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InvisiblePastywhyteMFacebookDiscord
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27071691 - 12/04/20 01:52 PM (1 year, 1 month ago)

I sometimes let them get super thick and just pour globs of mycelium right into the bag. It often recovers in less than a day in those instances.


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Invisiblecoversall
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27071802 - 12/04/20 02:45 PM (1 year, 1 month ago)

Ace!


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OfflineCamera93
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27071813 - 12/04/20 02:52 PM (1 year, 1 month ago)

I just used a month+ old LC that sat at room temp
300 or so ml in a media bottle, poured to 32 quarts around midnight on the 3rd
I hope to see recovery today when I get home, around 5pm will be 41 hours since inoculation


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Invisiblecoversall
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27071828 - 12/04/20 02:57 PM (1 year, 1 month ago)

Quote:

Pastywhyte said:
I sometimes let them get super thick and just pour globs of mycelium right into the bag. It often recovers in less than a day in those instances.



:wellhello:


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Offlinekanemush
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27075372 - 12/06/20 04:08 PM (1 year, 1 month ago)

it has been almost 3 days since a noced 1g lme 250ml of water. this is my second try at a broth. I just started stirring these 2 days ago.



Edited by kanemush (12/06/20 04:09 PM)


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InvisibleD3_Myc
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Sold Out Online]
    #27075456 - 12/06/20 04:56 PM (1 year, 1 month ago)

Quote:

cdnpsychonaught said:
Quote:

D3monic said:


What method did you use to get it to agar to test?





I just poured a little onto a petri lid and then hit it with the loop

Here’s my expanded cultures, going to send some more to grain and expand again in a day or two.



Looks like lme has been a clear winner in all my tests though I do like the grain water as well.



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OfflineLemgrub
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: D3_Myc]
    #27084443 - 12/11/20 07:54 PM (1 year, 1 month ago)

I decided to add a tiny bit of agar to a broth I used for LC to see how it would turn out.

I inoculated this on 12/8 from a tissue biopsy LC that showed to be clean after a agar test a while ago.

The agar is so loose in this LC it looks close to bacterial, but keeps the mycelia suspended for a while after a few swishes which may help it grow:ohwell:

I used about 1ml of the other LC and could barely tell there was any myc in there at first, seems to be growing pretty fast.



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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27084928 - 12/12/20 03:25 AM (1 year, 1 month ago)

So I decided to give LC a go and am pretty pleased with this so far;



It's growing in a uniform ball shape, do I need to try and break this up with a swirl or just let it do it's thing?

You can't see it from this pic but the myc is growing of a dog hair that accidentally got PC'd in the bottle lol.

I know distilled water would give me a clearer medium but I said I'd just give it a go with what's on hand first to see if I like working with it.


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: The Dalcassian]
    #27084931 - 12/12/20 03:33 AM (1 year, 1 month ago)

That Jellyfish needs shook! Needed it days ago. I have a hard time watching that thing, my ocd kicks in lol it's so big I feel like reaching through screen and shaking the shit out of it.


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OfflineThe Dalcassian
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27084945 - 12/12/20 03:49 AM (1 year, 1 month ago)

Oh crap lol, ok I'm on it.

You have made me chuckle tho, thanks:laugh2:

Edit; Sorted, although now it floats



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Edited by The Dalcassian (12/12/20 04:00 AM)


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: The Dalcassian]
    #27084950 - 12/12/20 03:58 AM (1 year, 1 month ago)

It's looks healthy man, that's the important thing. :cheers:


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27084955 - 12/12/20 04:06 AM (1 year, 1 month ago)

Thanks dude I look forward to getting to use it.


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex] * 1
    #27084959 - 12/12/20 04:12 AM (1 year, 1 month ago)

I also made a APE LC from a agar chunk on a MS plate 12/2. The main chunk stuck to the side of the wall of the cup but it's been seeding off new colonies.



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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27089314 - 12/14/20 08:18 PM (1 year, 1 month ago)

Thoughts on an LC that grows very slowly? I recently discarded an LC that grew but was just very slow. Could this be a sign of contamination or just genetics?


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: bongoman]
    #27089384 - 12/14/20 09:05 PM (1 year, 1 month ago)

slow/poor recovery is a bad sign for anything no matter the media.


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushboy]
    #27092144 - 12/16/20 03:04 PM (1 year, 1 month ago)

Commenting mainly to follow up later...

  • I made two .4% LME (1g to 250ml deionized water) and PC'd for 17 minutes @ 16-17 PSI on 12/13.
  • Inoculated both with Josex poke method on 12/14 using my best (AC) plate.
  • I let them sit for a day before spinning a couple hours each on low yesterday and today.
  • Just seeing cloudy sediment so far and some random detritus that was probably in the medium during PC (hopefully).






Still not sure how to get good pictures of these... maybe bottom lighting instead?


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27092160 - 12/16/20 03:09 PM (1 year, 1 month ago)

Running low on plates so I just used some peu lc without testing first. :rockon:


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: p9hu7]
    #27092667 - 12/16/20 07:50 PM (1 year, 1 month ago)

Ziploc sandwich bag agar. I had a little bit of agar left after pouring 40 plates. I didn't want to waste the agar so I thought about what I wanted to do with the rest of it. I remembered a friend of mine telling me a few years ago about ziploc bags being packaged sterile and that one could use them for pouring agar. It actually works really well.


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27092911 - 12/16/20 09:53 PM (1 year, 1 month ago)

I use ziploc sandwich bags in lieu of wrapping poured plates.


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27092932 - 12/16/20 09:59 PM (1 year, 1 month ago)

That sounds about ez, would hate to wrap plates myself, main reason I never got started with petris.


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