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OfflineKabluee
Stranger

Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: EZPZyaBZ]
    #27145043 - 01/13/21 04:52 AM (3 years, 15 days ago)

Quote:

EZPZyaBZ said:
Yo bro, since you are in a position to equip yourself so thoroughly "like it ain't no thang", you should get an AA 75x when you can. You will LOVE it.

They are plug-in sterilizers and you can set them up with a timer. If you are planning on going relatively big it will change your life.

After my flowhood, it's the best thing I own. Might be close actually.




I definitely thought about getting that exact PC but at the end of the day the cost of it was too much at the time and the PC i currently have is sufficient for the time being but i will definitely be getting one some day in the future! πŸ„πŸ™


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OfflineKabluee
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Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27145066 - 01/13/21 05:12 AM (3 years, 15 days ago)

One question if anybody can answer;

My PC has a release valve set at 24psi that automatically releases and holds at that pressure.
Is this too high pressure for PCing agar or grain?
(Ive currently been releasing pressure manually through the other release valve when around 17psi)

Thank youπŸ„


Edited by Kabluee (01/13/21 06:06 AM)


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OfflineKabluee
Stranger

Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27148151 - 01/14/21 04:56 PM (3 years, 14 days ago)

So ive decided to go with Corn instead of Rye as i have a few bags of the rye currently colonizing and im finding it difficult to see if they are clean or not due to the color of rye being brown and mold and contams tend to be green which makes it very difficult to see being colorblind,so im thinking corn will give me more transparency when looking for contams.


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InvisibleEZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27148890 - 01/15/21 12:35 AM (3 years, 13 days ago)

Quote:

Kabluee said:
So ive decided to go with Corn instead of Rye as i have a few bags of the rye currently colonizing and im finding it difficult to see if they are clean or not due to the color of rye being brown and mold and contams tend to be green which makes it very difficult to see being colorblind,so im thinking corn will give me more transparency when looking for contams.





I will leave the discussion of rye vs corn for someone else, but if you are relying too much upon your eyes in this hobby you are going to be let down a fair bit. Your flowhood with proper sterile technique is the answer. Rather than choose a grain based on something like "easier to see contaminants on", I'd go with what you find to be the better performing grain in general, because even if the grain was see through, you aint gonna see all of the contaminants that can and will rear their ugly heads if you don't have sterile technique down.  Of the two you mentioned my money is on rye though, and I think that sentiment is pervasive, but popcorn does have it's fans. Do what works for you, but don't give too much credit to any particular grain when it comes to successful grows, or to being able to see stuff on it, or because of the contamination rate you may or may not experience, or because of anything else, because grain choice is a distant second to the end all be all of sterile technique. I may already be preaching to the choir to some extent as you've clearly done your homework broskie. You're on your way, I expect big things. :smile:


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OfflineKabluee
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Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: EZPZyaBZ]
    #27149200 - 01/15/21 06:40 AM (3 years, 13 days ago)

I dont expect to have to rely on it but again being colorblind makes it difficult to see contams on brown surfaces for me,actually almost impossible. atleast with corn i will have some fighting chance to see things as needed. I also have not been able to find rye in my local area so im definitely going to give corn a shot and if it turns out to be unfavourable i will give oats a try

thanks for the input buddy! Mush luvπŸ˜ŽπŸ„


Edited by Kabluee (01/15/21 06:42 AM)


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 46 minutes
Trusted Cultivator
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27149419 - 01/15/21 09:25 AM (3 years, 13 days ago)

There is no grain when prepped and sterilized properly that will harbor contaminates. Corn works great. Oats work great. Rye, wheat, barley, tritacle, millet, milo, sorghum, spelt etc all work great.

Get a microscope for sure.


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleEZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27149628 - 01/15/21 11:51 AM (3 years, 13 days ago)

I haven't used a microscope since my schooling days and wouldn't know where to start. I want to get one as it has been discussed a fair bit recently, do you have any recommendations? What kind of specs should one look for on a microscope meant for this endeavor?


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OfflineKabluee
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Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27151151 - 01/16/21 09:45 AM (3 years, 12 days ago)

Yea definitely will be getting a microscope. I currently have a 400x zoom microscopic lense which works ok for taking photos but want a microscope asap for sureπŸ„


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 46 minutes
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Re: Building Home Lab and Cultivation Room [Re: Kabluee] * 1
    #27151343 - 01/16/21 11:52 AM (3 years, 12 days ago)

This is looking through the lid of a petri dish at 1600. Photos are taken with a 5mp microscope lens camera. I would recommend any good BINOCULAR microscope preferably with both bottom and top lights.



The field of view is about 10x that of the camera with the naked eye and real life resolution is clear as day.

This is an oil immersion view of zapotecorum? spores.




