Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Liquid Cultures, Substrate Bags   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Offlinesmalldaddy
Mushroom Enthusiast

Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 13 hours
Oat Jar Contamination Issues from agar
    #27015527 - 11/01/20 10:48 AM (3 years, 3 months ago)

Hello! I am having more issues with contamination in my oat spawn jars. I recently started using agar plates, and decided to use my first plate to inoculate some jars. This first plate did not have a single drop in the middle of the plate, but various drops of a spore syringe on it. I've read that I should have at least done one other transfer to agar to make sure it was not contaminated, however, would there be anything else causing this contamination in my jars? I used bod's SAB protocol, and am a trained microbiologist, so my sterile technique is not sloppy (always trying to improve though). I PC at 15psi for 2 hours for all my jars, and use an autoclave for my agar. The plates looked clean with white growth, but not inoculating one drop in the middle may have hidden some contamination?


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,776
Loc: A thought
Trusted Cultivator
Re: Oat Jar Contamination Issues from agar [Re: smalldaddy]
    #27015558 - 11/01/20 11:11 AM (3 years, 3 months ago)

Yeah its a total crapshoot to work from the germination plate. Think about it, the entire purpose of agar is to allow you to clean your culture. By putting liquid from a syringe into a plate, and then that plate into grain, you literally did exactly the same thing as putting the syringe directly to grain. All you did was you gave it an alternative place to germinate rather than the grain. And the contamination as well.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Offlinesmalldaddy
Mushroom Enthusiast

Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 13 hours
Re: Oat Jar Contamination Issues from agar [Re: Smartattack]
    #27015563 - 11/01/20 11:15 AM (3 years, 3 months ago)

Thank you for putting that in perspective! That's definitely my issue then, I will isolate this time before I transfer. I keep getting in a hurry trying to inoculate, but this process is certainly teaching me patience. Thanks for your help:smile:


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,776
Loc: A thought
Trusted Cultivator
Re: Oat Jar Contamination Issues from agar [Re: smalldaddy]
    #27015572 - 11/01/20 11:19 AM (3 years, 3 months ago)

Patience has probably killed 90% of the people who have tried this stuff. Once you get that down the rest is easy.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Liquid Cultures, Substrate Bags   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,090 21 04/30/02 03:27 AM
by Zebedee
* Contamination issues kykeon 1,160 1 06/13/02 01:31 AM
by Roger_irrelevant
* Re: MUSHROOM TRIVIA contaminations with agar/rye darkangel932 642 2 04/21/01 03:47 PM
by darkangel932
* Condensation in jars contamination? gonnadoit 1,522 1 07/01/02 05:20 AM
by DreaMaTrix
* Re: Green mold again! Agar experts help me! Anonymous 7,380 9 12/01/99 10:20 AM
by Anonymous
* ZAYParinoid: light and contamination Sugaree 2,033 10 03/24/02 05:10 PM
by ParticleMan
* Purple/Pink contamination can be DEADLY
( 1 2 3 all )
Ryche HawkV 32,545 42 04/23/22 11:05 PM
by Imatryin
* 100% Contaminent Free Cultivation tHeMaGe 1,623 6 11/12/01 11:38 AM
by amazinblue

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
137 topic views. 21 members, 149 guests and 45 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.007 seconds on 12 queries.