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Offlinewxorx
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Registered: 10/18/19
Posts: 90
Last seen: 1 month, 23 days
advice needed
    #27015534 - 11/01/20 10:56 AM (3 years, 2 months ago)

Hello everyone,
I am new to cultivation and still have to grasp all the terminology, teks and whatnot, so please bear with me.

I am still reading and learning slowly, and hopefully soon will dive into my first experiments with agar.
I still need to buy myself a pressure cooker, some kitchen scale, petri dishes are on it's way already. SAB is the next thing to build.

I have only attempted once to get store bought pleurotus growing mycelium on a wood chips in a plastic bag on my terrace.
I really didn't knew what I am doing, I just put some pieces from the base of the stems in a polyetilene bag with
moist woodchips, hoping that there might be some mycelium that would spread over the wood. That worked actually, but I didn't get them to fruit.
Next experiment was with wild found Panaeolus antillarum - there I prepared a substrate from some dried horse dung,
vermiculite and dead grass debris (approx. 4:2:1 ratio) and tap water, which I pasteurized in a microwave for ~15 minutes in a plastic
PP5 box from ice cream. The ratios I chose where random, I somehow felt they maybe should work.
Again, I put there small piece of stem base in the box and left that on top of my fridge. Some time later (~1.5 weeks maybe)
I noticed some delicate white cobweb started to cover the substrate. I opened the box, moisted it and left it on the terrace.
The cobweb disappeared. Long story short - I put the box back inside on the top of the fridge. It sat for a week maybe, then suddenly
one day I noticed a pin. It developed in just 3-4 days to a single nice long white mushroom. The very same is on my avatar now.
I know I never followed any proven sterile tek, but I liked to experiment with what I have, and with available time I have.

That was like an introduction, hopefully not too long and boring.

Now to the real question..

Recently I found this, and was very excited when it was
possibly identified as a P.Serbica. I've made a spore print, and keep it for the near future when I will catch up working with agar.

But again, I was eager to try to spread any possible mycelium left on the stem base to spread over some nutrient media.
This time I chose dry broadleaves, pieces of wood branches and a pinch of starch, which I put on a PP5 container with tapwater,
pasteurized in a microwave (I don't have a PC), let it cool and then cut and put the stem base of that mushroom in that container.



Now I believe that I see fine whitish cobweb developed there.. It might be anything else, even mold, but it could be the real thing..

The questions are now:

When is it safe to open the container? I know the conditions might not be optimal, but what can I do to keep this mycelium healthy
and alive in order to make my own outdoor patch in the future in a suitable place in the forests nearby?
Should I tranfer it to another media? Should I keep it like this? What temp would be best? Moisture..
Anything else?

I feel now like I have a piece of gold, but fragile and volatile. And I am afraid to lose it. What should I do to make it live and spread it?

Thank you for all suggestions.


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void **


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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,775
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Re: advice needed [Re: wxorx]
    #27015551 - 11/01/20 11:05 AM (3 years, 2 months ago)

Your attempts to sterilize anything in a microwave are futile, especially when you are just dropping a piece of dirty wild picked stem into it. You, I think already know the answer, get your agar game going. People dont just do it for fun like there is another reliable way to go about it. Its a necessity if you want to play with mycelium with any more than a piss in the wind chance of things working. Cool that you lucked out on the first one though!


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Offlinewxorx
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Registered: 10/18/19
Posts: 90
Last seen: 1 month, 23 days
Re: advice needed [Re: Smartattack]
    #27015609 - 11/01/20 11:50 AM (3 years, 2 months ago)

Thanks, sure, I know microwave does not sterilize, I did that in a hope that any foreign spores, myc or bacteria already in the substrate will get less chances, and not what the dirty wild picked stem will bring there. Yes, I know agar is the way to go, but the question is what if the mycelium I have is the right one and it is not contamination or anything else. Should I do something more to make it progress better and not to lose it?


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InvisibleSmartattack
C'mon man
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Registered: 12/21/18
Posts: 3,775
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Re: advice needed [Re: wxorx]
    #27015621 - 11/01/20 11:57 AM (3 years, 2 months ago)

If it by chance grows healthy then just leave it in there. Once you get agar plates, you can transfer a bit to keep the culture.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlinewxorx
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Registered: 10/18/19
Posts: 90
Last seen: 1 month, 23 days
Re: advice needed [Re: Smartattack]
    #27015637 - 11/01/20 12:05 PM (3 years, 2 months ago)

Thank you, I will wait to see how it goes. In any case I will update later how it went.


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