Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds High THC Strains   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Glass petris
    #27019072 - 11/03/20 10:07 AM (3 years, 2 months ago)

Ok, so after receiving another(first ones arrived shattered, no packaging whatsoever) shipment of 100mm petris, I sanitized them, wrapped them individually in foil, made 2 packs of 4 petris.

Wrapped those 2 packs with two layers of foil.
Put everything in the oven, climbed up to 340F, waited for about 5mins, then started the countdown, stayed above 340F for aprox. 70min.

Can I just handle that packs up until the point I actually unwrap the whole deal inside the SAB?
Or is the sterility compromised as soon as I take them out of the oven?
Or will they stay sterile and can actually use them whenever I cook up some agar...? Maybe unwrap the first layer of the pack right before placing them in a SAB, leave the most of the gained contaminants outside? due to lack of experience with agar work..I mean I know that probably it's best to pour and tape the petris as soon as possible, due to time of exposure factor alone, but still, did I screw petris by taking those two packs out the oven and put them in a closet? ..which is clean tho..:laugh:
Anyhow, let me know your thoughts.

Also, is it possible that some petris actually cracked inside the oven? It seemed like I heard a few pings  two times..I was only overshooting for a couple of seconds, up to 360F..No sudden cooling or heating up..I didn't want to unwrap to take a look not to compromise the rest of them..

Thanks.
Cheers:mushroom2::rocket:


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: Sandstone]
    #27019087 - 11/03/20 10:18 AM (3 years, 2 months ago)

Ok, just found out that I probably maintained that temp one whole hour short..should have stayed above 340F for like 120min?
How high can I overshoot temperature wise and still keep my petris whole?
Kitchen ovens have crazy inaccurate thermostats, I just want to know how tedious do I have to be, standing there with a K-Type probe, trying to keep swings +- 50F..
Thanks


Extras: Filter Print Post Top
Offlinepolaritymind
relaxed attention
I'm a teapot

Registered: 10/10/16
Posts: 994
Loc: Germany Flag
Last seen: 3 months, 8 days
Re: Glass petris [Re: Sandstone]
    #27019120 - 11/03/20 10:42 AM (3 years, 2 months ago)

sub


--------------------
"to affirm life is to also affirm death"
-Albert hofmann


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: polaritymind]
    #27019129 - 11/03/20 10:49 AM (3 years, 2 months ago)

?


Extras: Filter Print Post Top
OfflineMushRock
Stranger
Male User Gallery


Registered: 10/20/20
Posts: 40
Loc: The netherlands, Utrecht
Last seen: 3 years, 1 month
Re: Glass petris [Re: Sandstone]
    #27019139 - 11/03/20 11:02 AM (3 years, 2 months ago)

Hi Sandstone,

I'm pretty noob myself and new to this forum. But I do have started myself with Glas petri dished, 100mm diagonal. My glass is OK to 600Degrees Celsius! so It should not crack from heat itself.

But sudden changes in temperature could be fatal. Glass is fragile so handle with care.

I don't use SAB or Oven. Just PC and work in open air (I live alone so not lot of air movement) but do notice some contams growing. But my contamination is starting on top of Agar, not inside.

From what I've red 20 minutes at high temp will do. 60 minutes or even more seems like overkill and presumably will dry out the agar. Not sure if oven time is equal, But I suspect even less time is required due to higher temps.

Wish you good luck at growing!


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Glass petris [Re: MushRock]
    #27019161 - 11/03/20 11:15 AM (3 years, 2 months ago)

If you don't end up hating glass dishes more power to you. I used oven sterilization. But then started buying plastic dishes because they're way superior.


Extras: Filter Print Post Top
OfflineMushRock
Stranger
Male User Gallery


Registered: 10/20/20
Posts: 40
Loc: The netherlands, Utrecht
Last seen: 3 years, 1 month
Re: Glass petris [Re: bodhisatta]
    #27019206 - 11/03/20 11:39 AM (3 years, 2 months ago)

Not sure To hate or to love, yet. Guess I'll find out.
Had grown some b+ shrooms 5 to 6 years ago, and because of covid-19 measurements I've now got lots of spare time to spend. So I got the idea of growing shrooms again.

Got some spores on agar:  costa rica and and 5 non active strains (clone from supermarket store).
Will post in a new tread when I've made more progress and photo's.


Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 22 hours
Re: Glass petris [Re: Sandstone]
    #27019212 - 11/03/20 11:43 AM (3 years, 2 months ago)

Don't listen to glass haters wearing turbans!  :laugh2:

Try: Sterilizing glass petris -Kizzle

" 1 hour at 450F should be sufficient but I usually leave it in for 2 because I don't have a lot of faith in Wikipedia:tongue: "


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: MushRock]
    #27019222 - 11/03/20 11:47 AM (3 years, 2 months ago)

That's awesome, go glass, save the planet haha :laugh: no but really man, the amount of shit one household is able to produce...breathtaking really, absolutely not trying to preach but that's the least I can do, besides I have enough time and will power lately to go trough the hassles of sterilization and clunkiness.

