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Offlinegabbk
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Oyster spawn with red metabolites?
    #27017739 - 11/02/20 03:28 PM (3 years, 4 months ago)

Hey guys.
I made a bag of oyster spawn doing g2g and everything seemed to be okay. It colonized quick enough to be considered healthy I think: I inoculated 300 grams of spawn from a jar to a 1700 grams grain bag and it colonized in 9 days, but looking it today I found two areas with red metabolites:

Is it okay to birth it with wood pellets anyways or should I throw it and save time?


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Edited by gabbk (11/02/20 03:29 PM)

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Offlinesendmehummus
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27017773 - 11/02/20 03:56 PM (3 years, 4 months ago)

Looks 100% fine. Spawn it upppp.

Edit: on second thought, it took some deeply focused zooming in to find that spot.. Id wait for another opinion, but the bag does look extremely healthy (maybe just a tad over hydrated).


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Be patient and keep it simple.

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Offlinebogdancev
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Re: Oyster spawn with red metabolites? [Re: sendmehummus]
    #27018454 - 11/02/20 10:29 PM (3 years, 4 months ago)

I cannot see any red areas. Everything looks fine to me.

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OfflineRogerRabbitM
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27026089 - 11/07/20 04:21 AM (3 years, 4 months ago)

Quote:

gabbk said:

I found two areas with red metabolites:

Is it okay to birth it with wood pellets anyways or should I throw it and save time?




The mycelium isn't going to grow thick enough against the edge of the plastic bag to cover the grains.  It's fine.
RR


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Offlinegabbk
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Re: Oyster spawn with red metabolites? [Re: RogerRabbit]
    #27037131 - 11/13/20 01:53 PM (3 years, 4 months ago)

The 3/11 (10 days ago) I made four 10pound bags, at 1:10 ratio with the spawn shown before, using pasteurized wood pellets lipa's way.
Are these spots normal?


:blush:

Edited by gabbk (11/13/20 02:07 PM)

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Offlinedeadmandave
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27037405 - 11/13/20 04:58 PM (3 years, 4 months ago)

those big chunks of mycelium? or the wet sawdust up top?

looks like the spawn couldve been mixed better. after you've pasteurized, take your time to really mix the spawn well. you can hand mix it in the cooler or bucket you pasteurized in. it will speed up colonizing and reduce the chance of contamination.

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Offlinegabbk
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Re: Oyster spawn with red metabolites? [Re: deadmandave]
    #27037466 - 11/13/20 05:32 PM (3 years, 4 months ago)

I was referring to that chunk of mycelium located exactly in the middle of the picture that has a center that turned more brown.

It's very strange you mention that, because I took my time mixing it. I put all the spawn at a big table and mixed the pasteurized pellets with the spawn really well for like for 10 minutes. Now I think it's the substrate. Its a soft wood.
This very wood is working really well on a reishi that I'm doing right as we speak. Also going to use it for lion's mane this weekend. But it seems really weird that it's giving me problem with oysters.
I had a couple oyster grows (in fact every one that I had) that took 6 weeks to start fruiting. And 2 weeks between flushes.


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Offlinegabbk
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27037883 - 11/13/20 10:49 PM (3 years, 4 months ago)

I think I've addressed the issue!!!
It seemed to lack GE. I went and made DEEP holes with the scalpel. All this time I've been making superficial holes.
When I stabbed these blocks, a few emanated somewhat recognizable anaerobic bacteria smell.
Next time I'll poke deep holes from birth:dancer:
What's a good way to introduce these holes? (Without using the scalpel as I did)


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InvisiblebodhisattaMDiscordReddit
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27040350 - 11/15/20 11:01 AM (3 years, 4 months ago)

The holes are not supposed to be anything but superficial. Mycelium transports oxygen

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OfflineHaywire
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Re: Oyster spawn with red metabolites? [Re: bodhisatta]
    #27040446 - 11/15/20 12:04 PM (3 years, 4 months ago)

to make a lot of holes I made something resembling a pastry perforator with thumbtacks on.
If you make one with 3 or 4 tacks next to eachother, you can quickly roll up and down the block, perforating the plastic and supplying the mycelium with a lot of breathing holes
don't forget to perforate the bottom as well so excess water can drain from substrate.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches

Edited by Haywire (11/15/20 12:05 PM)

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Offlinegabbk
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Re: Oyster spawn with red metabolites? [Re: Haywire]
    #27061988 - 11/28/20 05:59 PM (3 years, 3 months ago)

I'm definitely going to make that device, thank you a lot! That'd be super useful. One detail about that, if I organize the bags in layers (grain-pellets-grain-pellets and so on), would it make any difference if I poke the grains or substrate? I've seen a 'tek' on youtube distributing the layers that way and poking onto the grains with that very device you suggested Haywire. Also, this very same tek suggested to hydrate the wood pellets with cold water and white vinegar at 5% volume. I emulated exactly that tek with lion's mane and by the seventh day it COMPLETELY turned green by trich. It was really gross. What did it happen there? Was it the vinegar? Or the fact that I poked the spawn itself?


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Edited by gabbk (11/28/20 06:19 PM)

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Offlinegabbk
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27062006 - 11/28/20 06:10 PM (3 years, 3 months ago)

And here another question, related to the last pic I posted about some spots in the bags. Here's another bag that has the same thing but they are more clear to the eye.

I'm sure we'll all agree it's contamination. What is it exactly? I threw a bag that was full of these greenish spots, but before I torn it apart and smelled it, and realised it was really strong acidic/sour, and to the touch felt like.. kind of a goo.


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OfflineHaywire
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Re: Oyster spawn with red metabolites? [Re: gabbk]
    #27062354 - 11/29/20 01:28 AM (3 years, 3 months ago)

I like to mix before I fill but layering worked as well.
I never thought of the issues that could arise with perforating the spawn.
colonized grains should still be somewhat resistant to contamination.

I've never used vinegar in substrates.
I suppose you use that to lower pH which makes the substrate less ideal for contaminants?
If you apply proper sterilization or pasteurization techniques then there is no need for that.
and to be fair, it would be best you get these techniques down if you want to continue in this hobby.

you can hydrate pellets with cold water but it just takes much longer to expand.

it's definitely contaminated. usually when I get those spots and lines, it's trichoderma for me. but if you claim the spots to be goo-like, then my guess would be bacterial.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches

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OfflineSHROOMSISAY01M
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Re: Oyster spawn with red metabolites? [Re: Haywire]
    #27062386 - 11/29/20 02:58 AM (3 years, 3 months ago)

The inside of the grain is not colonized so try not to break the grain.

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OfflineHaywire
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Re: Oyster spawn with red metabolites? [Re: SHROOMSISAY01]
    #27063997 - 11/30/20 05:26 AM (3 years, 3 months ago)

High tech tool!



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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches

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