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Offlinechrisdab
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Registered: 04/06/04
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Lazy Mofo Bag Tek with WBS
    #2701783 - 05/19/04 04:21 PM (12 years, 6 months ago)

Are there greater contamination problems with using WBS and colonized popcorn in a ziplock bag with needle holes punched in? Bag will be placed in tyvek envelop then put in incubator. I believe using BRF has a less possibility for contamination but does that make it the only substrate that can work with this bag tek?

thanks


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OfflineFallenShroom
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Re: Lazy Mofo Bag Tek with WBS [Re: chrisdab]
    #2701796 - 05/19/04 04:24 PM (12 years, 6 months ago)

How are you going to sterilize your wbs ziplock bag ?


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Offlinechrisdab
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Re: Lazy Mofo Bag Tek with WBS [Re: FallenShroom]
    #2701813 - 05/19/04 04:28 PM (12 years, 6 months ago)

U sterilize in jars first then put them into a ziplock back with the jar of colonized substrate.


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Offlinemycoguy
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Re: Lazy Mofo Bag Tek with WBS [Re: chrisdab]
    #2702126 - 05/19/04 05:56 PM (12 years, 6 months ago)

what he means is....the bags may contain traces of contamination from say...the factory.

I tried the Lazy Mofo Bag Tek with BRF, and it didn't colonize one bit. I'm guessing that the pin holes didn't provide for enough oxygen. It didn't contam though, and i didn't take any extra steps to sterilize the ziplock bags. After a few weeks, I even tried poking more holes in the bags, but still saw no growth, so i just tossed the bags out.


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InvisibleDieCommie
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Registered: 12/11/03
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Re: Lazy Mofo Bag Tek with WBS [Re: chrisdab]
    #2702264 - 05/19/04 06:33 PM (12 years, 6 months ago)

Not a good idea i say....if you pour sterilized WBS out of a jar and into a bag....its no longer sterile


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OfflineKaptKid
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Re: Lazy Mofo Bag Tek with WBS [Re: chrisdab]
    #2702921 - 05/19/04 10:00 PM (12 years, 6 months ago)

Maybe a glove box
:goodluck:


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Offlinechrisdab
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Re: Lazy Mofo Bag Tek with WBS [Re: KaptKid]
    #2703656 - 05/20/04 12:43 AM (12 years, 6 months ago)

Yes you will need to do this in a sterile enviroment IE a glove box, since this is a G2G transfer. I have also heard of success stories using the bag from the factory, and since plastic isnt a medium for contamination growth I am confident in taking that risk.

I am just wondering if anyone has reported any successes using WBS in this bag tek.


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