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OfflineVXSy
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Registered: 10/12/19
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Can you recommend where to transfer from?
    #27013454 - 10/31/20 08:46 AM (3 years, 3 months ago)

Hello all, just a quick ask if you can see anywhere I could take a transfer from?



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Offlineimperialshipworker
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Registered: 06/26/20
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Re: Can you recommend where to transfer from? [Re: VXSy]
    #27013526 - 10/31/20 09:57 AM (3 years, 3 months ago)

If this is the only plate you have to work with, I would divide that big tuft in the corner into multiple other plates, do a few more transfers, then select whichever one has the most rhizomorphic appearance... aka the most similar to the shape of trees or feathers.

What you SHOULD have done though, is only put one drop down from the syringe on to the middle of the plate, then roll it around to make a circle without touching the side of the plate though.  The water droplet will drag spores along with it.  You went overkill and it'll take extra work to separate and distinguish all the cultures you have bunched together in there.


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Offlineimperialshipworker
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Re: Can you recommend where to transfer from? [Re: imperialshipworker]
    #27013537 - 10/31/20 10:06 AM (3 years, 3 months ago)

Well.. better way to say it is "what works for many people and seems to be standard is the [yadayada] method" instead of "what you should have done is."


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Offlineverum subsequentis
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Re: Can you recommend where to transfer from? [Re: imperialshipworker]
    #27013686 - 10/31/20 11:53 AM (3 years, 3 months ago)

I prefer putting one drop on a flame sterilized loop and then streaking the plate. OP i like the growth That's just a little down and to the left from the center of the plate. I'd take two or three from there to new plates. Take nice small transfers and see how they grow out.


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OfflineVXSy
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Re: Can you recommend where to transfer from? [Re: verum subsequentis]
    #27013770 - 10/31/20 12:45 PM (3 years, 3 months ago)

I will try that clump! Many thanks


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Offlineimperialshipworker
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Registered: 06/26/20
Posts: 18
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Re: Can you recommend where to transfer from? [Re: VXSy]
    #27013809 - 10/31/20 01:05 PM (3 years, 3 months ago)

verum - yeah definitely using it to streak makes even more sense.  I've had good luck with both ways, I suppose I just like those maze games where you have to guide the ball out of the maze with gravity


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