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OfflineCaptainParanoid
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Registered: 08/06/20
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What to do with my agar?
    #27011018 - 10/29/20 07:15 PM (3 years, 2 months ago)

So I’ve got 6 agar plates. 4 of the plates are doing well, grew out pretty uniformly, no contamination on the plates. 2 of the plates have contamination but the mycelium growing on these plates look to be better. I might be wrong about this because I’m green so what do I know. Of the two contam plates one the mycelium is rhizo growth but very very tight. The other contam plate looks similar to the numbered plates but the rhizomorphic growth looks thicker in general (in person anyway, this might not be evident in the pics). I’ve also got two plates growing from a MSS.

So here’s my questions.

Looking at the 6 plates am I correct in thinking that the two plates with contamination look to be superior to the numbered plates? If so where should I be looking to transfer from. If I were winging it I’d just transfer from the edge as far from the contam because it’s all pretty even at this point, agree with that?
For the plates just starting out from a mss, any suggestions on what ro transfer beyond whatever appears to be growing the fastest?

Where would you more experienced people go from here? Expand a contam plate or both plates to more agar and put to grain? Or maybe ditch the contam plates and concentrate on the numbered plates?











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OfflineMyco Extremist
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Re: What to do with my agar? [Re: CaptainParanoid]
    #27012934 - 10/30/20 09:35 PM (3 years, 2 months ago)

If you have the agar to spare I would just take a transfer from each. If you want to go to grain you can just throw the 2 clean ones in jars after you transfer. As for the APE I am still trying to figure out where the hell to transfer it from myself, stuff grows so funny on agar. :grin:


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: What to do with my agar? [Re: Myco Extremist]
    #27013066 - 10/30/20 11:04 PM (3 years, 2 months ago)

the four plates in the first pic look great and ready for grain. It's hard to tell in pictures sometimes but i don't see any problems. The next pic looks good but i don't know what the fuck the arrow is pointing at. If it's a contam I'd take a transfer to a new plate and wait before putting it to grain.

I'm assuming the ape ms is from the same syringe from the same vendor. I'd probably take two transfers from the top one and nothing from the bottom. Not that there isn't potential. I just like a certain area on the top one. That area is about halfway from the center in the 2:30 (think hour hand on a clock) direction. Start at the center and proceed in that direction until you see a spot with no growth on the agar. The rhizo growth just before that is the best looking around (to me). I'd snatch two from there and put each in a new plate.

As always, some pics are better than others and it's kind of hard to diagnose myc through pics. But that's what i think from what i see here.

P.S. Good work friend. Looks like you're doing great.


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OfflineCaptainParanoid
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Re: What to do with my agar? [Re: verum subsequentis]
    #27013725 - 10/31/20 12:13 PM (3 years, 2 months ago)

The arrow was pointing to contam. I put the arrow there when it wasn’t so noticeable to keep an eye on it.  I transferred from the two contam plates of course.
For the two APE plates I made two transfers from two of the little colonies that looked like they were growing out the fastest. I’m not really worried about those plates. I plan on just playing around with them making transfers and maybe some experimenting while I wait for some of my other projects.


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InvisibleNecroMyce
Mostly Ghostly
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Registered: 05/12/13
Posts: 747
Re: What to do with my agar? [Re: CaptainParanoid]
    #27014096 - 10/31/20 03:29 PM (3 years, 2 months ago)

Looks good! I’d do what verum said


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