I see LOTS of mis-identification due to lack of microscopy capabilities. Very experienced cult members regularly call things mold and bacteria that are absolutely not either of those things... (picture 2 is Aspergillus Oryzae mold)


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleEZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27151417 - 01/16/21 12:36 PM (3 years, 12 days ago)

Quote:

Nichrome said:
I would recommend any good BINOCULAR microscope preferably with both bottom and top lights.





Thanks Big Dogg :cheers:


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OfflineMycoJones
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Registered: 11/29/20
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Re: Building Home Lab and Cultivation Room [Re: EZPZyaBZ]
    #27151611 - 01/16/21 02:36 PM (3 years, 12 days ago)

Great setup, great genetics, great thread. Very interested to see these Enigma cultures fruit.

Curious, does the chinese laminar flow hood come with a pre-filter?


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OfflineKabluee
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Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: MycoJones]
    #27151659 - 01/16/21 03:02 PM (3 years, 12 days ago)

ThanksπŸ„ yes pre filter was included,but i checked recently and the price of the unit has more than doubled!

I knocked up 12 jars of corn with Enigma LC today will keep you guys updated! Mush luv


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OfflineKabluee
Stranger

Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27152790 - 01/17/21 06:19 AM (3 years, 11 days ago)

Quote:

Nichrome said:
This is looking through the lid of a petri dish at 1600. Photos are taken with a 5mp microscope lens camera. I would recommend any good BINOCULAR microscope preferably with both bottom and top lights.



The field of view is about 10x that of the camera with the naked eye and real life resolution is clear as day.

This is an oil immersion view of zapotecorum? spores.




I see LOTS of mis-identification due to lack of microscopy capabilities. Very experienced cult members regularly call things mold and bacteria that are absolutely not either of those things... (picture 2 is Aspergillus Oryzae mold)




Awesome! Nice pictures,Thanks for the info buddy πŸ„


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OfflineGypsyBastard
Misanthrope
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Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27152948 - 01/17/21 08:22 AM (3 years, 11 days ago)

That is the one thing I wish I had was a binocular scope. You are spot on about the top and bottom lights and a dimmer is useful too but man after a while, trying to hold one eye closed gets weird. I had the same problem with astrophotography. Those are muscles that you don't use like that often and they get tired and spastic when you force them to contract for long periods repeatedly.

With that said, a monocular scope is better than no scope and I still enjoy using mine.


Edited by GypsyBastard (01/17/21 08:23 AM)


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 46 minutes
Trusted Cultivator
Re: Building Home Lab and Cultivation Room [Re: GypsyBastard]
    #27152993 - 01/17/21 08:44 AM (3 years, 11 days ago)

Dimmer! very important. Good call.

My scope is binocular with 4 mag settings but it is only bottom lit and dimming. All my top lit viewing and photos are using a handheld flashlight next to the scope. It is really awkward with a flashlight.

The one I use was about 350 USD and came with a digital camera.


At least I don't have to melt, pour, and polish lenses like they did a couple hundred years ago...


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Edited by Nichrome (01/17/21 08:48 AM)


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OfflineGypsyBastard
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Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27153264 - 01/17/21 11:23 AM (3 years, 11 days ago)

I don't know where to file is so had to screen shot it from my phone's lock screen. This is the edge of a cut in the agar where I removed a wedge and the mic started to grow out from. It was taken with my cell phone held up to the scope so again visually it was much clearer.


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InvisibleEZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27153285 - 01/17/21 11:32 AM (3 years, 11 days ago)

Like the new JC avatar Nichrome :yesnod:


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 46 minutes
Trusted Cultivator
Re: Building Home Lab and Cultivation Room [Re: EZPZyaBZ]
    #27153360 - 01/17/21 12:03 PM (3 years, 11 days ago)

Thanks dude.

Circa 1986. Dude is one of my all time favorite people who ever existed. Humor is one of the highest forms and that guy was a master.


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineKabluee
Stranger

Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Nichrome]
    #27153775 - 01/17/21 03:49 PM (3 years, 11 days ago)

Deleted


Edited by Kabluee (01/17/21 05:23 PM)


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OfflineKabluee
Stranger

Registered: 10/03/20
Posts: 210
Last seen: 2 months, 4 days
Re: Building Home Lab and Cultivation Room [Re: Kabluee]
    #27153919 - 01/17/21 05:22 PM (3 years, 11 days ago)

Enigma LC turned out to be clean so that was added to 6 quart jars of sterilized corn to colonize, along with 4 agar plates in addition into two more quart jars of corn for a total of 8 quarts of Enigma on corn and 6 quarts of Enigma on rye.

APE has been transfered onto new plates for a hopefully nice and clean T2! πŸ„



Edited by Kabluee (01/18/21 02:48 AM)


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