replace the filter afterwards, everything he did contaminated.


--------------------


Extras: Filter Print Post Top
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Re: Flowhood contams [Re: mcnugget]
    #27025384 - 11/06/20 04:54 PM (3 years, 2 months ago)

Well it was on a trailer so it was getting bumped around, why would it have to be replaced after a few weeks or months of storage?


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Offlinemcnugget
Stranger


Registered: 06/22/13
Posts: 260
Last seen: 1 month, 17 days
Re: Flowhood contams [Re: Mr_enforcer1]
    #27025409 - 11/06/20 05:12 PM (3 years, 2 months ago)

Not really sure, it didn't get any water damage but the unit wasn't climate controlled and it was during a damp spring.  He did find mice droppings in some boxes so maybe that had something to do with it. 

Tried changing the pre-filter, cleaning it, etc but his contam rate was still unacceptable.


--------------------


Extras: Filter Print Post Top
Offline3SIXTY5
Stranger
 User Gallery
Registered: 03/27/20
Posts: 62
Last seen: 2 years, 3 months
Re: Flowhood contams [Re: mcnugget]
    #27025456 - 11/06/20 05:38 PM (3 years, 2 months ago)

If you haven't changed anything except for the place it must've gotten damaged, what's the filter face looking like?


Extras: Filter Print Post Top
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Re: Flowhood contams [Re: 3SIXTY5]
    #27025552 - 11/06/20 06:45 PM (3 years, 2 months ago)

Filter face looks fine, here’s a video of a lighter up to it

https://gofile.io/d/jr9TVp


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Offline3SIXTY5
Stranger
 User Gallery
Registered: 03/27/20
Posts: 62
Last seen: 2 years, 3 months
Re: Flowhood contams [Re: Mr_enforcer1]
    #27025675 - 11/06/20 07:59 PM (3 years, 2 months ago)

Hmm, seems fine with the lighter test. What's ur agar prep like, do you use an all American PC with the round rocker weight?

I had an issue a couple weeks ago where two batches in a row completely contammed, and I did everything the same as I always do, and then I figured out that my weight for my PC was on the 5 psi side of the rocker, I guess my girl had knocked it off and put it back on not knowing that there were different settings, so I PC's two batches @ 5 psi.

Seems like an issue with sterilization if I had to guess, ur flow hood seems like it's blowing fine, idk man, spray it down with some h202 or something and give it another go.


Extras: Filter Print Post Top
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Re: Flowhood contams [Re: 3SIXTY5]
    #27026499 - 11/07/20 10:14 AM (3 years, 2 months ago)

There’s a small draft coming in from the door next to the flowhood, you think that’s enough to cause contams?


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Invisibledfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
Re: Flowhood contams [Re: Mr_enforcer1]
    #27026615 - 11/07/20 11:18 AM (3 years, 2 months ago)

Unless the draft is a significant fraction of 100 fpm I'd say not. Do you have side and top baffles, or just using a panel? Next step might be to try those.


Extras: Filter Print Post Top
Invisibledfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
Re: Flowhood contams [Re: dfwerydfhg]
    #27026618 - 11/07/20 11:20 AM (3 years, 2 months ago)

Oh, could also be your petris being non-sterile? Could be small holes in the bags from shit handling somewhere, or if they're long expired.


Extras: Filter Print Post Top
Offline3SIXTY5
Stranger
 User Gallery
Registered: 03/27/20
Posts: 62
Last seen: 2 years, 3 months
Re: Flowhood contams [Re: Mr_enforcer1]
    #27027156 - 11/07/20 04:29 PM (3 years, 2 months ago)

Could be the draft, what's ur agar prep like tho? When r they contaminating? You're just pouring dishes and wrapping them for later use and they're getting contammed or what?


Extras: Filter Print Post Top
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Re: Flowhood contams [Re: 3SIXTY5]
    #27029102 - 11/08/20 06:57 PM (3 years, 2 months ago)

I already checked the plates, they’re not the problem. Forgot to mention I’m using MS syringe to agar. Never had a problem with the same syringes in the past.


