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Offlineevicsur
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First time grower - PF TEK contaminated?
    #27007911 - 10/28/20 09:03 AM (3 years, 2 months ago)




Inoculated on 10/22, noticed these dark spots in some of my mycelium growth on 10/25 when I was moving jars to a warmer spot.

Over the last three days the dark bits aren't spreading/growing as far as I can tell but it seems like it's occuring in tiny spots in a few other places, always within the mycelium (though that may just be the substrate touching the glass). It also only appears to be where mycelium is directly touching the glass.

I used MSS from (vendor removed per forum rules), pc'd my jars and substrate for 90 mins (PC only went to 12/13, couldn't do 15), worked in a SAB and torch sterilized the needle before starting then every 2 jars. Really hoping I didn't STILL manage to get them contaminated. The jars I have noticed it in are my 2nd, 3rd and 9th inoculated.

Should I let them be for now and see how the jars fill in or are they dead in the water? The mycelium appears to still be growing great in the jars that show this.


Edited by evicsur (10/28/20 07:24 PM)


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InvisibleDarkshadow
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Registered: 06/11/20
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Re: First time grower - PF TEK contaminated? [Re: evicsur]
    #27008128 - 10/28/20 11:10 AM (3 years, 2 months ago)

I would leave those be for now, but keep an eye on them.


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InvisibleRhizomorph
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Registered: 04/24/20
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Re: First time grower - PF TEK contaminated? [Re: evicsur]
    #27008233 - 10/28/20 12:02 PM (3 years, 2 months ago)

https://www.shroomery.org/forums/showflat.php/Number/26679862#26679862
Here's a thread with what looks like the same thing in your jars.

I think it is some type of mold but it doesn't seem very aggressive and you may still be able to get some decent fruits.

Give it time and if it spreads toss them.


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Offlineevicsur
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Re: First time grower - PF TEK contaminated? [Re: Rhizomorph]
    #27008422 - 10/28/20 01:32 PM (3 years, 2 months ago)

Quote:

Here's a thread with what looks like the same thing in your jars




Wow, yeah that looks EXACTLY like what I have and it's like the same circumstances. The jars that have it are showing some truly gorgeous mycelium growth while the spots stay the same.

If this continues I'll open the spotted ones in another room and try to wipe the spots off before I shoebox them together - the rest will go in a SGFC. I'm also gonna start a couple Uncle Bens bags too as an extra assurance of SOMETHING being successful. Def gonna look into going LC or agar with any successes.


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InvisiblefahtsterM
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Re: First time grower - PF TEK contaminated? [Re: evicsur]
    #27008598 - 10/28/20 02:49 PM (3 years, 2 months ago)

Was your spore syringe really dark with spore clumps?  Good chance that it’s just a clump of spores... I had that recently with a PE syringe

Faht


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Offlineevicsur
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Re: First time grower - PF TEK contaminated? [Re: fahtster]
    #27008752 - 10/28/20 04:21 PM (3 years, 2 months ago)

Quote:

Was your spore syringe really dark with spore clumps?  Good chance that it’s just a clump of spores





Yeah it had some pretty chunky bits I couldn't break up. Would they turn black from oxidation?

I was super careful to work as sterile as possible and I've heard way too many people rave about the vendor to think I got something crazy contaminated from them. Time will tell I guess, but for now they seem happy enough. I'll see how they look in like a week.


Edited by evicsur (10/28/20 07:23 PM)


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InvisiblefahtsterM
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Re: First time grower - PF TEK contaminated? [Re: evicsur]
    #27009001 - 10/28/20 06:42 PM (3 years, 2 months ago)

Please don’t use vendor names in posts associated with growing.. site rules.  Thnx

I’d bet the rent they’ve been there since you inoculated the jars and just didn’t realize till spores started growing from it.  Here’s a PE cake that had a crazy amount of spores and clumps in it.. I watched the clumps go into the cake..



Here’s the syringes.  Definitely has a black feel to it



Just don’t toss the jar lol

Faht


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Offlineevicsur
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Re: First time grower - PF TEK contaminated? [Re: fahtster]
    #27009091 - 10/28/20 07:23 PM (3 years, 2 months ago)

My bad on the name, I didn't realize!

Thanks for the insight, definitely gonna keep them growing and monitor. The pics on my post are from a couple days ago and the myc in those jars has easily doubled or more already!


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