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OfflinePharmacien
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Registered: 11/02/20
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LC - blood testing the truffle , (maybe a dumb question)
    #27017499 - 11/02/20 01:09 PM (4 years, 2 months ago)

Hello shroomerians ,

    Sorry if my question have already be treated, i tried to search in many ways but didn't found a specific answer so :

    I  got some sclerottes and I ask myself if I could sterilize a jar with LC liquid with a silicone sealed hole , and a sterilized syringe also filled with LC liquid , so I would rub the sclerotte with 70 alcohol , and then poke the sclerotte with the syringe taking some tissue from the center of the sclerotte then putting it inside de jar (all this process flamming the needle) .

    The problem is : I don t have a sab where I live as all my material is with my parents and my spores and s-syringues are there .
    I thought also if I could not just suck the tissue inside de syringe and wait for mycelium to form there .
    Also, is the inner tissue of the sclerotte too hard for a normal syringe?

My goal is just to successfully make a LC syringe and not go any further without a sab for the moment .

          Thank you guys in advance ,

:boofgirl:  :tryingnottodie:  :doingmediocre:

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InvisibleJosex
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Re: LC - blood testing the truffle , (maybe a dumb question) [Re: Pharmacien]
    #27021131 - 11/04/20 10:12 AM (4 years, 2 months ago)

That's going to fail 99% guaranteed.
Everybody can make a SAB. You can take inner tissue from the truffle using a scalpel and transfer it to agar and make LC with clean growth from agar.

Alternatively, if you don't have a scalpel, you can look up the last link in my sig. Squirt the tissue in a sterilized PF tek puck, let it grow out, maybe tranfer a couple times and when growth appears clean you can poke the puck to make LC.

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OfflinePharmacien
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I'm a teapot


Registered: 11/02/20
Posts: 65
Last seen: 3 years, 10 months
Re: LC - blood testing the truffle , (maybe a dumb question) [Re: Josex]
    #27021158 - 11/04/20 10:24 AM (4 years, 2 months ago)

ok I'll wait and do clean on agar so , thank you

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