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InvisiblescienceMan
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A Beginner's Diary to Cloning Wild Cubensis
    #27009596 - 10/29/20 03:30 AM (3 years, 2 months ago)

Hey there it's my first post here.

I started a few months ago by isolating a wild strain of cubensis. I used a half-assed cloning technique by dropping pieces of mushroom into a sterilized grain jar, and it took me a while to clean it up by filling the jar with diluted h2o2 on repeated occasions, until all I saw was white mycelial fluff.

Well, it seems like it's been working and I'm finally trying to fruit these things (after lots of trial and error). It's a tropical rainforest here so... Contaminants fiesta!

(And I have to be a lot more paranoid than what seems to be normal for you all... H2o2 has become my best friend)

I decide to put a sample to agar a few days ago (from my grain jar), and what's happened on the agar is a little surprising...

The mycelium seems to have spread under the surface: I saw tiny individual hairs poking out of the agar, very sparse and spreading outwards and then very quickly it all grew out creating all this cottony fluff more or less simultaneously over the whole surface.





Here's one I put to agar two days ago... You can see the very sparse mycelium hair covering the whole plate.



That's nothing like I've seen anywhere.

Well, I haven't fruited anything yet so the judge is still out on whether I successfully isolated the cubensis I started with. But... It's all growing rather well and remarkably quickly.

So far I'm assuming I have a rather interesting strain of cubensis (or maybe it's not cubensis?)...

I wonder if anyone has any input on these pictures and on my experience so far?

Thanks a bunch... 🙏





PS: the black stuff in the jars is coffee and coir (I mixed wheat grass, coir, coffee, verm, and gypsum). There's no contamination anywhere (great success! 👍)...

.. and an interesting fact is that this mycelium doesn't seem to like coir and coffee very much. It's taking its sweet time to colonize the parts that have more of it over grains.


Edited by scienceMan (10/29/20 04:16 AM)


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OfflineSingularFusion
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009609 - 10/29/20 03:59 AM (3 years, 2 months ago)

I'm afraid the plates look like mold to me :shrug:

wait for a more experienced eye to take a look though

sorry dude


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: SingularFusion]
    #27009611 - 10/29/20 04:06 AM (3 years, 2 months ago)

I've been wondering about that, but that'd mean the stuff in the jars would have to be mold too... and it really doesn't look like mold would colonize a jar like that...🤔


Edited by scienceMan (10/29/20 04:14 AM)


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OfflineSold Out Online
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009619 - 10/29/20 04:16 AM (3 years, 2 months ago)

Fellow noob here, but that doesn’t look like any cubensis mycelium I’ve seen. You should have gone directly to agar plates and transferred away from the contamination. What you did was make h2o2 resistant something grow.


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: Sold Out Online]
    #27009621 - 10/29/20 04:18 AM (3 years, 2 months ago)

So my *guess* is that it's cubensis. I bought the shrooms from a local bar. I don't have pictures to show you unfortunately...

It's exciting, right? 😅


Edited by scienceMan (10/29/20 04:25 AM)


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InvisibleWall.E
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009627 - 10/29/20 04:28 AM (3 years, 2 months ago)

I can't advise you that is safe. Idk what you got going on but I'm super interested in hearing how filling up a jar of oats with hydrogen peroxide was supposed to get you healthy mycelium


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: Wall.E]
    #27009635 - 10/29/20 04:37 AM (3 years, 2 months ago)

Quote:

Myc_Hunt said:
I can't advise you that is safe. Idk what you got going on but I'm super interested in hearing how filling up a jar of oats with hydrogen peroxide was supposed to get you healthy mycelium




Peroxide kills bacteria and mold very quickly but leaves mushroom mycelium a little less dead...

Dose it right and you can clean a contaminated culture.


Edited by scienceMan (10/29/20 04:38 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009642 - 10/29/20 04:44 AM (3 years, 2 months ago)

Mycelium is a mold too. Peroxide doesn't go in there and pull a good guy killing only fungi we don't like...


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: bodhisatta]
    #27009645 - 10/29/20 04:49 AM (3 years, 2 months ago)

Well, it worked... 🙄

It's fairly common to see h2o2 being used to clean culture, in books as well as the internet...


