Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Next >  [ show all ]
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear] * 2
    #27053746 - 11/23/20 10:52 AM (3 years, 2 months ago)



60ml syringes wrapped in foil and a pp5 test tube full of 14G needles wrapped in foil, all sterilized inside a gallon jar.


Extras: Filter Print Post Top
InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27053765 - 11/23/20 11:04 AM (3 years, 2 months ago)

This is all good to know, currently I’m working with just pouring in front of the flowhood but planned on making a few lids with injection ports and getting some syringes. Is there a favorite brand / size of syringe? (I’ll utilize the search button as well)


Extras: Filter Print Post Top
Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27054499 - 11/23/20 07:23 PM (3 years, 2 months ago)

Quote:

mushhead said:

Left is B+ Right is APE.
LME heat dissolved into distilled water with a 45min PC cycle.
Some sediment but no other microbes save mycelia in here.




B+ (Left) APE (Right)

A couple other LC testing nute levels.


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27054525 - 11/23/20 07:41 PM (3 years, 2 months ago)

From my limited knowledge about those broths (only knowing these pics and what you've described so far) I'd say B+ looks turbid APE looks clear. But it's worth remembering that discernment comes from having the collective data on everything from time of inoculation, temp, changes in appearance no matter how slight, timing of the changes, many many small factors that I myself know very little about I'm totally a baby just discovering as I go.


I'm just pointing out that the one factor we have that appears to be a very rigid measuring device (the turbidity phenomena itself) nevertheless eyes can also be tricked, I've already been punked by some broths a few times, so observing them regularly in the hours/days after inoculation tends to provide the most valuable data ime.


--------------------
Cakes inside Water Tub


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,808
Loc: Canada Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27054535 - 11/23/20 07:47 PM (3 years, 2 months ago)

I like these, they look ready to rip into some grain.





Extras: Filter Print Post Top
Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27054542 - 11/23/20 07:52 PM (3 years, 2 months ago)

Quote:

mushhead said:
I've been working on my LC nute levels. Trying to get them as clear as everyone's here.
My LCs are clean, but have sediment because I've been experimenting with nute levels.
My first batch is 5g LME to 500ml distilled water. LOTS of sediment. 
Second is 2.5g LME to 500ml distilled water. Medium-minor sediment.
Third is 1g LME to 500ml distilled water. Mostly none. Small amount visible on bottom of jar.
Fourth is .5g LME to 500ml distilled water. Pretty clear.
Pics after I finish a bunch of errands today.




I PC for 45min each batch of two jars I did. The fourth batch is just the one jar.
They're in succession from first batch to fourth which I'm sure you can tell which has the highest nutes.
You see I believe that the B+ jar isn't contaminated at all and the turbidity comes from the amount of nutes used which is like 5~6g because it was my first one after trying with Gulf coast which worked amazingly well for me.
EDIT: These jars were kept at 22c consistently without deviation.


Edited by mushhead (11/23/20 08:15 PM)


Extras: Filter Print Post Top
Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27054558 - 11/23/20 08:02 PM (3 years, 2 months ago)

Quote:

Pastywhyte said:
I like these, they look ready to rip into some grain.







Pasty I aspire to be upon your level immensely.


Extras: Filter Print Post Top
InvisibleLemgrub
 User Gallery

Registered: 04/02/18
Posts: 589
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27054565 - 11/23/20 08:06 PM (3 years, 2 months ago)

Got a APE syringe in today, first time working with it. But it looks like they germinated...



--------------------
We getting that pirate bay, alien shishkebab cordycep money


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27054581 - 11/23/20 08:16 PM (3 years, 2 months ago)

The pic isn't good but Ape spores kinda look like that. If they take time to show growth on agar (and they usually will) you can be sure it's spores.


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27054582 - 11/23/20 08:17 PM (3 years, 2 months ago)

Quote:

Lemgrub said:
it looks like they germinated...





I'm afraid "they" could mean 'several types of organisms' germinating. Both fungus myc and bacteria is what the pic says to me. I wonder if it'd be possible to salvage a part of the myc in there tho, maybe to dilute a small part heavily in sterile water and then to extract a samle to put to agar? What do y'all think about this guys? I'm sure someone has tried something similar before, to cleaning out myc from a bacterial broth lol


--------------------
Cakes inside Water Tub


Extras: Filter Print Post Top
InvisibleLemgrub
 User Gallery

Registered: 04/02/18
Posts: 589
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27054625 - 11/23/20 08:44 PM (3 years, 2 months ago)

About as good a pic as I can get. looking closer I can tell there are spores, but it looks a bit more viscous than usual. I nocced some agar and brf cakes and a glob came out in one of the cakes. I've looked at plenty of spores under the scope and I've seen a large mass of spores sort of suspended in a matrix of hyphal cells, I don't know if they're just dead in some way or if they were sterile monokaryotic mutant lineages or something like that.




--------------------
We getting that pirate bay, alien shishkebab cordycep money


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,808
Loc: Canada Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27054658 - 11/23/20 09:28 PM (3 years, 2 months ago)

Looks like APE spores to me.


Extras: Filter Print Post Top
InvisibleLemgrub
 User Gallery


Registered: 04/02/18
Posts: 589
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27054730 - 11/23/20 10:49 PM (3 years, 2 months ago)

Tight

Here's a tissue needle biopsy LC I started 11/13 from Colombian Rust. The liquid has retained the same clarity throughout. Believe it's growing somewhat slowly due to higher sugar content, 4%



--------------------
We getting that pirate bay, alien shishkebab cordycep money


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27054744 - 11/23/20 10:58 PM (3 years, 2 months ago)

Are you swirling it? That will break up that glob and speed up colonization. It's being terribly slow regardless, I would not trust it but you can always test it.


