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mushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: GrinchGrower]
#27092938 - 12/16/20 08:01 PM (3 years, 1 month ago) |
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I believe that with the josex poke, you should give it ample time to recover before spinning. You see the sample is so microscopic that spinning it too early could possibly kill the culture.
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Josex
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
#27092952 - 12/16/20 08:07 PM (3 years, 1 month ago) |
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spinning early makes no sense, it's counterproductive IMO. It's as if you shook a jar when the wedge has barely got time to leap off.
Good all swirling works pretty good tho, can't see no good reason to dick around with stirrers even tho I dick around with stirrers.
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mushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
#27092973 - 12/16/20 08:15 PM (3 years, 1 month ago) |
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Stirred a culture two days after using your method and killed it. Good news is I got to reuse the LC LOL
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GrinchGrower
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
#27093058 - 12/16/20 08:56 PM (3 years, 1 month ago) |
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I'll let them sit longer next time.
Blue Helix mentioned that spinning early might cause issues, but recommended just letting it sit a day or two.
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Lemgrub



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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: GrinchGrower]
#27093097 - 12/16/20 09:15 PM (3 years, 1 month ago) |
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Lookin good. 5 day difference. 1ml LC to ~400ml broth 11/9

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mushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
#27099185 - 12/20/20 11:26 AM (3 years, 1 month ago) |
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   Just wanted to share progress on this lc. I stole pasty's idea and made up a no-pour agar vessel, colonized it with my PESA culture, and added some sterile low nute LME LC.
Edited by mushhead (12/20/20 04:00 PM)
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D3_Myc
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead] 1
#27099201 - 12/20/20 11:38 AM (3 years, 1 month ago) |
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I took a transfer from that liquid culture streak test I posted above... doesn’t get much sexier than this

LC back to agar isnt so bad thats for sure
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mushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: D3_Myc]
#27099263 - 12/20/20 12:27 PM (3 years, 1 month ago) |
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  Gorgeous!
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Lenz
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
#27101307 - 12/21/20 05:25 PM (3 years, 1 month ago) |
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 '
Tex Orange Cap LCs inoculated with Josex poke looking decent. The second one is a bit more difficult to break up just by hand swirling, don't know what I'm gonna do when it's actually time to pour these but oh well lol. Need to test them first anyways. Really enjoying this first try at LC though.
Edited by Lenz (12/21/20 05:25 PM)
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MH5109
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lenz]
#27101349 - 12/21/20 06:06 PM (3 years, 1 month ago) |
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I left my peu lc on the stir plate for what was supposed to be 10min and I fell asleep and then went to work, which turned into almost 20hrs. She was pretty minced up definitely mixed.
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Luminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: MH5109]
#27129402 - 01/05/21 10:21 PM (3 years, 22 days ago) |
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How many quart jars of grain should 500ml of thick LC colonise?
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Josex
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
#27129410 - 01/05/21 10:29 PM (3 years, 22 days ago) |
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Maybe you would not want to prep enough grain for that much LC. 5ml per quart jar is already a generous amount of LC, so you could knock up 100 jars with a 500ml LC. Want to stretch it out a bit? Do 1ml per jar and inoculate the fucking world.
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Luminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
#27129425 - 01/05/21 10:38 PM (3 years, 22 days ago) |
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Quote:
Josex said: Maybe you would not want to prep enough grain for that much LC. 5ml per quart jar is already a generous amount of LC, so you could knock up 100 jars with a 500ml LC. Want to stretch it out a bit? Do 1ml per jar and inoculate the fucking world.
At 5ml a jar will it colonise in 10-13 or so days though?
I have been using about 20-25ml per jar, its a bit wet but colonises in 10 days give or take.
Im starting to think this is wasteful. lol
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Josex
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
#27129436 - 01/05/21 10:46 PM (3 years, 22 days ago) |
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It behoves you wait a bit more for full colonization and not throw off the moisture content of your jars imo. 20-25 is definitely a lot for jars, some people do less than that for bags.
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Luminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
#27129440 - 01/05/21 10:48 PM (3 years, 22 days ago) |
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Quote:
Josex said: It behoves you wait a bit more for full colonization and not throw off the moisture content of your jars imo. 20-25 is definitely a lot for jars, some people do less than that for bags.
i see. Safer on the dryer side eh?
WIll it still colonise in a half decent time that low?
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Josex
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
#27129456 - 01/05/21 10:55 PM (3 years, 22 days ago) |
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I'd say 10-13 days is pretty normal from 5ml. In summer I've seen jars done in as little as 8 days from 5ml and it's not rare to see jars done in 6 days . As soon as temps go down colonization can slow down a lot too. Make sure that your grains are correctly hydrated and it'll all colonize faster. I've seen people recommending prepping grains on the dry side just so they could use more LC, this is a terrible thing to do.
Edited by Josex (01/05/21 11:04 PM)
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Luminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
#27129507 - 01/05/21 11:37 PM (3 years, 22 days ago) |
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Quote:
Josex said: I'd say 10-13 days is pretty normal from 5ml. In summer I've seen jars done in as little as 8 days from 5ml and it's not rare to see jars done in 6 days . As soon as temps go down colonization can slow down a lot too. Make sure that your grains are correctly hydrated and it'll all colonize faster. I've seen people recommending prepping grains on the dry side just so they could use more LC, this is a terrible thing to do.
Word, I think im starting to see where Iv been a bit off myself.
THanks for the insight dude .
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The Dalcassian
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
#27151429 - 01/16/21 12:40 PM (3 years, 11 days ago) |
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Probably a dumb question but does an LC need GE?
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GrinchGrower
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: The Dalcassian]
#27151478 - 01/16/21 01:03 PM (3 years, 11 days ago) |
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None of my bottles have any GE and seemed to colonize okay. Just poured some on grain and streaked to agar yesterday, so I guess we will see...
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The Dalcassian
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: GrinchGrower]
#27151503 - 01/16/21 01:17 PM (3 years, 11 days ago) |
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Only curious as I've had a pan jar nocc'd with LC doing nothing for nearly two weeks then I shook it. Found there was a tiny bit of colonisation in the center which has since exploded with growth.
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