I have plastic ones en route, just in case none of my glass ones survives, for whatever reason..:laugh: the goal is still to produce mushrooms, I just consider plastic a last resort. Although on the other hand, the amount of tinfoil, oven time, sanitizing, and all that jazz on the other hand..I don't know man  :whiteflag:  :laugh:

I don't have the agar in the dishes as I'm oven sterilizing them. My plan was to keep them sterile and wrapped, till I make about 500cc of agar to pour, unpack them inside the SAB, pour the agar, tape with parafilm or just stretch foil, cool them down and store for use.

Thanks, likewise!
Cheers:mushroom2::rocket:


Edited by Sandstone (11/03/20 11:48 AM)


Extras: Filter Print Post Top
OfflineMushRock
Stranger
Male User Gallery


Registered: 10/20/20
Posts: 40
Loc: The netherlands, Utrecht
Last seen: 3 years, 1 month
Re: Glass petris [Re: Sandstone]
    #27019236 - 11/03/20 11:59 AM (3 years, 2 months ago)

I don't want to claim this tread but Yes.
"the amount of shit one household is able to produce"
This is what i'm concerned about. But to use PC on agar filled petri dishes is not great. lots of condensation.
I am trying out to use pour technique, but my 1st attempts was while using little too hot agar mixture. So next plates also got condense.


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: Sandstone]
    #27019241 - 11/03/20 12:00 PM (3 years, 2 months ago)

Wait, 450F for two hours? Ok, I just checked what I actually ordered and it appears that it's borosilicate glass, which should make it withstand quite a heat right? I got the even heating and cooling sorted so if they cracked it was not because of sudden temp change. Idk, will see once I unwrap, I'll probably manage to pour tomorrow :thumbup:
Thanks for that thread, I've just been reading it,  and in fact realized that I probably missed quite a few things..:laugh:
Cheers:mushroom2::rocket:


Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 22 hours
Re: Glass petris [Re: Sandstone]
    #27019243 - 11/03/20 12:01 PM (3 years, 2 months ago)

I love my Corning Borosilicate 100x15mm plates, they're beautiful and
I can pour as often as I please! This from my agar journal:

11/1
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, no color
plates wrapped in foil 450 F, 2 hrs
Try media bottle in pot of 130 F water
21 plates, 6 stack ok, heavy
Enter in Agar Agar Sheet
Instant Pot warm=160F, Crockpot = 130
Into Crockpot 12:50 130F
Poured 25, 2:10
Wrap bottom plate of stacks, wrapped 2:50
(wrapped bottom plate because I'm still letting the bottom
plate drop a bit) wrapped stacks of five for later!

I will also pour plastic plates to store in the fridge.


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: MushRock]
    #27019254 - 11/03/20 12:04 PM (3 years, 2 months ago)

Quote:

MushRock said:
I don't want to claim this tread but Yes.
"the amount of shit one household is able to produce"
This is what i'm concerned about. But to use PC on agar filled petri dishes is not great. lots of condensation.
I am trying out to use pour technique, but my 1st attempts was while using little too hot agar mixture. So next plates also got condense.




I was thinking of putting the SAB on top of a heating mat, set to full power heating. Petri dishes would get slightly heated (to aprox. 100F, closing the temperature gap between the pour ready agar and empty petri dishes. You could then, if needed, cool them down slower than air cooling, which should in turn minimize condensation?


Edited by Sandstone (11/03/20 12:06 PM)


Extras: Filter Print Post Top
OfflineMushRock
Stranger
Male User Gallery


Registered: 10/20/20
Posts: 40
Loc: The netherlands, Utrecht
Last seen: 3 years, 1 month
Re: Glass petris [Re: Sandstone]
    #27019267 - 11/03/20 12:09 PM (3 years, 2 months ago)

I do have a heating mat and will try it! The glass is still allowing some air exchange as I happen to see no condensation around the glass edges on the lid. Probably will work and solve my condensation issue. Just no SAB yet:confused:


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: Inthepit]
    #27019271 - 11/03/20 12:11 PM (3 years, 2 months ago)

Quote:

Inthepit said:
I love my Corning Borosilicate 100x15mm plates, they're beautiful and
I can pour as often as I please! This from my agar journal:

11/1
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, no color
plates wrapped in foil 450 F, 2 hrs
Try media bottle in pot of 130 F water
21 plates, 6 stack ok, heavy
Enter in Agar Agar Sheet
Instant Pot warm=160F, Crockpot = 130
Into Crockpot 12:50 130F
Poured 25, 2:10
Wrap bottom plate of stacks, wrapped 2:50
(wrapped bottom plate because I'm still letting the bottom
plate drop a bit) wrapped stacks of five for later!

I will also pour plastic plates to store in the fridge.




Yeah, always enjoyed glassy lab stuff, dont know why, stuck with me since high school chem classes XD
Thanks for that recipe, looks awesome, only thing is I only interpret about 25% of it :laugh: would you mind to extract? :laugh:
Thanks


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: MushRock]
    #27019274 - 11/03/20 12:12 PM (3 years, 2 months ago)

Quote:

MushRock said:
I do have a heating mat and will try it! The glass is still allowing some air exchange as I happen to see no condensation around the glass edges on the lid. Probably will work and solve my condensation issue. Just no SAB yet:confused:




I believe those mats certainly reach about 100-110F. I just checked it this afternoon, set at about half power and it reached 86F with a 20W heating mat.
What are you using to tape the dishes? It's probably a good thing tho, because that way you at least know that evaporation is happening and should eventually dry the plates in case of any excess condensation.
I believe parafilm allows for gas exchange by design.