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27054790 - 11/23/20 11:49 PM (3 years, 2 months ago)

Quote:

Lemgrub said:
Tight

Here's a tissue needle biopsy LC I started 11/13 from Colombian Rust. The liquid has retained the same clarity throughout. Believe it's growing somewhat slowly due to higher sugar content, 4%





I've done side by sides with clones before and a varience in nutrient density didn't seem to affect growtht speed much if at all. At least not with the cultures I tested. The myc colony in your LC is far to small for a 10day old LC, it should really be close to being fully colonized at this point. This raises some concerns. Also (and I could be wrong) but from the pic it doesn't look like the light source is very close to the jar so it's hard to determine how clear/turbid that broth is. The brighter the light is shining on a turbid broth the more turbid it will appear and the less light that is being diffused inside the broth the less turbid it will appear so the amount of light shining through will hugely affect how the broth is perceived ime. And lastly I have to say that personally I can not come up with a reason to use stronger broths than 0.1%.

Adding a massive amount of particles to a LC like 2-4% malt will make it that much harder to inspect since you don't have a 'default' like a crystal clear broth for example in which case you're able to notice the slightest increase in particles (since the broth goes from 'clear' to 'not clear') but with a broth with already tons of particles in it then it's simply not reasonable to assume you'd even be able to notice a change in amount of particles (relying on noticing the change from 'turbidity' to 'more turbidity'. Hope that helps. Gl


--------------------
Cakes inside Water Tub


Edited by Mateja (11/23/20 11:53 PM)


Extras: Filter Print Post Top
Offlinesonoramo
Contaminant
Male User Gallery


Registered: 02/27/19
Posts: 851
Loc: California, baby! Flag
Last seen: 2 days, 4 hours
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27055222 - 11/24/20 09:53 AM (3 years, 2 months ago)

Quote:

Pastywhyte said:
I like these, they look ready to rip into some grain.








Is the brownish stuff on the bottom sediment? If it is, it seems like a lot of sediment. Like, if you swirled the jar it would make everything look cloudy. Was it there from when you prepared it, or did it precipitate out while the culture grew?

Finally, what recipe did you use for the broth?


Extras: Filter Print Post Top
InvisibleLemgrub
 User Gallery


Registered: 04/02/18
Posts: 589
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27055238 - 11/24/20 10:03 AM (3 years, 2 months ago)

I gotcha. The (very bright)lamp was probably an inch from the cup. I've had a few LCs contaminate since making this and the difference is stark. I don't believe this one is contaminated to this point.

And as far as the solution I used, I quickly looked at a tek that recommended 4%, so I went ahead and did it since the PC was almost ready to go. Seemed high and further reading supports that. And honey isn't too bad particle wise.

Josex, yes I've been swirling a couple times a day. Finally a few days ago some myc broke off from the biopsy and there's separate colonies now. I think the colonization will speed up now.


--------------------
We getting that pirate bay, alien shishkebab cordycep money


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,808
Loc: Canada Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: sonoramo]
    #27055256 - 11/24/20 10:08 AM (3 years, 2 months ago)

Quote:

sonoramo said:
Quote:

Pastywhyte said:
I like these, they look ready to rip into some grain.








Is the brownish stuff on the bottom sediment? If it is, it seems like a lot of sediment. Like, if you swirled the jar it would make everything look cloudy. Was it there from when you prepared it, or did it precipitate out while the culture grew?

Finally, what recipe did you use for the broth?




The brownish stuff on the the bottom is a giant puck of solid agar. The “broth” is simply sterile water added to the vessel once the colony on the agar has grown out and looks healthy. Use whatever recipe you like, this was just undiluted grain soak water and 2% agar for the puck.


Extras: Filter Print Post Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
 Unread Journal


Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Pastywhyte]
    #27055286 - 11/24/20 10:23 AM (3 years, 2 months ago)

Chucked an agar wedgelet into some LME broth today... It floated... I-is that ok?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


Extras: Filter Print Post Top
InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27055318 - 11/24/20 10:47 AM (3 years, 2 months ago)

Started some enigma on LC last night. T1 from a hot pour (it wasn’t needed just wanted to try it) This is one in a series of side by sides I’m going to try with grainwater and lme. My hypothesis is that the enigma will be more aggressive in grainwater based on its growth patterns on agar vs when it goes to grain. I’m looking forward to seeing the results



Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Visual diff between contam and Myc. for newbie? Pachanguero 2,145 4 06/18/05 06:34 AM
by drugsaregood
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 9,458 44 05/26/05 01:36 PM
by Aeolus1369
* Whats are some of more visual cube strains?
( 1 2 3 all )
Gr0wer 7,947 57 07/15/04 12:55 PM
by Ice House Shaman
* temperature drops as a cue for pinning TheChiaPetFarmer 3,246 13 08/19/04 09:02 AM
by deafpanda
* nice visuals? Gunnthier 1,797 16 02/10/05 10:37 PM
by casper7512
* visuals and potency bob_smith93456 1,113 13 09/06/05 06:28 PM
by bob_smith93456
* Cakes and LC's. Couple Questions. HippieChick 828 5 06/23/05 10:03 PM
by CLUTCH
* LC's for those stingy with their syringes. Or cloning straight to a LC blackout 1,039 1 07/21/05 01:01 PM
by scatmanrav

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
7,245 topic views. 18 members, 126 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.006 seconds on 13 queries.