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InvisibleWall.E
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009652 - 10/29/20 05:00 AM (3 years, 2 months ago)

Quote:

scienceMan said:
Well, it worked... 🙄

It's fairly common to see h2o2 being used to clean culture, in books as well as the internet...




Bod, I'm super new but that cake doesn't look healthy, right?


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InvisiblebodhisattaMDiscordReddit
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009653 - 10/29/20 05:01 AM (3 years, 2 months ago)

I don't see any mushrooms


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: bodhisatta]
    #27009665 - 10/29/20 05:20 AM (3 years, 2 months ago)

So... I'm just here to share the fun in sciencing this. Not really here to receive passive aggressive comments on my unorthodox methods.

The jar is healthy. The black stuff is uncolonized coffee and coir (I shook the jar a little three days ago cause the bottom wasn't colonizing fully). There's nothing but mushroomy smell coming from the jars with a hint of coffee. A couple jars have hints of yellowing due to metabolites.


Edited by scienceMan (10/29/20 05:22 AM)


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InvisibleWall.E
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009667 - 10/29/20 05:25 AM (3 years, 2 months ago)

Alright then keep us updated


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: Wall.E]
    #27009695 - 10/29/20 06:12 AM (3 years, 2 months ago)

Thanks bro.

I should add that whatever is growing here came directly out of the pieces of mushroom I put in there originally (many months ago).

The h2o2 killed everything in the bottle but the mycelium that still lived inside the mushroom pieces, as best I can tell...

So for now that kind of makes me hopeful something will grow out of this ☺️


Edited by scienceMan (10/29/20 06:13 AM)


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InvisibleRoger Clemency
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009724 - 10/29/20 06:53 AM (3 years, 2 months ago)

Almost every time I’ve seen a new grower use the words “it’s not contaminated” or anything with that sentiment it’s a subconscious hopeful type statement that is totally unfounded. Not just trying to rag on you but I’ve seen it like 18 times with situations very similar to this.

If you’re having fun with what you’re doing and just wanna see what happens then that’s cool and ignore the rest but if you want to grow mushrooms you should start another grow following more standard procedure.

I’m very into the idea of growing out wild cubes (though I don’t think a bar counts lol, it’s probably a stabilized variety that some guy grew nearby) and looking for clones and stuff but why wouldn’t you just go straight to agar with the original mushroom? And if it was a dry mushroom from a baggie that you dropped into a jar of grains...:eek:

Get some spores, swipe to agar, eat mushrooms.


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InvisiblebodhisattaMDiscordReddit
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Re: Fluffy Mycelium Mystery [Re: Roger Clemency]
    #27009733 - 10/29/20 07:04 AM (3 years, 2 months ago)

I would be very surprised if those cakes fruit and if they do it won't be more than a couple grams


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OfflineA.k.aM
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Re: Fluffy Mycelium Mystery [Re: bodhisatta]
    #27009755 - 10/29/20 07:37 AM (3 years, 2 months ago)

I’ve got jars of mold that look just like that right now. It’s really hard to tell sometimes when you get white mold.

Based on the plates though I wouldn’t expect much, anything that spreads in two days isn’t good.


Hopefully it works out though I like seeing wild grows they always seem to be way different looking than the usual stuff.


I also think you’d be better off printing a wild cap or just taking a clean tissue sample to agar in a SAB would be quickest.


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InvisiblescienceMan
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Re: Fluffy Mycelium Mystery [Re: A.k.a]
    #27009932 - 10/29/20 09:17 AM (3 years, 2 months ago)

I dug around a little and now I think I might have cloned from pan cyan (which I wasn't familiar with until I just saw some pictures of it). They kinda look like thin cubes, don't they?

And my plates look a lot like this...

Oh and I couldn't get a print, the shrooms I get are always refrigerated and not in a print-frienslt state... I'm doing the best I can with what I got... ☺️

https://www.shroomery.org/forums/showflat.php/Number/4095334

Anyone here grown pan cyan before?


Edited by scienceMan (10/29/20 09:19 AM)


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InvisibleWall.E
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Re: Fluffy Mycelium Mystery [Re: scienceMan]
    #27009970 - 10/29/20 09:40 AM (3 years, 2 months ago)



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