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frikiriki
Shroom Nerd

Registered: 11/04/20
Posts: 17
Last seen: 9 months, 18 days
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mycelium growth behavior change on different agar medium
#27021946 - 11/04/20 05:30 PM (3 years, 2 months ago) |
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Hello!
I am currently working on sub-selections to multiply healthy Blue Meanie dishes.
They were first grown on malt dextrose agar, and were sub-selected and transferred once using the same agar recipe.
I Tried An Experiment
I transferred two-thirds to malt again, and one-third to a new agar recipe involving rye grain soak water.
The sub-selection to malt agar dish transfers are going great, the mycelium picked up fast.
The rye agar dish sub-selections seemed to be shocked, very fuzzy, and stunted.
The introduction of the rye grain soak water to the agar growth period was done with two questions in mind...
1. Could using the rye agar possibly help the mycelium with the shock of being transferred to the rye grain bags? (They would already "know" the environment.)
2. If I sub select several times over, will the mycelium perform better when given a different/new nutrient source?
I have heard of success with rye grain soak water in agar, but cannot find anything on sub-selections to new agar mediums.
I am currently growing spores and clones on a mix of rye and malt agar dishes to find out more.
Have any shroomery users also encountered this? Or know some know-how on this subject?
Thanks for reading and have a good day!
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MushingGoodTime
Stranger Things
Registered: 05/05/18
Posts: 53
Loc: Australia
Last seen: 1 year, 22 days
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Re: mycelium growth behavior change on different agar medium [Re: frikiriki]
#27027151 - 11/07/20 04:27 PM (3 years, 2 months ago) |
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Quite interested in this so following. I'm certainly no expert but I think in principle it makes sense. My understanding (and others will know more) that the myc can adapt it's protein excretions to break down material that it finds in it's travel i.e. adapt. So I guess it makes sense to start that process on Agar.
Keep us posted!
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Gnidrolog
Majestic Poobah


Registered: 11/10/20
Posts: 20
Loc: omotion
Last seen: 3 years, 1 month
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Re: mycelium growth behavior change on different agar medium [Re: frikiriki]
#27033947 - 11/11/20 06:10 PM (3 years, 2 months ago) |
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Interested in how this goes. Have 4 qts. of oat water (saved from hydration/boil) that I plan on using with agar. Wondering if I should make plates with it using MEA or plain agar. The hope is to select myc that seems to favor oats. I'll follow up with a report if anyone is interested.
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Vinci
I'm You



Registered: 08/26/19
Posts: 629
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Re: mycelium growth behavior change on different agar medium [Re: Gnidrolog]
#27053893 - 11/23/20 12:57 PM (3 years, 2 months ago) |
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I like to switch nutrients every transfer or two. I just make one batch of MEA and one batch of DFA and color them differently. The nutrient variety seems to keep the myc excited. I really like the DFA because theres mad variety within it too. For this same reason I make my own grainola for spawn. 4 parts oats, 3 parts wheat, 2 parts milo, 1 part barley. They each retain water differently, it makes it harder to over/under hydrate them. The barley makes it easy to break up and the milo provides mad inoculation points. Definitely colonizes faster than straight wheat, although I may try straight oats. I haven't tried but I bet my grainola soak water would have the same kick as DFA.
-------------------- Vinci loves you
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frikiriki
Shroom Nerd

Registered: 11/04/20
Posts: 17
Last seen: 9 months, 18 days
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malt dextrose agar > rye water agar [Re: Vinci]
#27087888 - 12/13/20 08:18 PM (3 years, 1 month ago) |
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Thanks for the interest!
I have concluded my experiment. I figured that the malt dextrose agar works just fine, and seems to be what the blue meanie mycelium prefers in most cases.
The rye water agar did seem to positively affect some golden teacher and ape dishes. It looked as though they were cautious at first, and then picked up pace pretty fast.
But the unpredictability of how the rye agar might affect different sub varieties has me sticking to malt dextrose.
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