Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Offlinewxorx
elsewhere
 User Gallery

Registered: 10/18/19
Posts: 90
Last seen: 10 days, 9 hours
advice needed
    #27015534 - 11/01/20 10:56 AM (3 years, 3 months ago)

Hello everyone,
I am new to cultivation and still have to grasp all the terminology, teks and whatnot, so please bear with me.

I am still reading and learning slowly, and hopefully soon will dive into my first experiments with agar.
I still need to buy myself a pressure cooker, some kitchen scale, petri dishes are on it's way already. SAB is the next thing to build.

I have only attempted once to get store bought pleurotus growing mycelium on a wood chips in a plastic bag on my terrace.
I really didn't knew what I am doing, I just put some pieces from the base of the stems in a polyetilene bag with
moist woodchips, hoping that there might be some mycelium that would spread over the wood. That worked actually, but I didn't get them to fruit.
Next experiment was with wild found Panaeolus antillarum - there I prepared a substrate from some dried horse dung,
vermiculite and dead grass debris (approx. 4:2:1 ratio) and tap water, which I pasteurized in a microwave for ~15 minutes in a plastic
PP5 box from ice cream. The ratios I chose where random, I somehow felt they maybe should work.
Again, I put there small piece of stem base in the box and left that on top of my fridge. Some time later (~1.5 weeks maybe)
I noticed some delicate white cobweb started to cover the substrate. I opened the box, moisted it and left it on the terrace.
The cobweb disappeared. Long story short - I put the box back inside on the top of the fridge. It sat for a week maybe, then suddenly
one day I noticed a pin. It developed in just 3-4 days to a single nice long white mushroom. The very same is on my avatar now.
I know I never followed any proven sterile tek, but I liked to experiment with what I have, and with available time I have.

That was like an introduction, hopefully not too long and boring.

Now to the real question..

Recently I found this, and was very excited when it was
possibly identified as a P.Serbica. I've made a spore print, and keep it for the near future when I will catch up working with agar.

But again, I was eager to try to spread any possible mycelium left on the stem base to spread over some nutrient media.
This time I chose dry broadleaves, pieces of wood branches and a pinch of starch, which I put on a PP5 container with tapwater,
pasteurized in a microwave (I don't have a PC), let it cool and then cut and put the stem base of that mushroom in that container.



Now I believe that I see fine whitish cobweb developed there.. It might be anything else, even mold, but it could be the real thing..

The questions are now:

When is it safe to open the container? I know the conditions might not be optimal, but what can I do to keep this mycelium healthy
and alive in order to make my own outdoor patch in the future in a suitable place in the forests nearby?
Should I tranfer it to another media? Should I keep it like this? What temp would be best? Moisture..
Anything else?

I feel now like I have a piece of gold, but fragile and volatile. And I am afraid to lose it. What should I do to make it live and spread it?

Thank you for all suggestions.


--------------------
void **


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,776
Loc: A thought
Trusted Cultivator
Re: advice needed [Re: wxorx]
    #27015551 - 11/01/20 11:05 AM (3 years, 3 months ago)

Your attempts to sterilize anything in a microwave are futile, especially when you are just dropping a piece of dirty wild picked stem into it. You, I think already know the answer, get your agar game going. People dont just do it for fun like there is another reliable way to go about it. Its a necessity if you want to play with mycelium with any more than a piss in the wind chance of things working. Cool that you lucked out on the first one though!


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Offlinewxorx
elsewhere
 User Gallery

Registered: 10/18/19
Posts: 90
Last seen: 10 days, 9 hours
Re: advice needed [Re: Smartattack]
    #27015609 - 11/01/20 11:50 AM (3 years, 3 months ago)

Thanks, sure, I know microwave does not sterilize, I did that in a hope that any foreign spores, myc or bacteria already in the substrate will get less chances, and not what the dirty wild picked stem will bring there. Yes, I know agar is the way to go, but the question is what if the mycelium I have is the right one and it is not contamination or anything else. Should I do something more to make it progress better and not to lose it?


--------------------
void **


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,776
Loc: A thought
Trusted Cultivator
Re: advice needed [Re: wxorx]
    #27015621 - 11/01/20 11:57 AM (3 years, 3 months ago)

If it by chance grows healthy then just leave it in there. Once you get agar plates, you can transfer a bit to keep the culture.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Offlinewxorx
elsewhere
 User Gallery

Registered: 10/18/19
Posts: 90
Last seen: 10 days, 9 hours
Re: advice needed [Re: Smartattack]
    #27015637 - 11/01/20 12:05 PM (3 years, 3 months ago)

Thank you, I will wait to see how it goes. In any case I will update later how it went.


--------------------
void **


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* straw questions and other bits of advice needed Barbi 1,247 4 04/22/02 02:31 PM
by Barbi
* some casing advice needed.... gratefulredhead 984 2 05/14/01 10:30 AM
by gratefulredhead
* Post deleted by Moe Howard bigidiot 916 9 07/31/01 11:57 PM
by BrownPastures
* Experimenting with substrates...? *DELETED* TheCaptain 835 2 10/03/01 05:02 PM
by TheShroomster
* ???Need Some ANSWERS About Substrate??? SwShroomr 634 6 02/26/02 10:21 AM
by hispeed67
* NEWBE... need most favorable spore-substrate YCpimp 1,244 4 02/07/02 06:48 AM
by tomatofarmer
* Advice needed wheelerd 639 1 11/12/01 06:13 PM
by phrozendata
* Sterilize casing or not?...Expert advice needed! bassplayer74 2,827 6 12/02/01 02:13 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
113 topic views. 29 members, 152 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.