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turtlesonturtles
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Monoculture identification
#27023095 - 11/05/20 11:24 AM (3 years, 2 months ago) |
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Hey. You don’t know me but I know you <3 (first post)
Are these mono?
Thanks!
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The Mycologist
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Doesnt look like it. How many transfers did you make?
Why go for mono anyways? It could be a non fruiting strain.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Kmacmo
The aborted pin


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Agreed doesn't look like it's there yet. How many transfers? Its a cool side project to do, feels like you have a lottery ticket and just waiting for that one badass monoculture to come around.
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GimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
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Interesting. I've always been under the impression that mono culture is the way to go for consistent tubs and flushes.
Taking flesh from inside a boomer, would that not be a mono, at least you know the mycelium fruited. I dunno I clone from a chunk inside the fruit.
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ModularMind
M.P.F.



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Quote:
GimpCollector said: Interesting. I've always been under the impression that mono culture is the way to go for consistent tubs and flushes.
Taking flesh from inside a boomer, would that not be a mono, at least you know the mycelium fruited.
If you sector on agar it may not be a fruiting culture. If you take a clone from a fruit it has proven to be fruitful.
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Kmacmo
The aborted pin


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Re: Monoculture identification [Re: ModularMind] 1
#27023334 - 11/05/20 01:55 PM (3 years, 2 months ago) |
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A monoculture is a single set of genetics, with no sectoring. It does have to be fruited for testing but you may just find a really good one if you have the patience. Taking tissue from a fruit would give you a clone and will speed things up in the monoculture process instead of going from MS to mono.
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GimpCollector
To Drunk To Taste The Chicken

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Re: Monoculture identification [Re: Kmacmo]
#27023455 - 11/05/20 03:18 PM (3 years, 2 months ago) |
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Thank you for your reply. Mono culture vs non on dish, what does that look like, I guess myself and OP (Thanks OP) don't know what mono vs non sectors look like... Would love a side by side comparison, if there is one...
OP here's a great read (At least for me)
https://www.shroomery.org/forums/showflat.php/Number/20287172/fpart/all/vc/1
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turtlesonturtles
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Re: Monoculture identification [Re: Kmacmo]
#27025982 - 11/07/20 01:42 AM (3 years, 2 months ago) |
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Ahh chucks - thought I had it. Probably 7/8 transfers.
Thanks for the replies people!
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turtlesonturtles
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OP again. Probably 3/4 transfers later. Am I there yet? Thanks!

PS: I always do 20 transfers at a time, and these are the only ones that look even close. I must be at 10+ rounds of transfers at this point - it’s getting a bit frustrating. Is it possible to fuck this up somehow? Transfering too large sectors, perhaps?
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turtlesonturtles
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Bumping with new pic - help very much appreciated!

PS: My cultures seem to be getting shittier -I’m pretty sure they take longer to colonize a plate of agar now than they did in the beginning. Or...?
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Sold Out Online
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Whatever you’re going to do with them, you should probably do.
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The Mycologist
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Quote:
Sold Out Online said: Whatever you’re going to do with them, you should probably do.

I think you have a hundred or so transfers left lol. Monoculture isnt really worth it.
If you really want a mono, start with mushroom tissue instead of spores.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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turtlesonturtles
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For real? I saw a clip from «Let’s Grow Mushrooms» on YT where he said it would take like 7-8 transfers... oh well. I’ll do tissue samples then.
Also how is it not worth it? My last batch of 45qt tubs using 1,5-2kg (roughly 4-5 qts) spawn bags and 1:2-2:3 spawn:coir ratio averaged about 70 grams dried. I thought with the right genetics you could get to an average of 200g easily?
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Kmacmo
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I just read that you do 20 transfers each time, you don't take 20 transfers from a single plate do you? Yeah a good monoculture is worth it, the fastest most potent and most yeild of shrooms is any growers dream just nobody can be bothered doing all that work just for some improvement when regular MS mushrooms are usually good enough for most people
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Professor X
School for the Gifted



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Re: Monoculture identification [Re: Kmacmo]
#27115934 - 12/30/20 10:25 AM (3 years, 29 days ago) |
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That is not a mono. My ks mono took over a year and I have tossed dozens of others in the process. Mono requires patience and they can also produce weird results or nothing at all. Have had both happen at different times.
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turtlesonturtles
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Re: Monoculture identification [Re: Kmacmo]
#27117484 - 12/31/20 03:02 AM (3 years, 28 days ago) |
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Quote:
Kmacmo said: I just read that you do 20 transfers each time, you don't take 20 transfers from a single plate do you?
No - I make a full sleeve (20) of plates every time I do transfers, and I transfer from the best looking plates from the last batch (usually around 10 of them, so 2 transfers per plate).
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jcm4620
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i spent over a yr getting a mono only for it to not even fruit anything at all. i no longer even bother with such things. id rather get my shit on grain as soon as possible and start fruiting and find clones tben tfansfer down and fruit out every one and save the best
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