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Offlinesmalldaddy
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Registered: 12/03/19
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Oat Jar Contamination Issues from agar
    #27015527 - 11/01/20 10:48 AM (4 years, 2 months ago)

Hello! I am having more issues with contamination in my oat spawn jars. I recently started using agar plates, and decided to use my first plate to inoculate some jars. This first plate did not have a single drop in the middle of the plate, but various drops of a spore syringe on it. I've read that I should have at least done one other transfer to agar to make sure it was not contaminated, however, would there be anything else causing this contamination in my jars? I used bod's SAB protocol, and am a trained microbiologist, so my sterile technique is not sloppy (always trying to improve though). I PC at 15psi for 2 hours for all my jars, and use an autoclave for my agar. The plates looked clean with white growth, but not inoculating one drop in the middle may have hidden some contamination?

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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,788
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Re: Oat Jar Contamination Issues from agar [Re: smalldaddy]
    #27015558 - 11/01/20 11:11 AM (4 years, 2 months ago)

Yeah its a total crapshoot to work from the germination plate. Think about it, the entire purpose of agar is to allow you to clean your culture. By putting liquid from a syringe into a plate, and then that plate into grain, you literally did exactly the same thing as putting the syringe directly to grain. All you did was you gave it an alternative place to germinate rather than the grain. And the contamination as well.


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Offlinesmalldaddy
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Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 10 months
Re: Oat Jar Contamination Issues from agar [Re: Smartattack]
    #27015563 - 11/01/20 11:15 AM (4 years, 2 months ago)

Thank you for putting that in perspective! That's definitely my issue then, I will isolate this time before I transfer. I keep getting in a hurry trying to inoculate, but this process is certainly teaching me patience. Thanks for your help:smile:

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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,788
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Re: Oat Jar Contamination Issues from agar [Re: smalldaddy]
    #27015572 - 11/01/20 11:19 AM (4 years, 2 months ago)

Patience has probably killed 90% of the people who have tried this stuff. Once you get that down the rest is easy.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.

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