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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Quote:
Mnemosynea said:
Quote:
p9hu7 said: Shooting spores into a spawn bag is more like an F- for technique. I would go as far as to say that it's asinine. If you can run bags then why can't you make agar.
Obviously. Read the thread. The whole point of these is NOT for producing optimized results. By technique in that context I was referring to sterile environment for inoculation. OP Did use agar.
You are missing the point. The method of inoculation is part and parcel to sterile technique, if you use a spore syringe to noc up a spawn bag you may consider your technique to be non sterile and therefore garbage.
Proper technique would have followed a chain of sterile procedures like:
Spores cleaned on agar>agar wedge used to make either LC,LI, or wedge to grain for grain master> use LC LI, or gtg with the master.
Shooting spores into grain and then asking why you couldn't grow anything is a giant 
Using pre sterilized bags that have been shipped in the mail and then left to sit is equally prone to failure.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Theres obviously something going wrong if "all the innoculants and grain bags were perfect" everything was totally right but nothing grew... hmmm... seems lime something wasnt right if there was that many bags bougjt that were from a "reputable" vendor and it wasnt the innoculant or technique then that vendor isnt reputable. Theres always people on here saying " I did everything right but my shit fucked up?" Obviously you didnt do everything right
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
Edited by TedsDead (11/02/20 06:44 PM)
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Mnemosynea
Trying to Remember


Registered: 10/23/20
Posts: 52
Last seen: 2 years, 6 months
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Quote:
p9hu7 said: Shooting spores into grain and then asking why you couldn't grow anything is a giant 
I wasn't asking, neither was OP. The question was about the viability of the grain bags themselves. With as many different ways as OP attempted to grow something on this grain, it's fairly obvious that after having sat for 2+ months they were no longer viable.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Yeah, that's why I quoted you, and not the OP.
Quote:
p9hu7 said:
Quote:
Mnemosynea said: I was using proven spores and A+ technique.
Pretty sure he mentioned using spores, LC, and g2g....so it's still relevant to the OP, especially that last part about using questionable spawn bags
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Nothing is adding up still... even if the bags sat for that long the OP says there were no signs of contamination. Just no growth... ive let bags sit for that long before nocing and theyve been fine. I attribute it to technique... or they are just not trained at recognizing contamination
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Grain Bag Issue [Re: TedsDead]
#27018095 - 11/02/20 06:54 PM (3 years, 2 months ago) |
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I agree, what we've mentioned in our responses covers the gambit. It could be any of the above...from poorly sterilized/compromised bags, to dirty spore syringe and poor technique or bad spawn....or all of the above.
The only way to truly know is to deconstruct. Assume it's a little bit of everything. Use fresh properly sterilized bags. No spore syringes, ensure proper technique to produce clean spawn.
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Surfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 18 days
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So since I received my pressure cooker I have been making my own grains and doing agar work and all is going great. I had 12 bags laying around that I had no real use for since I was doing jars in my PC now. So I decided to use them before they got too old cause the website said use within three months If I remember correctly. They were about a month old from when I received them but I have no idea how old they actually were since I bought them from an online source.
I did put spores on agar and clean them up. I also used a piece from the center of mushroom stem from a previous grow and put that to agar and grew that out and cleaned it up. I also put a piece of clean agar into a LC solution and grew that out. Before I used the LC I put it on agar and made sure it was clean.
I have used the agar and LC to inoculate the grains I made myself and they came out great. I took some of those grains as well and put them in the bags and nothing happened but I also put them in the jars I made and they grew out great.
Yes, the spore syringes I used on the bags was a roll of the dice and more or less me just seeing what would happen. My first ever grow was a ms syringe grow in a 65 qt mono and although it did eventually contam with the green, I got one huge flush out of it. If anyone cares to see it I have a thread with the grow back in July. It was super fun being my first grow. Learned a lot from you guys during it.
Anyway, I know better now then to use a ms syringe when I can just use agar first, but I had so many bags I was just curious as to which would colonize faster and was really just experimenting.
I just think I would see some growth in at least one of the 12. I mean, even contamination growth. A few of the bags after a month got like wet looking inside but that was about it.
I don’t know. Maybe they were just too dry inside.
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Surfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 18 days
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Well, I just saw on their website that their grain bags are only good for 4-5 weeks at room temperature. I had then for about 3.5 - 4 weeks before I used them so I’m guessing they were just too old. Maybe they dried out? I don’t know but I guess that’s what it was.
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