Edited by Sandstone (11/03/20 12:22 PM)


Extras: Filter Print Post Top
OfflineMushRock
Stranger
Male User Gallery


Registered: 10/20/20
Posts: 40
Loc: The netherlands, Utrecht
Last seen: 3 years, 1 month
Re: Glass petris [Re: Sandstone]
    #27019314 - 11/03/20 12:31 PM (3 years, 2 months ago)

yeah I probably cheated somewhere as I don't have used any tape. I've put my active petri's inside of a small carton box, closed. And is stored inside a with closed with closed doors. The non actives are stored in open air without tape.
Like to watch during the day while working:laugh:


Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 22 hours
Re: Glass petris [Re: Sandstone]
    #27019317 - 11/03/20 12:33 PM (3 years, 2 months ago)

Well,
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, no color just because, 500mL bottled water.
Let the LME disolve in the water for 30-60 min, comes out really clear.
Simmer with agar and then PC 20 min. Tighten foil covered lid as soon as safety valve drops.

Like a sous vide I tried a crockpot at 130F and I pour around 117F.
Interesting thing is the water cools the agar quicker than air, then
the 130F holds it till yer ready to pour.

21 plates, 6 stack ok, but heavy to pour, made for a shaky hand. (also I'm old)
Enter in Agar Agar Sheet, I keep Excel sheets and keep forgetting my pours.
Mainly to track how many auto contams a pour eventually got.
And auto contam or emaculate contamination means you go to your plates
later and there's contam all by it's lonesome!  :lol:

Poured 25, @ 2:10 (added 4 plastic plates for long term fridge)
Wrap bottom plate of stacks, wrapped 2:50
I had a conversation where we felt leaving the plates unwrapped
might have been part of my auto contams, so I wrap within and hour.

Oh, wrap your plates with cling wrap.



             


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Edited by Inthepit (11/03/20 12:41 PM)


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: MushRock]
    #27019327 - 11/03/20 12:41 PM (3 years, 2 months ago)

Wow never imagined you could leave an active petri unwrapped..cool.
So all of the sterile, aseptic, still air or even flowhood jazz is compressed into about two steps and then you can just leave it without tape, glass lids fitting like shit and nothing happens:mushroom2: :laugh:


Extras: Filter Print Post Top
InvisibleSandstone
Cosmonaut

Registered: 09/23/20
Posts: 88
Re: Glass petris [Re: Inthepit]
    #27019331 - 11/03/20 12:44 PM (3 years, 2 months ago)

Quote:

Inthepit said:
Well,
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, no color just because, 500mL bottled water.
Let the LME disolve in the water for 30-60 min, comes out really clear.
Simmer with agar and then PC 20 min. Tighten foil covered lid as soon as safety valve drops.

Like a sous vide I tried a crockpot at 130F and I pour around 117F.
Interesting thing is the water cools the agar quicker than air, then
the 130F holds it till yer ready to pour.

21 plates, 6 stack ok, but heavy to pour, made for a shaky hand. (also I'm old)
Enter in Agar Agar Sheet, I keep Excel sheets and keep forgetting my pours.
Mainly to track how many auto contams a pour eventually got.
And auto contam or emaculate contamination means you go to your plates
later and there's contam all by it's lonesome!  :lol:

Poured 25, @ 2:10 (added 4 plastic plates for long term fridge)
Wrap bottom plate of stacks, wrapped 2:50
I had a conversation where we felt leaving the plates unwrapped
might have been part of my auto contams, so I wrap within and hour.

Oh, wrap your plates with cling wrap.



             



That is so awesome, thanks alot.
Yeah I was going to ask this at some point because I must've forgot and probably not going to find that post again; is it better to wrap with clingwrap foil than parafilm, why so?

:mushroom2::rocket:


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds High THC Strains   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Glass Petri's Or Plastic?? ShroomInduced 2,547 13 03/29/06 08:41 PM
by ShroomInduced
* [OT] - Where can we find a cheap seller of glass petri dishes in Europe? FreeSporePrints 950 4 04/15/06 01:33 AM
by FreeSporePrints
* Looking for glass petri dishs Brainiac 1,324 5 10/06/06 04:36 PM
by Prisoner#1
* Glass Petris Need more!! Which brand tahoe 462 5 12/09/07 10:07 PM
by tahoe
* how long to steralize glass petris in oven? Gr0wer 944 3 04/29/04 06:55 PM
by Gr0wer
* what generation am i on? mushsage 933 9 01/15/08 02:09 PM
by poot
* Cheap alternative to glass petri dishes at wally world *pics* XTCollection 8,442 17 06/30/19 06:28 PM
by Outdoor-Grower

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
693 topic views. 26 members, 191 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.01 seconds on 16 queries.