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OfflineMycelionaut
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Registered: 06/20/20
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4th Transfer. Mycelium Still Dense, No Sectoring
    #27011307 - 10/29/20 11:55 PM (3 years, 3 months ago)

Good day everyone,

This is my first go at Print to Agar. This is now my 4th transfer and like the title says, the mycelium is still very dense and cottony, with no real sectoring...although I do see a few promising spots on one of the plates.

The mycelium is clean, so thats good! I'm just not seeing that nice, circular Tree of Life rhizo like I see from others here on the forum. Where do I go from here? I know rhizo isn't the ONLY goal, I'm just wondering at what point I simply let the mycelium take over 90% of the plate and take it to grain. Do I keep transferring until I get some promising strands? I'm afraid if I put this to grain now, even if I get good colonization and take it to a tub, I will end up with a thick, cottony cloud on top of the whole tub instead of what I have seen in the more successful grows.

I apologize for the lack of knowledge at this point, I dove into the deep end and am trying to learn as much as possible as I go. I got a little spoiled with my first Agar trial. I did an MSS to grain and as soon as I saw a few little spots of mycelium in the birdseed, I plucked one with an inoculation loop and it was like...beautiful the first "transfer". This Golden Teacher spore print is proving to need a little more TLC. Thanks in advance for any assistance!

Malt Extract Agar: 500ml water, 10g Agar, 10g MA
BOD's Comprehensive Agar Tek (if anyone may need that info)

I altered the pics to try and get the best representation of what's happening on the plates. They are perfectly white, on a light amber agar. These babies are hard to photograph, it turns out! Hahaha



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Offlinelookintolearn
Stranger becoming Strangest
I'm a teapot User Gallery


Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 2 months, 6 days
Re: 4th Transfer. Mycelium Still Dense, No Sectoring [Re: Mycelionaut]
    #27011340 - 10/30/20 12:31 AM (3 years, 3 months ago)

From what I can tell it's looking good to me. Just not rhizomorphic growth its more tomentose growth. That happens sometimes not all cultures get ropey with its mycelium. example:


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Offlineel gordo
not the youtube one
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Registered: 09/05/20
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Re: 4th Transfer. Mycelium Still Dense, No Sectoring [Re: lookintolearn]
    #27011390 - 10/30/20 02:21 AM (3 years, 3 months ago)

looks good, this kind of rhizo growth depends mostly on the strain and nutrients avaliability of the media mostly, less nutrients= more rhizo


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OnlineSingularFusion
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Registered: 10/31/18
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Re: 4th Transfer. Mycelium Still Dense, No Sectoring [Re: el gordo]
    #27011437 - 10/30/20 03:44 AM (3 years, 3 months ago)

yeah I use 2% malt also most of the time and rarely see the rhizo growth on my plates

however just because it looks fluffy there does not mean that is how it will necessarily act on grain, even the type of grain can lead to different morphology in the mycelia IME. Oats looking a bit wispy, that sort of thing

are you trying to isolate further or are you simply looking for clean organised growth to put to grain?

if it is the latter then I would say personally that you could use those plates, at least from what I can tell from here... but obviously you have to trust your own eye, those photos are not the highest detail and I am no expert of the craft

you can use a small piece of agar to inoculate grain jars, just takes a bit longer than a large piece or whole plate. So you could noc up some jars with smallish wedges and still leave enough good plate to make a few transfers to T5 and see if it is any different/better

or just take a little more time and a few more transfers if you are not in any sort of rush, maybe it gets better, maybe it doesn't. Possibly a change of media, less nutes etc may give you a better looking plate

but I dunno that it would actually make any difference to the end result in a tub is what I am saying, pretty plate or not

let us know how it goes eh


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OfflineBobbit
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Registered: 05/30/18
Posts: 1,250
Loc: The outsideround Flag
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Re: 4th Transfer. Mycelium Still Dense, No Sectoring [Re: Mycelionaut]
    #27011483 - 10/30/20 04:35 AM (3 years, 3 months ago)

Quote:

Mycelionaut said:
I apologize for the lack of knowledge at this point, I dove into the deep end and am trying to learn as much as possible as I go.




Keep diving. .  i.e. looks clean, take to grain. . . Learn. . . If yu get fruit, clone to agar, then back to grain. . . Rinse, repeat, learn. . .

Looks like you are well on your way :thumbsup:


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