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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 15 days, 5 hours
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Proud of my first clean spawn?
#27016506 - 11/01/20 10:05 PM (3 years, 2 months ago) |
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So I did my first run with the PF tek and got a very tiny but existent first flush the other day.
I started with agar and grain alongside the PF tek since I could tell this is something I was going to be more interested in.
I streaked spores, and made a transfer to a pasty plate. You can see the tiny spot at about 5 O' clock that I took the transfer from.

Here is the plate. I don't think there was any contam on this plate. The mycelium didn't colonize as quickly over the indented area, but there was liquid water over that spot while it was colonizing. The water stayed clear. It also colonized further than this after I took the pic and continued into the indented area - I didn't A2g the same day as this photo.
 
I Cut the pasty plate into about 9 pieces with a flamed scalpel, and "ti tac toe dropped" it into a pint jar.
I saw some colonization and shook it at about what I would call 10%.
I then saw fluff over EVERYTHING after about 3-5 more days. I saw some obvious rhizomes,and a ton of white fluff. I really wasn't sure if it was all mycelium fluff or some was mold. When I shined a bright light it looked pure white, but it was still very wispy and not anywhere close to as "strong" looking as the mycelium fully on the grains.
I knew I should have waited, and I didn't get any photos, but I gave it a big shake anyway, and it's been another 5 days or so. I only see white mycelium, and it's all coming straight out of the grains. I see almost nothing that's fluffy, but I do see it slowly coming out of the oat hulls and it's definitely crawled on any exposed grains. It is still moving every day.
Is this clean spawn, or is this dead spawn that got killed by mold? If it were mold, wouldn't that "recover" much faster than mycelium and I would have it again?
Also, does it look bacterial?
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Panaeolus Bisporus
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the man
still masked



Registered: 08/12/99
Posts: 6,681
Loc: C A N A D A
Last seen: 2 hours, 47 minutes
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Re: Proud of my first clean spawn? [Re: karri0n]
#27016534 - 11/01/20 10:25 PM (3 years, 2 months ago) |
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give it a week and see what happens no need to shake at this stage.
soak or boil oats?
polyfill for gas exchange?
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 15 days, 5 hours
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Re: Proud of my first clean spawn? [Re: the man]
#27016846 - 11/02/20 06:49 AM (3 years, 2 months ago) |
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I boiled oats for 20 mins, then dried them on mesh strainers for 2 hours. I've got a few jars just sitting, never opened, to see if they clump up/mold/liquefy/rot/etc, and this one inoculated. The quart jar looks super nice and I've got a gorgeous grown out T2 which will get a2g from it.
I'm using three layers of micropore for GE over a 1/4" hole on this one.
I haven't tried polyfil yet. I've been using the quarter-sized SFD's for the pp5 mason lids, and holes with micropore for shit like pickle/jelly jars.
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Panaeolus Bisporus
Edited by karri0n (11/02/20 06:53 AM)
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