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Invisibledfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
Re: Flowhood contams [Re: Mr_enforcer1]
    #27030005 - 11/09/20 11:10 AM (3 years, 2 months ago)

Wait, so you're pouring your plates ok without them contaminating first? Then they only go bad after you add MS to them? Sounds like a big clue right there... is the contam always the same, or varied?


Extras: Filter Print Post Top
OfflineKKVguitarist
Mycogeek
Male User Gallery


Registered: 12/24/14
Posts: 389
Loc: Jupiter
Last seen: 1 year, 5 months
Re: Flowhood contams [Re: dfwerydfhg]
    #27030700 - 11/09/20 07:46 PM (3 years, 2 months ago)

Look at your filter, if it is a wood framed filter they can shift and cut through the filter.material with the metal baffles. Check for any rips or punctures in the material

If you need a new hood I build and sell them and almost have the new website up to make the operation a bit more.legit


Extras: Filter Print Post Top
Offlinetrippleblack
Stranger
 User Gallery
Registered: 12/01/19
Posts: 355
Last seen: 1 day, 16 minutes
Re: Flowhood contams [Re: KKVguitarist]
    #27030713 - 11/09/20 07:55 PM (3 years, 2 months ago)

Quote:

KKVguitarist said:
Look at your filter, if it is a wood framed filter they can shift and cut through the filter.material with the metal baffles. Check for any rips or punctures in the material

If you need a new hood I build and sell them and almost have the new website up to make the operation a bit more.legit




I strongly suggest everyone to avoid dealing with this guy..

very bad news in more ways than one.


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,775
Loc: A thought
Trusted Cultivator
Re: Flowhood contams [Re: trippleblack]
    #27030718 - 11/09/20 08:00 PM (3 years, 2 months ago)

Do tell.

:wonka:


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Offlinetrippleblack
Stranger
 User Gallery
Registered: 12/01/19
Posts: 355
Last seen: 1 day, 16 minutes
Re: Flowhood contams [Re: Smartattack]
    #27030726 - 11/09/20 08:07 PM (3 years, 2 months ago)

i dropped a line or two about this guy in the marketplace.  i don't want a back n forth with him; and airing him out in a open forum will get into murky territory.  you can pm me if it's that serious.  i am 100% certain others have heard of the infamous KKVguitarist around these parts too.

i'm dying to drop the tea. but i just cant.. if anyone search his name i did my part by properly putting smut on it.


Edited by trippleblack (11/09/20 08:09 PM)


Extras: Filter Print Post Top
OfflineMr_enforcer1
Finder-less
Male User Gallery

Registered: 05/28/18
Posts: 383
Loc: United States
Last seen: 1 year, 3 months
Re: Flowhood contams [Re: trippleblack]
    #27037225 - 11/13/20 02:52 PM (3 years, 2 months ago)

I guess it was the syringes. I started some agar transfers and all success. Thanks for the help guys :irishtoast:


--------------------
HOW TO UPLOAD A PICTURE



Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Flowhood without hepa filter? TheShroomHermit 1,211 3 04/05/03 12:15 AM
by SixTango
* Re: Substituting dry verm. with a filter disk... BaldCuban 809 3 05/28/00 12:50 AM
by vts1134
* update from40jars lost(contams growing thru filter cbull 4,563 16 09/20/08 05:21 PM
by RogerRabbit
* which blower must i use for this hepa filter? yoko 1,511 4 10/22/03 08:11 AM
by dastats
* Growth log of questionable G2G spawn (1st Grow ever) *DELETED* zushiZoots 444 11 12/14/20 07:58 AM
by zushiZoots
* Cheap permeant jar lid filter.
( 1 2 3 all )
SixTango 12,298 52 01/29/03 09:31 AM
by shroomologist
* What the hell is a flowhoods use? myco_holic 2,523 12 10/08/02 09:07 PM
by SixTango
* Using Filters Of PF Jars Anonymous 536 1 05/07/03 03:29 AM
by woodrow

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
331 topic views. 28 members, 217 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 14 queries.