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OfflineFoTwenty
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Registered: 05/31/13
Posts: 639
Last seen: 2 months, 1 day
Help with agar sectoring.
    #27015198 - 11/01/20 07:10 AM (3 years, 2 months ago)

From which parts do you guys think it’s better for transferring ?. Should I wait another week for it to grow more? Thanks.




I apologize for the quality. This is  T5, the other ones are getting some metabolites on top of the myc in the. Plate... any thoughts why that happens?


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts



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InvisibleMateja
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Registered: 07/14/16
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Re: Help with agar sectoring. [Re: FoTwenty]
    #27015212 - 11/01/20 07:25 AM (3 years, 2 months ago)

Quote:

FoTwenty said:
From which parts do you guys think it’s better for transferring ?. Should I wait another week for it to grow more?



That's up to you to decide. If your culture is clean then there's no reason to keep transferring, grow it out more if you want a bigger culture and use it to inoculate something :thumbup:


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OfflineFoTwenty
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Registered: 05/31/13
Posts: 639
Last seen: 2 months, 1 day
Re: Help with agar sectoring. [Re: Mateja]
    #27015249 - 11/01/20 07:52 AM (3 years, 2 months ago)

Ahhh ok.  Thanks man. So isolates are from doing tissue clone...?
Then I will wait for it to grow more and make more transfer for master plates.
What do you prefer. Agar to jar and wait till it’s 100% and transfer  that jar to bags?
How many bags can a quart do well with minimum bag shaking, I notice bags should be shaken 2 times minimum. The seal from the impulse sealer, Has sharp edges and the more you shake it gives me stress points in the seal, making micro holes...
Or agar plate sliced into bag?
G2G tends to be faster from what I have read.
Or G2G from bag to bags.?
How mush spawn grain is better for G2G? For faster colonization.
Thanks for your time helping me.


--------------------
"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts



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