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gabbk
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Why the bottom of these bags aren't colonizing well?
#27021192 - 11/04/20 10:43 AM (3 years, 3 months ago) |
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Hey guys! I made a couple bags of oyster bags with pasteurized wood pellets following lipa's tek, and I tied the end of every one of these bags with a regular seal, leaving a little/medium gap of air, and the only sectors that seems to be colonizing are near the tie. Am I doing something wrong? Like this one. This bag has already 11 days and looks soooo delayed They are sitting in my living room, at around 65-68F.

And these other bags I made yesterday too.

I am 100% sure the spawns were healthy! (Colonized grains in less than 2 weeks) What should I do?
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27021265 - 11/04/20 11:28 AM (3 years, 3 months ago) |
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A friend of mine suggested opening more the GE hole, so I did it!
 Let's see how it goes
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Harry Manbach
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27021268 - 11/04/20 11:29 AM (3 years, 3 months ago) |
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Seems cold for incubation. Those seem like better grow room temps! I'd try getting the temps up into the 70's more.
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Harry Manbach
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27021271 - 11/04/20 11:31 AM (3 years, 3 months ago) |
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Quote:
gabbk said: A friend of mine suggested opening more the GE hole, so I did it!
 Let's see how it goes
Definitely wouldn't have done that...why would you open the bag to expose raw substrate to open air only to put micropore right back over it!?
How do you inoculate your bags? Flow hood..open air?
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: Harry Manbach]
#27021279 - 11/04/20 11:35 AM (3 years, 3 months ago) |
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Well. I could try upping the temps also. I didn't open the bag. I just put a micropore tape, made a little hole and put another micropore on it. I don't think it will get hurt for doing that. It was less than 5 seconds lol. By the way, I inoculate my pellet bags with the spawn in open air
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Harry Manbach
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27021294 - 11/04/20 11:43 AM (3 years, 3 months ago) |
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5 seconds .5 seconds doesn't matter..it's the least of your issues right now,but you did expose the substrate.
However, I hadn't even noticed you weren't using filter bags! So yeah that's definitely a problem starting them with no gas exchange. I don't know if you're supplementing or not, but if you are,you want to minimize the exposure to open air!
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Harry Manbach
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Re: Why the bottom of these bags aren't colonizing well? [Re: Harry Manbach]
#27021298 - 11/04/20 11:45 AM (3 years, 3 months ago) |
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If you're not supplementing..or even if you are try adding more spawn to Filter bags next time, you'll have much better results!
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deadmandave
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Re: Why the bottom of these bags aren't colonizing well? [Re: Harry Manbach]
#27021346 - 11/04/20 12:12 PM (3 years, 3 months ago) |
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Quote:
Harry Manbach said:
Quote:
gabbk said: A friend of mine suggested opening more the GE hole, so I did it!
 Let's see how it goes
Definitely wouldn't have done that...why would you open the bag to expose raw substrate to open air only to put micropore right back over it!?
How do you inoculate your bags? Flow hood..open air?
These are "pasteurized" substrate so its fine to expose them to the elements. I agree that they were lacking GE and would have advised stabbing some holes in the bottom and sides.
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: deadmandave]
#27021602 - 11/04/20 02:38 PM (3 years, 3 months ago) |
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Yeah! I didn't even worry so much because it has not supplements. It's just pasteurized pellets
And.. damn! Should I make more holes, sides and bottom too? I though with that with just one at the top (as I shown in the last pic) was enough
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deadmandave
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27022113 - 11/04/20 06:56 PM (3 years, 3 months ago) |
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I dont think it would hurt to poke a few holes at the bottom.
normally with filter patch bags theres just the one GE port
could be something else like too much water in the substrate. If it was too low GE they might have a hard time recovering after 11 days.
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teknix
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Re: Why the bottom of these bags aren't colonizing well? [Re: deadmandave]
#27022340 - 11/04/20 09:00 PM (3 years, 3 months ago) |
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Are those Hardwood fuel pellets?
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: teknix]
#27022375 - 11/04/20 09:28 PM (3 years, 3 months ago) |
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Ohhhh, I see... It makes sense deadmandave. Well. Im gonna do one more hole at the bottom of each bag. And if it doesn't work well I definitely will adjust a little bit the water content the next time. These bags are all 55% water - 45% wood* pellets. I don't know why I thought that being close to none GE was beneficial for oysters colonization. I'm sure I misread.
*Btw, it's softwood pellets. My vendor suggested me to use certain brand of softwood fuel pellets. He's a big scale grower and it's the same wood he uses also for growing reishi and lion's mane. I had success myself with two grows before with it. It seems that the mushroom doesn't have any trouble to eat it; the material feels very soft to the touch when pasteurized. Anyways, in these two previous grows I encountered the same problem, both delayed minimum 5 weeks to fully colonize and fruit. And the flushes were between 2-3 weeks apart. The thing is that both of them had almost zero GE when colonizing and I didn't know it could be an issue at the time. Maybe the lack of GE favored any other microorganism to grow and that's why it took so long, specially in between flushes too.
So.. we are going to find out in this third run if the issue was the GE or the water content!
Edited by gabbk (11/04/20 09:37 PM)
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teknix
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27022432 - 11/04/20 10:38 PM (3 years, 3 months ago) |
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Well, it looks like you have direct evidence that your mycelium hates it.
Try hardwood next time and then compare the results.
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: teknix]
#27022655 - 11/05/20 06:00 AM (3 years, 3 months ago) |
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I will! Thanks for all the feedback guys, I really appreciate it!
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Cyonic
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27269628 - 03/26/21 12:47 AM (2 years, 10 months ago) |
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Supplemented doug fir (softwood) will work fine.
It may be that your vendor who uses that type of softwood for commercial growing is adding bran or something and then sterilizing everything before spawning and that is why they are successful. I would contact them and ask if they are supplementing.
If that is the case that would explain the slow colonization.
Reishi grows fine on softwood. I believe pine is it's natural habitat so if they are growing Reishi on softwood it would be fine.
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: Cyonic]
#27269786 - 03/26/21 06:00 AM (2 years, 10 months ago) |
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Cyronic Right on. Thanks lot for the input, I wouldn't have imagined it, it makes a lot of sense sense that it'd work if it's supplemented and sterilizedππ Interestingly enough I've been playing around non sterile methods and found out that the best thing for me is just add cold water to the pellets. I made a comparison with oysters on pellets pasteurized lipa way (which I've been doing all this time) vs hydrated with just cold water vs cold water also but with some vinegar added (10%), and noticed the cold water only was the quickest to colonize. The ones pasteurized with boiling water all (lipa's tek) always got really slow to colonize and got foul smell. And the ones with vinegar added they all went south with trichoderma. I wonder why the cold water only was the best one. I thought somewhat processing the pellets, at least with some heat would be useful
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Cyonic
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Re: Why the bottom of these bags aren't colonizing well? [Re: gabbk]
#27274163 - 03/29/21 09:06 AM (2 years, 10 months ago) |
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That is interesting about the cold water. That is not what I would expect. I wonder what the cause of that could be?
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gabbk
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Re: Why the bottom of these bags aren't colonizing well? [Re: Cyonic]
#27274181 - 03/29/21 09:28 AM (2 years, 10 months ago) |
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Yes that's exactly why it surprised me also, I didn't expect it to function initially, at all to be honest. I just did it for the sake of experimenting and giggles. I thought about it and maybe it's because the pellets already come pasteurized within the industrialization process, so just adding cold water would be enough, while adding hot water would make sort of a mess with the microclimate because of the hot steam, specially in some areas such as the bottom of the pot/container, favoring anaerobic microorganisms to grow. I just came with that idea when I realised that when every time I prepared the pellets, the smell was always kind of dirty, kinda like bacterial smell if you can recognize it (acidic, or fruity like). And now I remember the odor with cold water, it was definitely much more clean, just the smell of wood. I couldn't find a concrete answer really, so that's my best guess.
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